POLR2A

Subunidad RPB1 de la ARN polimerasa II
Estructuras disponibles
PDB	Buscar ortólogos: PDBe, RCSB Lista de códigos PDB 2GHQ , 2GHT , 2LTO , 3D9K , 3D9L , 3D9M , 3D9N , 3D9O , 3D9P Estructuras enzimáticas RCSB PDB, PDBe, PDBsum
Identificadores
Símbolos	POLR2A (HGNC: 9187) MGC75453; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1
Identificadores; externos	OMIM: 180660 ; HomoloGene: 721 ; ChEMBL: 1641353; EBI: POLR2A; GeneCards: Gen POLR2A; UniProt: POLR2A; Bases de datos de enzimas IntEnz: entrada en IntEnz; BRENDA: entrada en BRENDA; ExPASy: NiceZime view; KEGG: entrada en KEEG; PRIAM: perfil PRIAM; ExplorEnz: entrada en ExplorEnz; MetaCyc: vía metabólica
Número EC	2.7.7.7
Locus	Cr. 17 p13.1
	Referencias: AmiGO / QuickGO
Patrón de expresión de ARNm
	Más información
Ortólogos
Humano	Ratón
5430	20020
Véase HS	Véase MM
P24928	P08775
NM_000937	NM_009089
NP_000928	NP_033115
Cr. 17:; 7.39 – 7.42 Mb	Cr. 11:; 69.73 – 69.76 Mb
;

La subunidad RPB1 de la ARN polimerasa II (POLR2A) es una enzima codificada en humanos por el gen polr2A.

Esta proteína se corresponde con la subunidad de mayor tamaño de la ARN polimerasa II, la polimerasa responsable de sintetizar el ARNm en eucariotas. POLR2A contiene un dominio en el extremo C-terminal compuesto de repeticiones de un heptapéptido que es esencial en la actividad de la polimerasa. Estas repeticiones contienen residuos de serina y treonina que son fosforilados durante la transcripción de la polimerasa. Además, esta subunidad en combinación con otras subunidades de la polimerasa, conforman un dominio de unión a ADN de la polimerasa, una especie de surco en el cual entra la molécula de ADN para ser transcrita a ARN.[1]

Interacciones

La proteína POLR2A ha demostrado ser capaz de interaccionar con:

Regulador 1 de la elongación de la transcripción[2]
CREBBP[3]
BRCA1[4][5][6][7]
GTF2H4[5]
Proteína de unión a TATA[5]
PCAF[3]
GTF2F1[5][3]
SUPT5H[8][9]
TFIIB[5]
POLR2H[10]
POLR2L[10]
MED26[11]
Cdk8[3]
ZNF74[12]
TCEA1[13][14][15]
SMYD3[16]
CTDP1[5]
POLR2C[10]
PQBP1[17]
SMARCA2[18][19][3]
SND1[20]
MED21[5][3][21]
SMARCB1[18][19][3]
POLR2E[10]
SMARCA4[18][19][22]
TAF11[5]

Referencias

«Entrez Gene: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa».
Carty, S M; Goldstrohm A C, Suñé C, Garcia-Blanco M A, Greenleaf A L (Aug. de 2000). «Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase II». Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 97 (16): 9015-20. ISSN 0027-8424. PMID 10908677. doi:10.1073/pnas.160266597.
Cho, H; Orphanides G, Sun X, Yang X J, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep. de 1998). «A human RNA polymerase II complex containing factors that modify chromatin structure». Mol. Cell. Biol. (UNITED STATES) 18 (9): 5355-63. ISSN 0270-7306. PMID 9710619.
Krum, Susan A; Miranda Gustavo A, Lin Chenwei, Lane Timothy F (Dec. de 2003). «BRCA1 associates with processive RNA polymerase II». J. Biol. Chem. (United States) 278 (52): 52012-20. ISSN 0021-9258. PMID 14506230. doi:10.1074/jbc.M308418200.
Scully, R; Anderson S F, Chao D M, Wei W, Ye L, Young R A, Livingston D M, Parvin J D (mayo de 1997). «BRCA1 is a component of the RNA polymerase II holoenzyme». Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 94 (11): 5605-10. ISSN 0027-8424. PMID 9159119.
Chiba, N; Parvin J D (Oct. de 2001). «Redistribution of BRCA1 among four different protein complexes following replication blockage». J. Biol. Chem. (United States) 276 (42): 38549-54. ISSN 0021-9258. PMID 11504724. doi:10.1074/jbc.M105227200.
Krum, Susan A; Womack James E, Lane Timothy F (Sep. de 2003). «Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity». Oncogene (England) 22 (38): 6032-44. ISSN 0950-9232. PMID 12955082. doi:10.1038/sj.onc.1206515.
Kim, J B; Yamaguchi Y, Wada T, Handa H, Sharp P A (Sep. de 1999). «Tat-SF1 protein associates with RAP30 and human SPT5 proteins». Mol. Cell. Biol. (UNITED STATES) 19 (9): 5960-8. ISSN 0270-7306. PMID 10454543.
Wada, T; Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog G A, Winston F, Buratowski S, Handa H (Feb. de 1998). «DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs». Genes Dev. (UNITED STATES) 12 (3): 343-56. ISSN 0890-9369. PMID 9450929.
Acker, J; de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (Jul. de 1997). «Interactions between the human RNA polymerase II subunits». J. Biol. Chem. (UNITED STATES) 272 (27): 16815-21. ISSN 0021-9258. PMID 9201987.
Sato, Shigeo; Tomomori-Sato Chieri, Parmely Tari J, Florens Laurence, Zybailov Boris, Swanson Selene K, Banks Charles A S, Jin Jingji, Cai Yong, Washburn Michael P, Conaway Joan Weliky, Conaway Ronald C (Jun. de 2004). «A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology». Mol. Cell (United States) 14 (5): 685-91. ISSN 1097-2765. PMID 15175163. doi:10.1016/j.molcel.2004.05.006.
Grondin, B; Côté F, Bazinet M, Vincent M, Aubry M (Oct. de 1997). «Direct interaction of the KRAB/Cys2-His2 zinc finger protein ZNF74 with a hyperphosphorylated form of the RNA polymerase II largest subunit». J. Biol. Chem. (UNITED STATES) 272 (44): 27877-85. ISSN 0021-9258. PMID 9346935.
Kettenberger, Hubert; Armache Karim-Jean, Cramer Patrick (Aug. de 2003). «Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage». Cell (United States) 114 (3): 347-57. ISSN 0092-8674. PMID 12914690.
Archambault, J; Pan G, Dahmus G K, Cartier M, Marshall N, Zhang S, Dahmus M E, Greenblatt J (Oct. de 1998). «FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO». J. Biol. Chem. (UNITED STATES) 273 (42): 27593-601. ISSN 0021-9258. PMID 9765293.
Pan, G; Aso T; Greenblatt J (Sep. de 1997). «Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators». J. Biol. Chem. (UNITED STATES) 272 (39): 24563-71. ISSN 0021-9258. PMID 9305922.
Hamamoto, Ryuji; Furukawa Yoichi, Morita Masashi, Iimura Yuko, Silva Fabio Pittella, Li Meihua, Yagyu Ryuichiro, Nakamura Yusuke (Aug. de 2004). «SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells». Nat. Cell Biol. (England) 6 (8): 731-40. ISSN 1465-7392. PMID 15303093. doi:10.1038/ncb1151.
Okazawa, Hitoshi; Rich Tina, Chang Alex, Lin Xi, Waragai Masaaki, Kajikawa Masunori, Enokido Yasushi, Komuro Akihiko, Kato Seishi, Shibata Masao, Hatanaka Hiroshi, Mouradian M Maral, Sudol Marius, Kanazawa Ichiro (mayo de 2002). «Interaction between mutant ataxin-1 and PQBP-1 affects transcription and cell death». Neuron (United States) 34 (5): 701-13. ISSN 0896-6273. PMID 12062012.
Sif, S; Saurin A J, Imbalzano A N, Kingston R E (Mar. de 2001). «Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes». Genes Dev. (United States) 15 (5): 603-18. ISSN 0890-9369. PMID 11238380. doi:10.1101/gad.872801.
Wang, W; Côté J, Xue Y, Zhou S, Khavari P A, Biggar S R, Muchardt C, Kalpana G V, Goff S P, Yaniv M, Workman J L, Crabtree G R (Oct. de 1996). «Purification and biochemical heterogeneity of the mammalian SWI-SNF complex». EMBO J. (ENGLAND) 15 (19): 5370-82. ISSN 0261-4189. PMID 8895581.
Yang, Jie; Aittomäki Saara, Pesu Marko, Carter Kara, Saarinen Jussi, Kalkkinen Nisse, Kieff Elliott, Silvennoinen Olli (Sep. de 2002). «Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II». EMBO J. (England) 21 (18): 4950-8. ISSN 0261-4189. PMID 12234934.
Suñé, C; Hayashi T, Liu Y, Lane W S, Young R A, Garcia-Blanco M A (Oct. de 1997). «CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription». Mol. Cell. Biol. (UNITED STATES) 17 (10): 6029-39. ISSN 0270-7306. PMID 9315662.
Zhao, K; Wang W, Rando O J, Xue Y, Swiderek K, Kuo A, Crabtree G R (Nov. de 1998). «Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling». Cell (UNITED STATES) 95 (5): 625-36. ISSN 0092-8674. PMID 9845365.

Datos: Q15335011

Este artículo ha sido escrito por Wikipedia. El texto está disponible bajo la licencia Creative Commons - Atribución - CompartirIgual. Pueden aplicarse cláusulas adicionales a los archivos multimedia.

[1] «Entrez Gene: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa».

[pmid10908677-2] Carty, S M; Goldstrohm A C, Suñé C, Garcia-Blanco M A, Greenleaf A L (Aug. de 2000). «Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase II». Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 97 (16): 9015-20. ISSN 0027-8424. PMID 10908677. doi:10.1073/pnas.160266597.

[pmid9710619-3] Cho, H; Orphanides G, Sun X, Yang X J, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep. de 1998). «A human RNA polymerase II complex containing factors that modify chromatin structure». Mol. Cell. Biol. (UNITED STATES) 18 (9): 5355-63. ISSN 0270-7306. PMID 9710619.

[pmid14506230-4] Krum, Susan A; Miranda Gustavo A, Lin Chenwei, Lane Timothy F (Dec. de 2003). «BRCA1 associates with processive RNA polymerase II». J. Biol. Chem. (United States) 278 (52): 52012-20. ISSN 0021-9258. PMID 14506230. doi:10.1074/jbc.M308418200.

[pmid9159119-5] Scully, R; Anderson S F, Chao D M, Wei W, Ye L, Young R A, Livingston D M, Parvin J D (mayo de 1997). «BRCA1 is a component of the RNA polymerase II holoenzyme». Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 94 (11): 5605-10. ISSN 0027-8424. PMID 9159119.

[pmid11504724-6] Chiba, N; Parvin J D (Oct. de 2001). «Redistribution of BRCA1 among four different protein complexes following replication blockage». J. Biol. Chem. (United States) 276 (42): 38549-54. ISSN 0021-9258. PMID 11504724. doi:10.1074/jbc.M105227200.

[pmid12955082-7] Krum, Susan A; Womack James E, Lane Timothy F (Sep. de 2003). «Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity». Oncogene (England) 22 (38): 6032-44. ISSN 0950-9232. PMID 12955082. doi:10.1038/sj.onc.1206515.

[pmid10454543-8] Kim, J B; Yamaguchi Y, Wada T, Handa H, Sharp P A (Sep. de 1999). «Tat-SF1 protein associates with RAP30 and human SPT5 proteins». Mol. Cell. Biol. (UNITED STATES) 19 (9): 5960-8. ISSN 0270-7306. PMID 10454543.

[pmid9450929-9] Wada, T; Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog G A, Winston F, Buratowski S, Handa H (Feb. de 1998). «DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs». Genes Dev. (UNITED STATES) 12 (3): 343-56. ISSN 0890-9369. PMID 9450929.

[pmid9201987-10] Acker, J; de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (Jul. de 1997). «Interactions between the human RNA polymerase II subunits». J. Biol. Chem. (UNITED STATES) 272 (27): 16815-21. ISSN 0021-9258. PMID 9201987.

[pmid15175163-11] Sato, Shigeo; Tomomori-Sato Chieri, Parmely Tari J, Florens Laurence, Zybailov Boris, Swanson Selene K, Banks Charles A S, Jin Jingji, Cai Yong, Washburn Michael P, Conaway Joan Weliky, Conaway Ronald C (Jun. de 2004). «A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology». Mol. Cell (United States) 14 (5): 685-91. ISSN 1097-2765. PMID 15175163. doi:10.1016/j.molcel.2004.05.006.

[pmid9346935-12] Grondin, B; Côté F, Bazinet M, Vincent M, Aubry M (Oct. de 1997). «Direct interaction of the KRAB/Cys2-His2 zinc finger protein ZNF74 with a hyperphosphorylated form of the RNA polymerase II largest subunit». J. Biol. Chem. (UNITED STATES) 272 (44): 27877-85. ISSN 0021-9258. PMID 9346935.

[pmid12914699-13] Kettenberger, Hubert; Armache Karim-Jean, Cramer Patrick (Aug. de 2003). «Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage». Cell (United States) 114 (3): 347-57. ISSN 0092-8674. PMID 12914690.

[pmid9765293-14] Archambault, J; Pan G, Dahmus G K, Cartier M, Marshall N, Zhang S, Dahmus M E, Greenblatt J (Oct. de 1998). «FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO». J. Biol. Chem. (UNITED STATES) 273 (42): 27593-601. ISSN 0021-9258. PMID 9765293.

[pmid9305922-15] Pan, G; Aso T; Greenblatt J (Sep. de 1997). «Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators». J. Biol. Chem. (UNITED STATES) 272 (39): 24563-71. ISSN 0021-9258. PMID 9305922.

[pmid15235609-16] Hamamoto, Ryuji; Furukawa Yoichi, Morita Masashi, Iimura Yuko, Silva Fabio Pittella, Li Meihua, Yagyu Ryuichiro, Nakamura Yusuke (Aug. de 2004). «SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells». Nat. Cell Biol. (England) 6 (8): 731-40. ISSN 1465-7392. PMID 15303093. doi:10.1038/ncb1151.

[pmid12062018-17] Okazawa, Hitoshi; Rich Tina, Chang Alex, Lin Xi, Waragai Masaaki, Kajikawa Masunori, Enokido Yasushi, Komuro Akihiko, Kato Seishi, Shibata Masao, Hatanaka Hiroshi, Mouradian M Maral, Sudol Marius, Kanazawa Ichiro (mayo de 2002). «Interaction between mutant ataxin-1 and PQBP-1 affects transcription and cell death». Neuron (United States) 34 (5): 701-13. ISSN 0896-6273. PMID 12062012.

[pmid11238380-18] Sif, S; Saurin A J, Imbalzano A N, Kingston R E (Mar. de 2001). «Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes». Genes Dev. (United States) 15 (5): 603-18. ISSN 0890-9369. PMID 11238380. doi:10.1101/gad.872801.

[pmid8895581-19] Wang, W; Côté J, Xue Y, Zhou S, Khavari P A, Biggar S R, Muchardt C, Kalpana G V, Goff S P, Yaniv M, Workman J L, Crabtree G R (Oct. de 1996). «Purification and biochemical heterogeneity of the mammalian SWI-SNF complex». EMBO J. (ENGLAND) 15 (19): 5370-82. ISSN 0261-4189. PMID 8895581.

[pmid12234934-20] Yang, Jie; Aittomäki Saara, Pesu Marko, Carter Kara, Saarinen Jussi, Kalkkinen Nisse, Kieff Elliott, Silvennoinen Olli (Sep. de 2002). «Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II». EMBO J. (England) 21 (18): 4950-8. ISSN 0261-4189. PMID 12234934.

[pmid9315662-21] Suñé, C; Hayashi T, Liu Y, Lane W S, Young R A, Garcia-Blanco M A (Oct. de 1997). «CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription». Mol. Cell. Biol. (UNITED STATES) 17 (10): 6029-39. ISSN 0270-7306. PMID 9315662.

[pmid9845365-22] Zhao, K; Wang W, Rando O J, Xue Y, Swiderek K, Kuo A, Crabtree G R (Nov. de 1998). «Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling». Cell (UNITED STATES) 95 (5): 625-36. ISSN 0092-8674. PMID 9845365.