streak plate

(noun)

A petri dish with a growth medium.

Related Terms

Examples of streak plate in the following topics:

  • Direct Counting

    • One can also quantify the number of cells in a culture by plating a known volume of the cell culture on a petri dish with a growth medium, which is also known as a streak plate.
    • If the cells are distributed on the plate properly, it can generally be assumed that each cell will give rise to a single colony.
    • The colonies can then be counted and, based on the known volume of the culture that was spread on the plate, the cell concentration can be calculated.
    • As with hemocytometers or counting chambers, cultures need to be heavily diluted prior to plating.
    • Additionally, plating is the slowest method because most microorganisms need at least 12 hours to form visible colonies.

  • Aseptic Technique, Dilution, Streaking, and Spread Plates

    • Microbiologists rely on aseptic technique, dilution, colony streaking and spread plates for day-to-day experiments.
    • Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop.
    • Spread plates are simply microbes spread on a media plate.
    • Four streak plates.
    • Successful streaks lead to individual colonies of microbes.

  • Diagnosing Microbial Diseases

    • The most common method to isolate individual cells and produce a pure culture is to prepare a streak plate.
    • The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate.
    • This represents four nutrient agar plates with various bacterial species represented.
    • The use of plates for microbial culture aid in identification of microbes based on size, shape, colony formation and nutrient requirement.

  • Koch and Pure Culture

    • The most common method to isolate individual microbes and produce a pure culture is to prepare a streak plate.
    • The streak plate method is a way to physically separate the microbial population and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate.

  • Group B Streptococcus Colonization

    • Sometimes, before plating, enrichment of the gathered probe is performed.
    • The collected sample is streaked on a blood agar plate (vertical streaks) next to staphylococcus aureus culture (horizontal streak).
    • Strep B has weak hemolytic activity, which is enhanced substantially (arrow-like area) when streaked next to s. aureus.

  • Industrial Production of Antibiotics

  • Viable Cell Counting

    • Plate counting is used to estimate the number of viable cells that are present in a sample.
    • The plate count method or spread plate relies on bacteria growing a colony on a nutrient medium.
    • The colony becomes visible to the naked eye and the number of colonies on a plate can be counted.
    • Examples of a viable cell count are spread plates from a serial dilution of a liquid culture and pour plates.
    • Urine cultured on Oxoid Brilliance UTI Agar plate. 1uL of urine spread onto the agar surface.

  • Plant DNA Viruses

    • Maize streak virus is an insect-transmitted ssDNA maize virus in the genus Mastrevirus of the family Geminiviridae, endemic in sub-Saharan Africa.
    • It contains a wide range of plant viruses including bean golden mosaic virus, beet curly top virus, maize streak virus , and tomato pseudo-curly top virus, which together are responsible for a significant amount of crop damage worldwide.
    • The black-faced leafhopper (Graminella nigrifrons) transmits both maize fine streak virus and maize chlorotic dwarf virus.

  • Measurements of Microbial Mass

    • An additional method for the measurement of microbial mass is the quantification of cells in a culture by plating the cells on a petri dish.
    • If the cells are efficiently distributed on the plate, it can be generally assumed that each cell will give rise to a single colony.
    • The colonies can then be counted, and based on the known volume of culture that was spread on the plate the cell concentration can be calculated.
    • As is with counting chambers, cultures usually need to be heavily diluted prior to plating; otherwise, instead of obtaining single colonies that can be counted, a so-called "lawn" will form, resulting in thousands of colonies lying over each other.
    • Additionally, plating is the slowest method of all: most microorganisms need at least 12 hours to form visible colonies.

  • Bacterial Skin Diseases

    • Occasionally, a red streak extending to the lymph node can be seen.