Examples of Spectroscopy in the following topics:
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- This type of instrument is used in spectroscopy.
- Spectroscopy studies the interaction between matter and radiated energy.
- When the spectrometer produces a reading, the observer can then use spectroscopy to identify the atoms and therefore molecules that make up that object.
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- Structural determination using isotopes is often performed using nuclear magnetic resonance spectroscopy and mass spectrometry.
- Structural determination utilizing isotopes is often performed using two analytical techniques: nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS).
- Mass spectrometry and nuclear magnetic resonance detect the difference in an isotope's mass, while infrared spectroscopy detects the difference in the isotope's vibrational modes.
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- The power and usefulness of 1H nmr spectroscopy as a tool for structural analysis should be evident from the past discussion.
- The most important operational technique that has led to successful and routine 13C nmr spectroscopy is the use of high-field pulse technology coupled with broad-band heteronuclear decoupling of all protons.
- Unlike proton nmr spectroscopy, the relative strength of carbon nmr signals are not normally proportional to the number of atoms generating each one.
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- Carboxylic acids can be characterized by IR spectroscopy; they exhibit a sharp band associated with vibration of the C-O bond between 1680 and 1725 cm-1.
- By 1H NMR spectroscopy, the hydroxyl hydrogen appears in the 10–13 ppm region, although it is often either broadened or not observed owing to exchange with traces of water.
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- Over the past fifty years nuclear magnetic resonance spectroscopy, commonly referred to as nmr, has become the preeminent technique for determining the structure of organic compounds.
- Although larger amounts of sample are needed than for mass spectroscopy, nmr is non-destructive, and with modern instruments good data may be obtained from samples weighing less than a milligram.
- Strong magnetic fields are necessary for nmr spectroscopy.
- Nmr spectroscopy is therefore the energetically mildest probe used to examine the structure of molecules.
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- Just as a proton (spin = 1/2) will occupy one of two energy states in a strong external magnetic field, giving rise to nmr spectroscopy; an electron (spin = 1/2) may also assume two energy states in an external field.
- This complexity is the result of hyperfine splitting of the resonance signal by protons and other nuclear spins, an interaction similar to spin-spin splitting in nmr spectroscopy.
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- Each element's emission spectrum is unique, and therefore spectroscopy can be used to identify elements present in matter of unknown composition.
- Further series for hydrogen as well as other elements were discovered as spectroscopy techniques developed.
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- Consequently, absorption spectroscopy carried out in this region is sometimes called "electronic spectroscopy".
- The presence of chromophores in a molecule is best documented by UV-Visible spectroscopy, but the failure of most instruments to provide absorption data for wavelengths below 200 nm makes the detection of isolated chromophores problematic.
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