Aspergillus niger

Aspergillus niger is a mold classified within the Nigri section of the Aspergillus genus.[1] The genus Aspergillus consists of common molds found omnipresent in the environment like on vegetation, within soil and water, in fecal matter, on decomposing matter, and suspended in the air.[2] Species within the Aspergillus genus are often fast growing and can sporulate within a few days after germination.[2] A. niger is a filamentous fungus capable of growing in a variety of environments due to its versatile metabolism and robustness to extreme acid environments, which is one reason why the specific mold is of great economic importance due to its industrial use in citric acid production.[1][3]

Aspergillus niger
Micrograph of A. niger grown on Sabouraud agar
Details of the head
Scientific classification
Kingdom: Fungi
Division: Ascomycota
Class: Eurotiomycetes
Order: Eurotiales
Family: Trichocomaceae
Genus: Aspergillus
Species:
A. niger
Binomial name
Aspergillus niger
van Tieghem 1867
Synonyms

Aspergillus niger var. niger
Aspergillopsis nigra (Tiegh.) Speg.
Rhopalocystis nigra (Tiegh.) Grove
Sterigmatocystis nigra (Tiegh.) Sacc. (1877)

It causes a disease called "black mold" on certain fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. It is ubiquitous in soil and is commonly reported from indoor environments, where its black colonies can be confused with those of Stachybotrys (species of which have also been called "black mold").[4]

Some strains of A. niger have been reported to produce potent mycotoxins called ochratoxins;[5] other sources disagree, claiming this report is based upon misidentification of the fungal species. Recent evidence suggests some true A. niger strains do produce ochratoxin A.[4][6] It also produces the isoflavone orobol.

Taxonomy

Aspergillus niger is included in Aspergillus subgenus Circumdati, section Nigri. The section Nigri includes 15 related black-spored species that may be confused with A. niger, including A. tubingensis, A. foetidus, A. carbonarius, and A. awamori.[7][8] In 2004 a number of morphologically similar species were described by Samson et al.[8]

In 2007 the strain of ATCC 16404 Aspergillus niger was reclassified as Aspergillus brasiliensis (refer to publication by Varga et al.[9]). This has required an update to the U.S. Pharmacopoeia and the European Pharmacopoeia which commonly use this strain throughout the pharmaceutical industry.

Pathogenicity
A. niger growing on onion

Plant pathogen

Aspergillus niger causes sooty mold on onions and ornamental plants. Infection of onion seedlings by A. niger can become systemic, manifesting only when conditions are conducive. A. niger causes a common postharvest disease of onions, in which the black conidia can be observed between scales of the bulb.

Human pathogen

A. niger has been found to cause physical manifestations in humans. Aspergillosis is a fungal infection caused by spores of indoor and outdoor Aspergillus mold species.[10] Due to the ubiquitous nature of A. niger, it's spores are commonly inhaled by humans from their surrounding environment.[11] However, aspergillosis infection customarily occurs in people with compromised immune systems or pre-existing lung conditions like asthma and cystic fibrosis.[10] Types of aspergillosis include allergic bronchopulmonary aspergillosis (ABPA), allergic aspergillus sinusitis, azole-resistant aspergillus fumigatus, cutaneous (skin) aspergillosis, and chronic pulmonary aspergillosis.[10] Out of the approximated 180 species of aspergillus molds, roughly 40 species have been found to cause health concern in immunocompromised humans.[10] Aspergillosis is particularly frequent among horticultural workers who often inhale peat dust, which can be rich in Aspergillus niger spores. The fungus has also been found in ancient Egyptian mummies and can be inhaled when they are disturbed.[12] Otomycosis, which is a superficial fungal infection of the ear canal, is another disorder that can be caused by overgrowth of Aspergillus molds like A. niger.[13] Otomycosis caused by A. niger is frequently associated with mechanical damage of the ear canal's external skin barrier and often presents itself in patients living in tropical climates.[13][14] A. niger is rarely reported to cause pneumonia compared to other Aspergillus species, such as Aspergillus flavus, Aspergillus fumigatus, and Aspergillus terreus.[15]

A. niger growing on PDA

Cultivation

A. niger is most commonly grown on Potato Dextrose Agar (PDA). However, it can grow on several types of growth medium including Czapek-Dox Agar, Lignocellulose Agar, and more.

  • A. niger growing on a Czapek medium plate
  • A. niger growing on a MEAOX plate
  • A. niger under a Foldscope

Fermentation

There are two ways in which Aspergillus niger can be grown for industrial purposes: solid state fermentation (SSF) and submerged fermentation (SmF).[16] SSF uses a solid substrate with nutrients and minimal moisture to grow microorganisms. Nutrients such as nitrogen and carbon come from agricultural byproducts such as wheat bran, sugar pulp, rice husks, and corn flour.[17] SSF gives better yield of microbe products and is more cost effective than SmF due to using agricultural byproducts.[18] SSF is predominantly used over SmF.[18] In SmF, microbes are grown in a liquid medium inside large aseptic fermentation vessels[17][18]. These vessels are expensive pieces of equipment that provide more water for growth and allow for tight control of environmental factors, such as temperature and pH, that affects microbial growth[18].

Industrial uses

Aspergillus niger is cultured to facilitate the industrial production of many substances.[19] Various strains of A. niger are used in the industrial preparation of citric acid (E330) and gluconic acid (E574); therefore, they have been deemed acceptable for daily intake by the World Health Organization.[20] A. niger fermentation is "generally recognized as safe" (GRAS) by the United States Food and Drug Administration under the Federal Food, Drug, and Cosmetic Act.[21] A. niger is also being considered as a potential new source of natural food grade pigments.[22]

The production of citric acid (CA) is achieved by growing strains of A. niger in a nutrient rich medium that includes high concentrations of sugar and mineral salts and an acidic pH of 2.5-3.5.[23] Many microorganisms produce CA, but Aspergillus niger produces more than 1 million metric tons of CA annually via a fungal fermentation process.[24] CA is in high demand for applications such as the control of microorganism growth, food and beverage flavor enhancement, acidity manipulation, pharmaceuticals, etc.[25]

A. niger produces many useful enzymes for the catabolism of biopolymers in order to obtain nutrients from its environment.[26] The production of specific enzymes can be increased for industrial purposes.[27][26] For example, A. niger glucoamylase (P69328) is used in the production of high-fructose corn syrup and pectinases (GH28) are used in cider and wine clarification. Alpha-galactosidase (GH27), an enzyme that breaks down certain complex sugars, is a component of Beano and several other products that decrease flatulence.[28] Another use for A. niger within the biotechnology industry is in the production of magnetic isotope-containing variants of biological macromolecules for NMR analysis.[29] Aspergillus niger is also cultured for the extraction of the enzyme, glucose oxidase (P13006), used in the design of glucose biosensors, due to its high affinity for β-D-glucose.[30][31]

In the food industry, A. niger is also cultured to isolate the enzyme fructosyltransferase to produce fructooligosaccharides (FOS).[32] FOS are used to manufacture low-calorie and functional foods due to FOS characteristic ability to slow growth of pathogenic microorganisms in the intestines.[32][33] These foods have prebiotic fiber among other health promoting properties. A. niger is not the only organism to produce the enzyme fructosyltransferase, but it has been found to produce the enzyme at rates conducive to industrial production.[32][33]

Aspergillus niger can grow in gold-mining solutions containing cyano-metal complexes with gold, silver, copper, iron, and zinc. The fungus also plays a role in the solubilization of heavy-metal sulfides.[34] A. niger has also been shown to remediate acid mine drainage through biosorption of copper and manganese.[35] Alkali-treated A. niger binds to silver to 10% of dry weight. Silver biosorption occurs by stoichiometric exchange with Ca(II) and Mg(II) of the sorbent.

Genome

Aspergillus niger has a genome consisting of roughly 34 megabases (Mb) organized into eight chromosomes.[36] The DNA contains 10,785 genes which are transcribed and translated into 10,593 proteins.[36]

Genomic information
NCBI genome ID429
Ploidyhaploid
Genome size34 Mb
Number of chromosomes8

Two strains of A. niger have been sequenced. Strain CBS 513.88 produces enzymes used in industrial applications while strain ATCC 1015 is the wildtype strain of ATCC 11414 used to produce industrial citric acid (CA).[37][38][39] The A. niger ATCC 1015 genome was sequenced by the Joint Genome Institute in a collaboration with other institutions.[40] Completed sequences have been used to uncover orthologous genes and pathways involved in fungal metabolism, specifically the catabolism of monosaccharides.[41] The ability of A. niger to change its metabolism depending on the carbon sources and other nutrients present in its environment has enabled the microorganism to survive and be found in almost all ecosystems. Further research is being done to study these mechanisms for all fungi using the complete sequenced genome of A. niger.[41]

See also
  • Contamination control

References
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