The grid is kept at 33 cm from the eye. The illumination of the chart should be good. Light exposure directly over the eye (in the form of slit-lamp examination, indirect ophthalmoscopy, and others) should be avoided immediately before using the grid to avoid a photostress effect. The patient wears near correction if any. Pupils should not be dilated. The patient is asked to close one eye. With the open eye through the near glasses, the patient is instructed to look at the central dot of the grid. In patients with poor fixation, the eye should focus at the center of the grid so that all the 4 corners are simultaneously visible. While keeping the eye fixated over the center of the grid, the patient has to appreciate is the lines are parallel or seem distorted (metamorphopsia). Also, he/she should note if some of the small squares are missing or blurry. The patient should mark the grid with the area of abnormality so that it can be compared later for any progression, stabilization, or improvement. If the patient can not see the corners, diseases like glaucoma and retinitis pigmentosa should be ruled out. Amsler grid should be checked at least once weekly.

 Alternative techniques to document metamorphopsia include:

• M chart[8] or metamorphopsia chart- There are 19 dotted lines with separation between the dots between 0.2° (fine) to 2° (coarse) visual angles. As the patient sees more coarsely separated dots, the metamorphopsia usually reduces and disappears. The metamorphopsia is quantified by 'the minimum visual angle of the dotted lines needed to cause the metamorphopsia to disappear'. This can measure both horizontal and vertical metamorphopsia. However, the shape and size of the metamorphopsia cannot be quantified using this test.
• Preferential hyperacuity perimeter PHP- Hyperacuity or Vernier acuity is a cortically mediated function to detect very subtle misalignments which may be as less as 3-6 seconds of arc. [9] It is defined as 'the ability to perceive a difference in the relative spatial localization of 2 or more visual stimuli'.[10]
  • Foresee Home (Notal Vision, Tel Aviv, Israel) is a type of PHP. It has been approved by the FDA (Food and Drug Administration, USA)  for 'use in the detection and characterization of central and paracentral metamorphopsia (visual distortion) in patients with age-related macular degeneration, as an aid in monitoring progression of disease factors causing metamorphopsia including but not limited to choroidal neovascularization (CNV). It is intended to be used at home for patients with stable fixation.' This device tests the central 14° of the visual field and total 500 data points are tested, 3-5 times each. However, it is not suitable for patients with cognitive decline (Alzheimer's disease) or if the patient can not establish a baseline in 2 attempts.
  • Macular Computerized Psychophysical Test (MCPT) uses hyperacuity to detect metamorphopsia.[11]
  • PreView PHP (Carl Zeiss Meditec, Dublin, CA)- This device also uses the principles of PHP. In a study, it was concluded that it can 'detect recent-onset CNV resulting from AMD and can differentiate it from an intermediate stage of AMD with high sensitivity and specificity'.[10]
• Shape discrimination hyperacuity (SDH)- Very minor sinusoidal deviation from circularity is detected by human eyes.[12] The threshold for SDH is <10 seconds of arc and is not affected by age or reduction of contrast at low radial frequencies.[9] Using this principle, radial shape discrimination testing may be used to detect new onset wet AMD.[13] Handheld SDH is available (myVisionTrack; Vital Art and Science, Inc., Richardson, TX) for smartphones (iOS; Apple, Inc., Cupertino, CA).[14] It has been cleared by FDA to be used as an Ophthalmic medical device,  'for the detection and characterization of central three degrees metamorphopsia (visual distortion) in patients with maculopathy, including age-related macular degeneration and diabetic eye disease, and as an aid in monitoring disease factors causing metamorphopsia. It is intended to be used by patients who have the capability to regularly perform a simple self-test at home'. The patient uses the software twice weekly and it notifies the eye care professional in case of any change in visual function.
• Differential perimetry[15] - In case of displacement of photoreceptors, the visual field projection changes. This leads to loss of correspondence of the visual field with the normal fellow eye. This can help document metamorphopsia using binocular perimetry with Goldmann perimeter.
• Three-dimensional computer-automated threshold Amsler grid test (3D CTAG)- This test has been described by Fink and Sadun from Doheny Eye Institute, California.[16] It characterizes the scotoma in 3 dimensions with excellent detail ('location, extent, slope, depth, and shape').[16]

Other techniques/instruments which may be used to document scotoma include-

• Perimeter- Static (Humphrey field analyzer, the 10-2 protocol for central scotoma) or dynamic (Goldmann perimeter, tangent screen), or both static and kinetic (Octopus)
• Microperimetry- This machine checks the visual threshold of a specific area of the retina so that a correlation between structure and function is possible in a pictorial manner on an overlay of the fundus image. It also identifies any preferred retinal loci. The available machines are Nidek MP-3 (Nidek Co. Ltd., Aichi, Japan), and Macular Integrity Assessment microperimeter (MAIA, CentreVue, Padova, Italy).[17]

Amsler grid is an important simple tool to detect or monitor macular diseases.

Metamorphopsia- Macropsia is denoted by the widening of the small squares compared to the nearby small squares. The parallel lines seem to be curved away from each other at this area. In the area of micropsia, the parallel lines seem to be drawn towards each other.

Scotoma- Merriam Webster dictionary defines scotoma as 'a spot in the visual field in which vision is absent or deficient.' A scotoma is surrounded by an area of the normal field of vision. A scotoma may be absolute (absent visual recognition of that area in spite of increasing the stimulus intensity) or relative (the stimulus presented at the scotoma becomes visible if stimulus intensity is increased). In Amsler grid, the relative scotoma appears as a veil partially obscuring the smaller squares and may be of different shapes. Such scotoma may be better appreciated if the illumination of the grid is reduced. Scotomas may be positive (something like spot or haze or smoke obscures central vision) or negative (the patient is unaware of scotoma unless tested).

Amsler grid home monitoring has various limitations including:

• Suprathreshold stimulus
• No monitor for fixation- stability of fixation is not monitored.
• No quantification of metamorphopsia
• Not interactive
• Scotoma detection and mapping may be suboptimal
• Need for good near vision to discern the grids
• Poor repeatability of scotoma[18]- Size, shape, and location of the scotoma detected on Amsler grid may not be same if the same patient is re-examined with Amsler grid even within 15 minutes. This may be related to 'perceptual completion phenomenon' which results from 'a filling in of the missing information when part of an image falls on a blind area of the visual field' which may be cortically mediated.[18][19] Due to the same phenomenon, the blind spot due to the optic disc is not usually appreciated. It has been noted that scotoma less than 6 degrees may not be detected by Amsler grid.[20]
• Low sensitivity for detection of peripheral changes due to the crowding effect of the peripheral lines
• Poor compliance

The modifications of the Amsler chart include:

• Threshold Amsler chart- The effective luminance of the white on black Amsler Grid may be lowered by placing a cross polarizing lens in front of the eye[21] and this may be better in picking up scotoma than the standard Amsler chart.[18]
• The sensitivity of the Amsler grid may also be improved with the use of red grids.[22]
• A Modified Amsler Chart with 'a grid of black lines on a white background measuring 12 × 12 cm with grid squares of 2 × 2 cm' has been reported by Shinoda and colleagues.[23] There are 6X6 or 36 squares. The patient with metamorphopsia is asked to trace the lines as they see it. The length of all the distorted lines drawn by the patient is measured in milimeters except the outer margin of the chart.
• Sine Amsler chart[24] uses 8 modified Amsler grids with central sine waves instead of lines. The frequency of the sine wave is constant in each chart, but the amplitude is different. The patient is asked to note the amount of metamorphopsia in an Amsler grid. Then he/she is asked to corroborate the magnitude of metamorphopsia to a specific sine Amsler chart
• Morphision[25]- It consists of 50 modified Amsler grids in an A5 sized booklet. 'The control plate (1) allows detection and localization of distortion, the second plate (2) identifies the pattern of distortion, and the remaining 48 plates quantify metamorphopsia'.[25] Various charts consist of sine waves replacing lines, and cushion or barrel-like distortion.

Appropriate use of the Amsler grid requires an interprofessional team. The nurses and optometrists who are involved in patient care should be taught how to handle the instrument properly without any damage. Also, the nurses should assist the clinician with giving proper instructions to the patient so that the final result will not be ambiguous. Often patients will use the Amsler grid at home. The nurse should monitor compliance and report to the ophthalmologist or optometrist if the patient is not compliant or inappropriately using the grid. It is also important to not to apply any mydriatic in the eye prior to the test. If the nurse or clinician accidentally uses a mydriatic, the team should be informed of the application and the test rescheduled on a later date. Interprofessional application and education of patients using the Amsler grid will result in the best outcomes. [Level V]