Parasitoid wasp

Many parasitoid wasps use larval Lepidoptera as hosts, but some groups parasitize different host life stages (egg, larva or nymph, pupa, adult) of nearly all other orders of insects, especially Coleoptera, Diptera, Hemiptera and other Hymenoptera. Some attack arthropods other than insects: for instance, the Pompilidae specialise in catching spiders: these are quick and dangerous prey, often as large as the wasp itself, but the spider wasp is quicker, swiftly stinging her prey to immobilise it. Adult female wasps of most species oviposit into their hosts' bodies or eggs. Some also inject a mix of secretory products that paralyse the host or protect the egg from the host's immune system; these include polydnaviruses, ovarian proteins, and venom. If a polydnavirus is included, it infects the nuclei of host hemocytes and other cells, causing symptoms that benefit the parasite.[4][5]

Apparently healthy moth caterpillar feeds, grows, and molts...

...but endoparasitic koinobiont wasp larvae eventually fill its body and kill it.

Host size is important for the development of the parasitoid, as the host is its entire food supply until it emerges as an adult; small hosts often produce smaller parasitoids.[6] Some species preferentially lay female eggs in larger hosts and male eggs in smaller hosts, as the reproductive capabilities of males are limited less severely by smaller adult body size.[7]

Hornworm with parasitic wasp cocoons

Some parasitoid wasps mark the host with chemical signals to show that an egg has been laid there. This may both deter rivals from ovipositing, and signal to itself that no further egg is needed in that host, effectively reducing the chances that offspring will have to compete for food and increasing the offspring's survival.[8][9]

Potter wasp (Eumeninae), an idiobiont, building mud nest; she will then provision it with paralysed insects, on which she lays her eggs; she then seals the nest and provides no further care for her young.

On or inside the host the parasitoid egg hatches into a larva or two or more larvae (polyembryony). Endoparasitoid eggs can absorb fluids from the host body and grow several times in size from when they were first laid before hatching. The first instar larvae is often highly mobile and may have strong mandibles or other structures to compete with other parasitoid larvae. The following instars are generally more grub-like. Parasitoid larvae have incomplete digestive systems with no rear opening. This prevents the hosts from being contaminated by their wastes. The larva feeds on the host's tissues until ready to pupate; by then the host is generally either dead or almost so. A meconium, or the accumulated wastes from the larva is cast out as the larva transitions to a prepupa.[10][11] Depending on its species, the parasitoid then may eat its way out of the host or remain in the more or less empty skin. In either case it then generally spins a cocoon and pupates.

As adults, parasitoid wasps feed primarily on nectar from flowers. Females of some species will also drink hemolymph from hosts to gain additional nutrients for egg production.[12]

Polydnavirus-wasp mutualism: the virus protects koinobiont wasp eggs and larvae from immune suppression by the host's hemocytes.

Polydnaviruses are a unique group of insect viruses that have a mutualistic relationship with some parasitic wasps. The polydnavirus replicates in the oviducts of an adult female parasitoid wasp. The wasp benefits from this relationship because the virus provides protection for the parasitic larvae inside the host, (i) by weakening the host's immune system and (ii) by altering the host's cells to be more beneficial to the parasite. The relationship between these viruses and the wasp is obligatory in the sense that all individuals are infected with the viruses; the virus has been incorporated in the wasp's genome and is inherited.[13][14][15]

Based on genetic and fossil analysis, parasitoidism has evolved only once in the Hymenoptera, during the Permian, leading to a single clade, the Apocrita. All parasitoid wasps are descended from this lineage, except for the Orussoidea which are parasitic but lack the wasp waist. The Apocrita emerged during the Jurassic.[25][26][27][28] The Aculeata, which includes bees, ants, and parasitoid spider wasps, evolved from within the Apocrita; it contains many families of parasitoids, though not the Ichneumonoidea, Cynipoidea, and Chalcidoidea. The Hymenoptera, Apocrita, and Aculeata are all clades, but since each of these contains non-parasitic species, the parasitoid wasps, formerly known as the Parasitica, do not form a clade on their own.[28][29] The common ancestor in which parasitoidism evolved lived approximately 247 million years ago and was previously believed to be an ectoparasitoid wood wasp that fed on wood-boring beetle larvae. Species similar in lifestyle and morphology to this ancestor still exist in the Ichneumonoidea.[30][31] However, recent molecular and morphological analysis suggests this ancestor was endophagous, meaning it fed from within its host.[28] A significant radiation of species in the Hymenoptera occurred shortly after the evolution of parasitoidy in the order and is thought to have been a result of it.[29][31] The evolution of a wasp waist, a constriction in the abdomen of the Apocrita, contributed to rapid diversification as it increased maneuverability of the ovipositor, the organ off the rear segment of the abdomen used to lay eggs.[32]

The phylogenetic tree gives a condensed overview of the positions of parasitoidal groups (boldface), amongst groups (italics) like the Vespidae which have secondarily abandoned the parasitoid habit. The approximate numbers of species estimated to be in these groups, often much larger than the number so far described, is shown in parentheses, with estimates for the most populous also shown in boldface, like "(150,000)". Not all species in these groups are parasitoidal: for example, some Cynipoidea are phytophagous.

Trissolcus (family Platygastridae) on Chinavia eggs

Housefly pupae killed by parasitoid wasp larvae (probably Pteromalidae). Each pupa has one hole through which a single adult wasp has emerged after feeding on the housefly larva.

The parasitoid wasps are paraphyletic since the ants, bees, and non-parasitic wasps such as the Vespidae are not included, and there are many members of mainly parasitoidal families which are not themselves parasitic. Listed are Hymenopteran families where most members have a parasitoid lifestyle.[33]

Encarsia formosa, an endoparasitic aphelinid wasp, bred commercially to control whitefly in greenhouses

Trioxys complanatus, (Aphidiinae) ovipositing into a spotted alfalfa aphid, a commercial pest in Australia.[lower-alpha 1]

Parasitoid wasps are considered beneficial as they naturally control the population of many pest insects. They are widely used commercially (alongside other parasitoids such as tachinid flies) for biological pest control, for which the most important groups are the ichneumonid wasps, which prey mainly on caterpillars of butterflies and moths; braconid wasps, which attack caterpillars and a wide range of other insects including greenfly; chalcidoid wasps, which parasitise eggs and larvae of greenfly, whitefly, cabbage caterpillars, and scale insects.[35]

One of the first parasitoid wasps to enter commercial use was Encarsia formosa, an endoparasitic aphelinid. It has been used to control whitefly in greenhouses since the 1920s. Use of the insect fell almost to nothing, replaced by chemical pesticides by the 1940s. Since the 1970s, usage has revived, with renewed usage in Europe and Russia.[36] In some countries, such as New Zealand, it is the primary biological control agent used to control greenhouse whiteflies, particularly on crops such as tomato, a particularly difficult plant for predators to establish on.[37]

Commercially, there are two types of rearing systems: short-term seasonal daily output with high production of parasitoids per day, and long-term year-round low daily output with a range in production of 4–1000 million female parasitoids per week, to meet demand for suitable parasitoids for different crops.[38]

Parasitoid wasps influenced the thinking of Charles Darwin.[lower-alpha 2] In an 1860 letter to the American naturalist Asa Gray, Darwin wrote: "I cannot persuade myself that a beneficent and omnipotent God would have designedly created parasitic wasps with the express intention of their feeding within the living bodies of Caterpillars."[2] The palaeontologist Donald Prothero notes that religiously-minded people of the Victorian era, including Darwin, were horrified by this instance of evident cruelty in nature, particularly noticeable in the Ichneumonidae.[40]