polyadenylation

Examples of polyadenylation in the following topics:

• Vectors for Genomic Cloning and Sequencing

  • These plasmid transcription vectors characteristically lack crucial sequences that code for polyadenylation sequences and translation termination sequences in translated mRNAs, making protein expression from transcription vectors impossible.

• Unsticking Stuck Ribosomes

  • Stuck mRNA transcripts can arise from many different mechanisms such as premature 3' adenylation or cryptic polyadenylation signals within the coding region of a gene.

• Prions

  • The endogenous, properly-folded form is denoted PrPC (for Common or Cellular) while the disease-linked, misfolded form is denoted PrPSc (for Scrapie, after one of the diseases first linked to prions and neurodegeneration. ) The precise structure of the prion is not known, though they can be formed by combining PrPC, polyadenylic acid, and lipids in a Protein Misfolding Cyclic Amplification (PMCA) reaction.

• Plasmids as Cloning Vectors

  • A polyadenylation tail at the end of the transcribed pre-mRNA: This protects the mRNA from exonucleases and ensures transcriptional and translational termination and stabilizes mRNA production.