Axolotl

The axolotl (/ˈæksəlɒtəl/; from Classical Nahuatl: āxōlōtl [aːˈʃoːloːtɬ] (listen)), Ambystoma mexicanum,[3] is a paedomorphic salamander closely related to the tiger salamander.[3][4][5] Axolotls are unusual among amphibians in that they reach adulthood without undergoing metamorphosis. Instead of taking to the land, adults remain aquatic and gilled. The species was originally found in several lakes underlying what is now Mexico City, such as Lake Xochimilco and Lake Chalco.[1] These lakes were drained by Spanish settlers after the conquest of the Aztec Empire, leading to the destruction of much of the axolotl’s natural habitat.

Axolotl
CITES Appendix II (CITES)[2]
Scientific classification
Kingdom: Animalia
Phylum: Chordata
Class: Amphibia
Order: Urodela
Family: Ambystomatidae
Genus: Ambystoma
Species:
A. mexicanum
Binomial name
Ambystoma mexicanum
(Shaw and Nodder, 1798)
Its distribution is marked in red.
Synonyms[3]
  • Gyrinus mexicanus Shaw and Nodder, 1798
  • Siren pisciformis Shaw, 1802
  • Siredon axolotl Wagler, 1830
  • Axolotes guttata Owen, 1844
  • Siredon Humboldtii Duméril, Bibron, and Duméril, 1854
  • Amblystoma weismanni Wiedersheim, 1879
  • Siredon edule Dugès, 1888

Axolotls should not be confused with the larval stage of the closely related tiger salamander (A. tigrinum), which are widespread in much of North America and occasionally become paedomorphic. Neither should they be confused with mudpuppies (Necturus spp.), fully aquatic salamanders from a different family that are not closely related to the axolotl but bear a superficial resemblance.[6]

As of 2020, wild axolotls were near extinction[7][8] due to urbanization in Mexico City and consequent water pollution, as well as the introduction of invasive species such as tilapia and perch. They are listed as critically endangered in the wild, with a decreasing population of around 50 to 1,000 adult individuals, by the International Union for Conservation of Nature and Natural Resources (IUCN) and are listed under Appendix II of the Convention on International Trade in Endangered Species (CITES).[2] Axolotls are used extensively in scientific research due to their ability to regenerate limbs, gills and parts of their eyes and brains.[9] Axolotls were also sold as food in Mexican markets and were a staple in the Aztec diet.[10]

Description
A captive leucistic axolotl
Face of a dark axolotl
Speckled form
Axolotl's gills (Ambystoma mexicanum)

A sexually mature adult axolotl, at age 18–27 months, ranges in length from 15 to 45 cm (6 to 18 in), although a size close to 23 cm (9 in) is most common and greater than 30 cm (12 in) is rare. Axolotls possess features typical of salamander larvae, including external gills and a caudal fin extending from behind the head to the vent.[11][12] External gills are usually lost when salamander species mature into adulthood, although the axolotl maintains this feature.[13] This is due to their neoteny evolution, where axolotls are much more aquatic than other salamander species.[14]

Their heads are wide, and their eyes are lidless. Their limbs are underdeveloped and possess long, thin digits. Males are identified by their swollen cloacae lined with papillae, while females are noticeable for their wider bodies full of eggs. Three pairs of external gill stalks (rami) originate behind their heads and are used to move oxygenated water. The external gill rami are lined with filaments (fimbriae) to increase surface area for gas exchange.[13] Four-gill slits lined with gill rakers are hidden underneath the external gills, which prevent food from entering and allow particles to filter through.

Axolotls have barely visible vestigial teeth, which develop during metamorphosis. The primary method of feeding is by suction, during which their rakers interlock to close the gill slits. External gills are used for respiration, although buccal pumping (gulping air from the surface) may also be used to provide oxygen to their lungs.[13] Buccal pumping can occur in a two-stroke manner that pumps air from the mouth to the lungs, and with four-stroke that reverses this pathway with compression forces.

Buccal pumping
Axolotls displaying variations in color

Axolotls have four pigmentation genes; when mutated they create different color variants. The normal wild-type animal is brown/tan with gold speckles and an olive undertone. The five more common mutant colors are leucistic (pale pink with black eyes), golden albino (golden with gold eyes), xanthic (grey with black eyes), albino (pale pink/white with red eyes) which is more common in axolotls than some other creatures, and melanoid (all black/dark blue with no gold speckling or olive tone).[15] In addition, there is wide individual variability in the size, frequency, and intensity of the gold speckling and at least one variant that develops a black and white piebald appearance on reaching maturity. Because pet breeders frequently cross the variant colors, double homozygous mutants are common in the pet trade, especially white/pink animals with pink eyes that are double homozygous mutants for both the albino and leucistic trait.[16] Axolotls also have some limited ability to alter their color to provide better camouflage by changing the relative size and thickness of their melanophores.[17]

Habitat and ecology
Lake Xochimilco, Mexico City (Amanecer en Xochimilco). The native habitat of axolotls is important to the study of preservation and conservation.
Wild form

The axolotl is native only to the freshwater of Lake Xochimilco and Lake Chalco in the Valley of Mexico. Lake Chalco no longer exists, having been drained as a flood control measure, and Lake Xochimilco remains a remnant of its former self, existing mainly as canals. The water temperature in Xochimilco rarely rises above 20 °C (68 °F), although it may fall to 6–7 °C (43–45 °F) in the winter, and perhaps lower.[18]

Surveys in 1998, 2003, and 2008 found 6,000, 1,000, and 100 axolotls per square kilometer in its Lake Xochimilco habitat, respectively.[19] A four-month-long search in 2013, however, turned up no surviving individuals in the wild. Just a month later, two wild ones were spotted in a network of canals leading from Xochimilco.[20]

The wild population has been put under heavy pressure by the growth of Mexico City. The axolotl is currently on the International Union for Conservation of Nature's annual Red List of threatened species. Non-native fish, such as African tilapia and Asian carp, have also recently been introduced to the waters. These new fish have been eating the axolotls' young, as well as their primary source of food.[21]

Axolotls are members of the tiger salamander, or Ambystoma tigrinum, species complex, along with all other Mexican species of Ambystoma. Their habitat is like that of most neotenic species—a high-altitude body of water surrounded by a risky terrestrial environment. These conditions are thought to favor neoteny. However, a terrestrial population of Mexican tiger salamanders occupies and breeds in the axolotl's habitat.

The axolotl is carnivorous, consuming small prey such as mollusks,[22] worms, insects, other arthropods,[22] and small fish in the wild. Axolotls locate food by smell, and will "snap" at any potential meal, sucking the food into their stomachs with vacuum force.[23]

Use as a model organism
See also: Model organism
Leucistic axolotl in captivity

Today, the axolotl is still used in research as a model organism, and large numbers are bred in captivity. They are especially easy to breed compared to other salamanders in their family, which are rarely captive-bred due to the demands of terrestrial life. One attractive feature for research is the large and easily manipulated embryo, which allows viewing of the full development of a vertebrate. Axolotls are used in heart defect studies due to the presence of a mutant gene that causes heart failure in embryos. Since the embryos survive almost to hatching with no heart function, the defect is very observable. The axolotl is also considered an ideal animal model for the study of neural tube closure due to the similarities between human and axolotl neural plate and tube formation; the axolotl's neural tube, unlike the frog's, is not hidden under a layer of superficial epithelium.[24] There are also mutations affecting other organ systems some of which are not well characterized and others that are.[25] The genetics of the color variants of the axolotl have also been widely studied.[16]

Regeneration

The feature of the axolotl that attracts most attention is its healing ability: the axolotl does not heal by scarring and is capable of the regeneration of entire lost appendages in a period of months, and, in certain cases, more vital structures, such as tail, limb, central nervous system, and tissues of the eye and heart.[26] They can even restore less vital parts of their brains. They can also readily accept transplants from other individuals, including eyes and parts of the brain—restoring these alien organs to full functionality. In some cases, axolotls have been known to repair a damaged limb, as well as regenerating an additional one, ending up with an extra appendage that makes them attractive to pet owners as a novelty. In metamorphosed individuals, however, the ability to regenerate is greatly diminished. The axolotl is therefore used as a model for the development of limbs in vertebrates.[27] There are three basic requirements for regeneration of the limb: the wound epithelium, nerve signaling, and the presence of cells from the different limb axes.[28] A wound epidermis is quickly formed by the cells to cover up the site of the wound. In the following days, the cells of the wound epidermis divide and grow quickly forming a blastema, which means the wound is ready to heal and undergo patterning to form the new limb.

It is believed that during limb generation, axolotls have a different system to regulate their internal macrophage level and suppress inflammation, as scarring prevents proper healing and regeneration.[29] However, this belief has been questioned by other studies.[30] Axolotl’s regenerative properties leave the species as the perfect model to study the process of stem cells and its own neoteny feature. Current research can record specific examples of these regenerative properties through tracking cell fates and behaviors, lineage tracing skin triploid cell grafts, pigmentation imaging, electroporation, tissue clearing and lineage tracing from dye labeling. The newer technologies of germline modification and transgenesis are better suited for live imaging the regenerative processes that occur for axolotls.[31]

Genome

The 32 billion base pair long sequence of the axolotl's genome was published in 2018 and was the largest animal genome completed at the time. It revealed species-specific genetic pathways that may be responsible for limb regeneration.[32] Although the axolotl genome is about 10 times as large as the human genome, it encodes a similar number of proteins, namely 23,251[32] (the human genome encodes about 20,000 proteins). The size difference is mostly explained by a large fraction of repetitive sequences, but such repeated elements also contribute to increased median intron sizes (22,759 bp) which are 13, 16 and 25 times that observed in human (1,750 bp), mouse (1,469 bp) and Tibetan frog (906 bp), respectively.[32]

Neoteny
Main article: Neoteny

When most amphibians are young, they live in water, and they use gills that can breathe in the water. When they become adults, they go through a process called metamorphosis, in which they lose their gills and start living on land. However, the axolotl is unusual in that it has a lack of thyroid stimulating hormone, which is needed for the thyroid to produce thyroxine in order for the axolotl to go through metamorphosis; therefore, it keeps its gills and lives in water all its life, even after it becomes an adult and is able to reproduce. Its body has the capacity to go through metamorphosis if given the necessary hormone, but axolotls do not produce it, and must be exposed to it from an external source,[33] after which an axolotl undergoes an artificially-induced metamorphosis and begins living on land. One method of artificial metamorphosis induction is through an injection of iodine, which is used in the production of thyroid hormones.

An axolotl undergoing metamorphosis experiences a number of physiological changes that help them adapt to life on land. These include increased muscle tone in limbs, the absorption of gills and fins into the body, the development of eyelids, and a reduction in the skin's permeability to water, allowing the axolotl to stay more easily hydrated when on land. The lungs of an axolotl, though present alongside gills after reaching non-metamorphosed adulthood, develop further during metamorphosis.[34]

An axolotl that has gone through metamorphosis resembles an adult plateau tiger salamander, though the axolotl differs in its longer toes. The process of artificially inducing metamorphosis can often result in death during or even following a successful attempt, and so casual hobbyists are generally discouraged from attempting to induce metamorphosis in pet axolotls.[34]

Neoteny is the term for reaching sexual maturity without undergoing metamorphosis.[35] Many other species within the axolotl's genus are also either entirely neotenic or have neotenic populations. Sirens and Necturus are other neotenic salamanders, although unlike axolotls, they cannot be induced to metamorphose by an injection of iodine or thyroxine hormone.

The genes responsible for neoteny in laboratory animals may have been identified; however, they are not linked in wild populations, suggesting artificial selection is the cause of complete neoteny in laboratory and pet axolotls.[36]

Six adult axolotls (including a leucistic specimen) were shipped from Mexico City to the Jardin des Plantes in Paris in 1863. Unaware of their neoteny, Auguste Duméril was surprised when, instead of the axolotl, he found in the vivarium a new species, similar to the salamander. This discovery was the starting point of research about neoteny. It is not certain that Ambystoma velasci specimens were not included in the original shipment. Vilem Laufberger in Prague used thyroid hormone injections to induce an axolotl to grow into a terrestrial adult salamander. The experiment was repeated by Englishman Julian Huxley, who was unaware the experiment had already been done, using ground thyroids.[37] Since then, experiments have been done often with injections of iodine or various thyroid hormones used to induce metamorphosis.[14]

Neoteny has been observed in all salamander families in which it seems to be a survival mechanism, in aquatic environments only of mountain and hill, with little food and, in particular, with little iodine. In this way, salamanders can reproduce and survive in the form of a smaller larval stage, which is aquatic and requires a lower quality and quantity of food compared to the big adult, which is terrestrial. If the salamander larvae ingest a sufficient amount of iodine, directly or indirectly through cannibalism, they quickly begin metamorphosis and transform into bigger terrestrial adults, with higher dietary requirements.[38] In fact, in some high mountain lakes there live dwarf forms of salmonids that are caused by deficiencies in food and, in particular, iodine, which causes cretinism and dwarfism due to hypothyroidism, as it does in humans.

Captive care
These axolotls at Vancouver Aquarium are leucistic, with less pigmentation than normal.

The axolotl is a popular exotic pet like its relative, the tiger salamander (Ambystoma tigrinum). As for all poikilothermic organisms, lower temperatures result in slower metabolism and a very unhealthily reduced appetite. Temperatures at approximately 16 °C (61 °F) to 18 °C (64 °F) are suggested for captive axolotls to ensure sufficient food intake; stress resulting from more than a day's exposure to lower temperatures may quickly lead to disease and death, and temperatures higher than 24 °C (75 °F) may lead to metabolic rate increase, also causing stress and eventually death.[39][40] Chlorine, commonly added to tapwater, is harmful to axolotls. A single axolotl typically requires a 150-litre (40-US-gallon) tank. Axolotls spend the majority of the time at the bottom of the tank.[41]

This animal was X-rayed several times as part of a research project over a period of two years. It was a normal healthy adult (26.3 cm; 159.5 gm) at the beginning of the project and lived several more years after the project ended.[42]

Salts, such as Holtfreter's solution, are often added to the water to prevent infection.[43]

In captivity, axolotls eat a variety of readily available foods, including trout and salmon pellets, frozen or live bloodworms, earthworms, and waxworms. Axolotls can also eat feeder fish, but care should be taken as fish may contain parasites.[44]

Substrates are another important consideration for captive axolotls, as axolotls (like other amphibians and reptiles) tend to ingest bedding material together with food[45] and are commonly prone to gastrointestinal obstruction and foreign body ingestion.[46] Some common substrates used for animal enclosures can be harmful for amphibians and reptiles. Gravel (common in aquarium use) should not be used, and is recommended that any sand consists of smooth particles with a grain size of under 1mm.[45] One guide to axolotl care for laboratories notes that bowel obstructions are a common cause of death, and recommends that no items with a diameter below 3 cm (or approximately the size of the animal's head) should be available to the animal.[47]

There is some evidence that axolotls might seek out appropriately-sized gravel for use as gastroliths[48] based on experiments conducted at the University of Manitoba axolotl colony,[49][50] but these studies are outdated and not conclusive. As there is no conclusive evidence pointing to gastrolith use, gravel should be avoided due to the high risk of impaction.[51]

Cultural significance

The species is named after the Aztec deity Xolotl, who transformed himself into an axolotl. They continue to play an outsized cultural role in Mexico, and have appeared in cartoons and murals.[52]

In 2020, it was announced that the axolotl will be featured on the new design for Mexico's 50-peso banknote, along with images of maize and chinampas.[53]

See also
  • Mudpuppies
  • Olm
  • Texas salamander
  • Texas blind salamander
  • Lake Patzcuaro salamander
  • Barred tiger salamander
  • Amphibious fish
  • Handfish
  • Regenerative biomedicine

References
  1. IUCN SSC Amphibian Specialist Group (2020). "Ambystoma mexicanum". IUCN Red List of Threatened Species. 2020: e.T1095A53947343. doi:10.2305/IUCN.UK.2020-3.RLTS.T1095A53947343.en. Retrieved 12 November 2021.
  2. "Appendices | CITES". cites.org. Retrieved 2022-01-14.
  3. Frost, Darrel R. (2018). "Ambystoma mexicanum (Shaw and Nodder, 1798)". Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History. Retrieved 10 August 2018.
  4. "Mexican Walking Fish, Axolotls Ambystoma mexicanum" (PDF). Archived from the original (PDF) on 15 March 2018.
  5. "Axolotols (Walking Fish)". Aquarium Online. Archived from the original on 10 April 2013. Retrieved 2013-09-12.
  6. Malacinski, George M. (Spring 1978). "The Mexican Axolotl, Ambystoma mexicanum: Its Biology and Developmental Genetics, and Its Autonomous Cell-Lethal Genes". American Zoologist. 18 (2): 195–206. doi:10.1093/icb/18.2.195.
  7. Matt Walker (2009-08-26). "Axolotl verges on wild extinction". BBC. Retrieved 2010-06-28.
  8. PetAquariums.com. "Are Axolotls Endangered? You Need To Be Careful…". PetAquariums.com. Retrieved 2021-06-26.
  9. Weird Creatures with Nick Baker (Television series). Dartmoor, England, UK: The Science Channel. 2009-11-11. Event occurs at 00:25.
  10. "Mythic Salamander Faces Crucial Test: Survival in the Wild". The New York Times. Retrieved 30 July 2015.
  11. San Francisco Examiner (San Francisco, California) 7 August 1887, page 9, authored by Yda Addis
  12. McIndoe, Rosemary; Smith, D. G. (1984), Seymour, Roger S. (ed.), "Functional morphology of gills in larval amphibians", Respiration and metabolism of embryonic vertebrates: Satellite Symposium of the 29th International Congress of Physiological Sciences, Sydney, Australia, 1983, Perspectives in vertebrate science, Dordrecht: Springer Netherlands, pp. 55–69, doi:10.1007/978-94-009-6536-2_4, ISBN 978-94-009-6536-2, retrieved 2021-05-13
  13. Kardong, Kenneth V (2019). Vertebrates: comparative anatomy, function, evolution. ISBN 978-1-259-70091-0. OCLC 1053847969.
  14. Safi, Rachid; Bertrand, Stéphanie; Marchand, Oriane; Duffraisse, Marilyne; de Luze, Amaury; Vanacker, Jean-Marc; Maraninchi, Marie; Margotat, Alain; Demeneix, Barbara; Laudet, Vincent (2004-02-01). "The Axolotl (Ambystoma mexicanum), a Neotenic Amphibian, Expresses Functional Thyroid Hormone Receptors". Endocrinology. 145 (2): 760–772. doi:10.1210/en.2003-0913. PMID 14576183.
  15. "18 Types of Axolotl Colors You Can Own (Axolotl Color Guide)". August 14, 2019.
  16. Frost, Sally K.; Briggs, Fran; Malacinski, George M. (1984). "A color atlas of pigment genes in the Mexican axolotl (Ambystoma mexicanum)". Differentiation. 26 (1–3): 182–188. doi:10.1111/j.1432-0436.1984.tb01393.x.
  17. Pietsch, Paul; Schneider, Carl W. (1985). "Vision and the skin camouflage reactions of Ambystoma larvae: the effects of eye transplants and brain lesions". Brain Research. 340 (1): 37–60. doi:10.1016/0006-8993(85)90772-3. PMID 4027646. S2CID 22723238.
  18. "Lake Xochimilco, Borough of Xochimilco in southern México City, 162 L • Biotope Aquarium". Biotope Aquarium. Retrieved 2021-04-30.
  19. Stevenson, M. (2014-01-28). "Mexico's 'water monster' may have disappeared". SFGate.com. Associated Press. Retrieved 2014-01-29.
  20. "Endangered 'water monster' Axolotl found in Mexico City lake". The Independent. 2014-02-24. Retrieved 2017-06-02.
  21. "Mexico City's 'water monster' nears extinction". November 2008. Archived from the original on 2011-07-23. Retrieved 2010-06-28.
  22. "Ambystoma mexicanum (Salamandra ajolote)".
  23. Wainwright, P. C.; Sanford, C. P.; Reilly, S. M.; Lauder, G. V. (1989). "Evolution of motor patterns: aquatic feeding in salamanders and ray-finned fishes". Brain, Behavior and Evolution. 34 (6): 329–341. doi:10.1159/000116519. PMID 2611639.
  24. Gordon, R. (1985). "A review of the theories of vertebrate neurulation and their relationship to the mechanics of neural tube birth defects". Journal of Embryology and Experimental Morphology. 89 (Supplement): 229–255. PMID 3913733.
  25. Armstrong, John B. (1985). "The axolotl mutants". Developmental Genetics. 6 (1): 1–25. doi:10.1002/dvg.1020060102.
  26. Caballero-Pérez, Juan; Espinal-Centeno, Annie; Falcon, Francisco; García-Ortega, Luis F.; Curiel-Quesada, Everardo; Cruz-Hernández, Andrés; Bako, Laszlo; Chen, Xuemei; Martínez, Octavio; Alberto Arteaga-Vázquez, Mario; Herrera-Estrella, Luis (January 2018). "Transcriptional landscapes of Axolotl (Ambystoma mexicanum)". Developmental Biology. 433 (2): 227–239. doi:10.1016/j.ydbio.2017.08.022. PMID 29291975.
  27. Roy, S; Gatien, S (November 2008). "Regeneration in axolotls: a model to aim for!". Experimental Gerontology. 43 (11): 968–73. doi:10.1016/j.exger.2008.09.003. PMID 18814845. S2CID 31199048.
  28. McCusker, C, 2020
  29. Goodwin, James W.; Pinto, Alexander R.; Rosenthal, Nadia A. (June 4, 2013). Olson, Eric N. (ed.). "Macrophages are required for adult salamander limb regeneration". Proceedings of the National Academy of Sciences of the United States of America. 110 (23): 9415–9420. Bibcode:2013PNAS..110.9415G. doi:10.1073/pnas.1300290110. PMC 3677454. PMID 23690624.
  30. Pedersen, Katherine; Rasmussen, Rikke Kongsgaard; Dittrich, Anita; Pedersen, Michael; Lauridsen, Henrik (April 17, 2020). "Modulating the immune response and the pericardial environment with LPS or prednisolone in the axolotl does not change the regenerative capacity of cryoinjured hearts". The FASEB Journal. 34 (S1): 1. doi:10.1096/fasebj.2020.34.s1.04015. S2CID 218792957. Retrieved December 12, 2020.
  31. Masselink, Wouter, and Elly M. Tanaka. “Toward Whole Tissue Imaging of Axolotl Regeneration.” Developmental Dynamics, vol. 250, no. 6, 2020, pp. 800–806., https://doi.org/10.1002/dvdy.282.
  32. Nowoshilow, Sergej; Schloissnig, Siegfried; Fei, Ji-Feng; Dahl, Andreas; Pang, Andy W. C.; Pippel, Martin; Winkler, Sylke; Hastie, Alex R.; Young, George (2018-01-24). "The axolotl genome and the evolution of key tissue formation regulators". Nature. 554 (7690): 50–55. Bibcode:2018Natur.554...50N. doi:10.1038/nature25458. ISSN 1476-4687. PMID 29364872.
  33. Demircan, Turan; Ovezmyradov, Guvanch; Yıldırım, Berna; Keskin, İlknur; İlhan, Ayşe Elif; Fesçioğlu, Ece Cana; Öztürk, Gürkan; Yıldırım, Süleyman (2018-07-20). "Experimentally induced metamorphosis in highly regenerative axolotl (Ambystoma mexicanum) under constant diet restructures microbiota". Scientific Reports. 8 (1): 10974. doi:10.1038/s41598-018-29373-y. PMC 6054665. PMID 30030457.
  34. "Axolotls - Metamorphosed & Tiger Salamanders". www.axolotl.org. Retrieved 2022-01-25.
  35. Ley, Willy (February 1968). "Epitaph for a Lonely Olm". For Your Information. Galaxy Science Fiction. pp. 95–104.
  36. Malacinski, George M. (1978-05-01). "The Mexican Axolotl, Ambystoma mexicanum: Its Biology and Developmental Genetics, and Its Autonomous Cell-lethal Genes". American Zoologist. 18 (2): 195–206. doi:10.1093/icb/18.2.195.
  37. Reiß, Christian; Olsson, Lennart; Hoßfeld, Uwe (2015). "The history of the oldest self-sustaining laboratory animal: 150 years of axolotl research". Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 324 (5): 393–404. doi:10.1002/jez.b.22617. ISSN 1552-5015. PMID 25920413.
  38. Venturi, S. (2004). "Iodine and Evolution. DIMI-Marche". Archived from the original on 4 March 2017. Retrieved 25 September 2020.
  39. "Axolotls – Requirements & Water Conditions in Captivity". axolotl.org. Retrieved 2016-03-14.
  40. "Caudata Culture Species Entry – Ambystoma mexicanum – Axolotl". www.caudata.org. Archived from the original on 2016-03-15. Retrieved 2016-03-14.
  41. Wiegert, Joshua. "Axolotls: Keeping a Water Monster".
  42. Kulbisky, Gordon P; Rickey, Daniel W; Reed, Martin H; Björklund, Natalie; Gordon, Richard (1999). "The axolotl as an animal model for the comparison of 3-D ultrasound with plain film radiography". Ultrasound in Medicine and Biology. 25 (6): 969–975. doi:10.1016/s0301-5629(99)00040-x. PMID 10461726.
  43. Clare, John P. "Health and Diseases". axolotl.org.
  44. Strecker, Angela L.; Campbell, Philip M.; Olden, Julian D. (2011). "The Aquarium Trade as an Invasion Pathway in the Pacific Northwest". Fisheries. 36 (2): 74–85. doi:10.1577/03632415.2011.10389070.
  45. Pough, F. H. (1992). "Recommendations for the Care of Amphibians and Reptiles in Academic Institutions". Washington, D.C.: National Academy Press.
  46. Clayton, Leigh Ann; Gore, Stacey R. (2007). "Amphibian Emergency Medicine". Veterinary Clinics of North America: Exotic Animal Practice. 10 (2): 587–620. doi:10.1016/j.cvex.2007.02.004. PMID 17577564.
  47. Gresens, Jill (2004). "An Introduction to the Mexican Axolotl (Ambystoma mexicanum)". Lab Animal. 33 (9): 41–47. doi:10.1038/laban1004-41. PMID 15457201. S2CID 33299160.
  48. Wings, O A review of gastrolith function with implications for fossil vertebrates and a revised classification Acta Palaeontologica Polonica 52 (1): 1–16
  49. Gordon, N, Gastroliths – How I Learned to Stop Worrying and Love Gravel.
  50. Björklund, N.K. (1993). Small is beautiful: economical axolotl colony maintenance with natural spawnings as if axolotls mattered. In: Handbook on Practical Methods. Ed.: G.M. Malacinski & S.T. Duhon. Bloomington, Department of Biology, Indiana University: 38–47.
  51. Loh, Richmond (2015-05-15). "Common Disease Conditions in Axolotls". Archived from the original on 2020-08-04. Retrieved 2022-01-21.
  52. "Mexico's axolotl, a cartoon hero and genetic marvel, fights for survival". Reuters. 2018-11-20. Retrieved 2022-08-16.
  53. "Mexican axolotl will be the new image of the 50 peso bill". The Yucatan Times. 2020-02-21. Retrieved 2020-03-04.
Wikimedia Commons has media related to Ambystoma mexicanum.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.