Spirochaeta thermophila

Spirochaeta thermophila is a fairly recently discovered free-living, anaerobic, spirochaete that seems to be the most thermophilic of the Spirochaetales order.[1][2] The type species was discovered in 1992 in Kuril islands, Russia and described in Aksenova, et al.[3][4] It has been isolated in the sediments and water columns of brackish aquatic habitats of various ponds, lakes, rivers, and oceans.[1] This organism is identified as a new species based on its unique ability to degrade cellulose, xylan, and other α- and β-linked sugars and use them as the sole carbon source by encoding many glycoside hydrolases.[1][2][5] It is presumed to secrete cellulases to break down plant-matter around it but there has been little work on the characterization of the enzymes responsible for this.[2]

Spirochaeta thermophila
Scientific classification
Domain: Bacteria
Phylum: Spirochaetota
Class: Spirochaetia
Order: Spirochaetales
Family: Spirochaetaceae
Genus: Spirochaeta
Species:
S. thermophila
Binomial name
Spirochaeta thermophila
Aksenova 1992

Original description

The original description depicts single, helical, .2-.25 μm by 16-50 μm Gram-negative cells.[3] The temperature range for survival of Spirochaeta thermophila is between 40° and 73° C with an optimum range between 66° and 68 °C.[2][3] The pH range for survival was measured from 5.9 to 7.7 with an optimum of 7.5.[3] The G + C content measured was approximately 52% in the 1992 description but has been measured around 70% since that time.[1][3] The original description also noted that organisms of the same species isolated from different environments could have different optimum temperatures, optimum pH for growth, and optimum saline concentrations; these would change based on the environment in which the organism is living.[3]

Glucose fermentation pathway

The fermentation process is the same Embden-Meyerhof-Parnas pathway of glycolysis with the exception of one step.[4] The phosphorylation of fructose-6-phosphate mediates the production of pyrophosphate-dependent (PPi-dependent) phosphofrucktokinase instead of the usual ATP-dependent phosphofrucktokinase.[4] This appears to be a characteristic of Spirochaeta thermophila not found in other Spirochaeta species.[4] It is suggested that this different product could be a regulatory mechanism for catabolic processes; with low levels of the ATP-dependent molecule, AMP is produced.[4] AMP drives the production of PPi production for the step that is changed in the glycolysis pathway.[4]

The PPi-dependent phosphofrucktokinase sequences are only available from three organisms in the Spirochaetales order: Spirochaeta thermophila, Borrelia burgdorferi, and Treponema pallidum.[6] Comparing the sequences, in a 2001 study by Rominus et al., it was determined that S. thermophila was most closely related to T. pallidum for this sequence and the sister to those groups was B. burgedorferi.[6] This analysis showed the thermophily of S. thermophila and T. pallidum arose from a common ancestor between them and B. burgdorferi that was a mesophile.[6] This was an interesting revelation because it was previously assumed that the thermophilic, free-living spirochaetes gave rise to all extant spirochaetes.[6]

References
  1. Angelov, Angel; et al. (December 2010). "Genome Sequence of the Polysaccharide-Degrading, Thermophilic Anaerobe Spirochaeta thermophila DSM 6192". Journal of Bacteriology. 192 (24): 6492–6493. doi:10.1128/JB.01023-10. PMC 3008529. PMID 20935097.
  2. Bergquist, Peter L.; et al. (July 1998). "Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria". Federation of European Microbiological Societies: Microbial Ecology. 28 (2): 99–110. doi:10.1111/j.1574-6941.1999.tb00565.x.
  3. Aksenova, Helena Yu; et al. (January 1992). "Spirochaeta thermophila sp. nov., an Obligately Anaerobic, Polysaccharolytic, Extremely Thermophilic Bacterium". International Journal of Systematic Bacteriology. 42: 175–177. doi:10.1099/00207713-42-1-175.
  4. Janssen, Peter H.; Morgan, Hugh W. (April 1992). "Glucose Catabolism by Spirochaeta thermophila RI 19.B1". Journal of Bacteriology. 174 (8): 2449–2453. doi:10.1128/jb.174.8.2449-2453.1992. PMC 205880. PMID 1556064.
  5. Hespell, Robert B. (1994). "Xylanolytic Activities of Spirochaeta thermophila". Current Microbiology. 29 (6): 343–347. doi:10.1007/BF01570227. S2CID 24239152.
  6. Rominus, Ron (March 2001). "Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila". Archives of Microbiology. 175 (4): 308–312. doi:10.1007/s002030100265. PMID 11382227. S2CID 13550205.
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