Section 25
Molecular Techniques
By Boundless
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Transposons allow genes to be transferred to a host organism's chromosome, interrupting or modifying the function of a gene.
An insertion site is the position at which a transposable genetic element is integrated.
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Northern blots allow investigators to determine messenger RNA molecular weight and sample content.
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The Western blot technique determines protein molecular weight and measures protein abundance in different samples.
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DNA mobility shift assay is a technique for studying gene regulation and determining protein-DNA interactions.
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Protein tags are peptide sequences genetically grafted onto a recombinant protein.
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Primer extension is used to map the 5' ends of DNA or RNA fragments.
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DNA protection or "footprinting" analysis is a powerful technique for identifying the nucleotides involved in a protein-DNA interaction.
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Whole-genome DNA-binding analysis is a powerful tool for analyzing epigenetic modifications and DNA sequences bound to regulatory proteins.
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The two-hybrid method detects the interaction of two proteins by their ability to reconstitute the activity of a split transcription factor.