culture

Examples of culture in the following topics:

• Pure Culture

  • It is often essential to isolate a pure culture of microorganisms.
  • Another method of bacterial culture is liquid culture, in which the desired bacteria are suspended in liquid broth, a nutrient medium.
  • As an alternative, the microbiologist may decide to use static liquid cultures.
  • Geomyces destructans in culture from bat tissues.
  • Describe how pure microbial cultures can be grown in agar-based growth medium

• Tissue Culture of Animal Viruses

  • In practice, the term "cell culture" now refers to the culturing of cells derived from multi-cellular eukaryotes, especially animal cells.
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.
  • Cultured cells, eggs, and laboratory animals may be used for virus isolation.
  • Cell cultures vary greatly in their susceptibility to different viruses.
  • Discover the use of, and reasons for, culturing animal viruses in host cells

• Batch Culture of Bacteriophages

  • Bacteriophage cultures require host cells in which the virus or phage multiply.
  • Virus or phage cultures require host cells in which to multiply.
  • For bacteriophages, cultures are grown by infecting bacterial cells .
  • Virus or phage cultures require host cells in which to multiply.
  • For bacteriophages, cultures are grown by infecting bacterial cells.

• Preserving Bacterial Cultures

  • Take the overnight culture and and mix an aliquot with 40% glycerol in sterile water and place in a cryogenic vial.
  • While it is possible to make a long term stock from cells in the stationary phase, ideally your culture should be in logarithmic growth phase.
  • To do this, spin the culture down and resuspend it in the same volume of straight LB medium.
  • An erlenmeyer containing a bacterial culture.
  • Describe how bacterial cultures can be stored for a long time at -80C in glycerol

• Koch and Pure Culture

  • He invented methods to purify the bacillus from blood samples and grow pure cultures.
  • Additionally, it must be absent in healthy organisms prepared and maintained in a pure culture capable of producing the original infection, even after several generations in culture retrievable from an inoculated animal and cultured again.
  • Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture.
  • This is a useful technique for pure culture of fungi, multicellular algae, and small metazoa.
  • Developing pure culture techniques is crucial to the observation of the specimen in question.

• Special Culture Techniques

  • Animals can often be used to culture microbes.
  • Using animals to culture human-pathogens has problems.
  • Some human pathogens are grown directly on cells cultured from humans.
  • The human cell culture known as McCoy cell culture is used to culture this bacteria .
  • A candle is lit in a jar with a culture plate.

• Cultivation of Specimen

  • It is also critical that the pathogen is isolated in a pure culture first.
  • A patient's blood is usually cultured in a special bottle of broth that can be periodically sampled for growth.
  • Work must be done from isolated colonies or pure cultures, as working with mixed or contaminated cultures gives misleading and inaccurate results.
  • From such isolates, clinical microbiologists obtain information about a pathogen's microscopic morphology and staining reactions, culture appearance, motility, oxygen requirements, and biochemical characteristics.
  • This blood is cultured in a bottle to detect bloodstream infections.

• Limitation of Microbial Growth by Nutrient Supply

  • Nutrients are necessary for microbial growth and play a vital role in culturing microorganisms outside of their natural environment.
  • Hence, it is critical to identify the required nutrients and ensure these are supplied in the culturing of microorganisms.
  • An image of an anthrax culture grown on a petri dish.
  • In order for microogranisms to be cultured in the laboratory or undergo successful growth in their natural environment, the proper nutrients are absolutely necessary.
  • Describe the role of nutrients in microbial growth and their culture in the lab

• Diagnosing Microbial Diseases

  • The first tool in diagnosing microbial disease is microbial cultures.
  • It is critical to isolate the infectious agent in a pure culture containing only the infectious bacteria.
  • The use of microbial cultures is common to help in the clinical identification of pathogenic microbes.
  • However, there are specific classes of microbe that require culture within live animals.
  • The bacteria Mycobacterium leprae is such a microbe, as it can only be cultured in animals.

• Measurements of Microbial Mass

  • Cell cultures are turbid: they absorb some of the light and let the rest of it pass through.
  • The culture is placed in a translucent cuvette; the cuvette is placed in the machine and the turbidity measured immediately.
  • Using spectrophotometry for measuring the turbidity of cultures is known as turbidometry.
  • Additionally, there are spectrophotometers that require extremely small volumes of culture, as little as 1 microliter .
  • This, combined with the stochastic nature of liquid cultures, enables only an estimation of cell numbers.