There are 2 distinct phases of the fascioliasis infection.

The acute (hepatic) phase usually begins 6 to 12 weeks after ingestion of metacercariae from a contaminated water source. The first sign is usually very high fever, followed by right upper quadrant pain, hepatomegaly, and occasionally jaundice. Cell blood count (CBC) differential will show a marked peripheral eosinophilia. Patients often present with associated myalgias, urticarial rash, nausea, anorexia, and diarrhea. These symptoms are attributed to the Fasciola flatworms migrating through the liver parenchyma and setting off the inflammatory and immune responses along the way. Additional laboratory diagnostic clues include transaminitis, anemia, elevated erythrocyte sedimentation rate (ESR), and hypergammaglobulinemia. Early complications are rare but may be seen with high parasite load and include ascites, hemobilia, subcapsular hematomas, and rarely severe parenchymal liver necrosis. However, in most cases, acute symptoms generally resolve within 6 weeks, and the infection settles into the chronic phase.[1]

Early extrahepatic manifestations are typically type III (immune complex deposition) or type IV (IgE) hypersensitivity-mediated. A reactive eosinophilic pneumonitis is nonspecific for helminth infections and is well described in literature.[9] Overwhelming activation of the host’s immune system and inflammatory response can cause vasculitis. In the heart can lead to myocarditis which in turn can cause cardiac conduction abnormalities. In the brain cerebral vasculitis may lead to focal neurologic deficits or seizures but these are all highly uncommon. Generalized lymphadenopathy is often present.[1]

The chronic (biliary) phase usually begins about 6 months after acute infection once the flukes have settled into the bile ducts and may last up to a decade or more. Usually asymptomatic, but occasionally can present as chronic epigastric and right upper quadrant pain, nausea, vomiting, diarrhea, hepatomegaly, jaundice, and failure to thrive. Peripheral eosinophilia will not likely be present. Chronic common bile duct obstructions may develop and lead to recurrent jaundice, cholelithiasis, pancreatitis, and more seriously, ascending cholangitis. Prolonged infection and/or high parasite load leads to chronic hepatobiliary damage and can result in chronic biliary cirrhosis, sclerosing cholangitis, and even cholangiocarcinoma.[1][10][11]

Fasciola’s migration out of the intestine and up into the liver is not always perfect; sometimes they get lost. Ectopic fascioliasis is the term given to the infection occurring outside the hepatobiliary system. They may end up almost anywhere in the body. Their presence results in localized mononuclear, eosinophilic infiltration that causes secondary tissue damage from the host’s immune response. Frequently, when they get lost, they end up in the subcutaneous tissue of the abdominal wall, but they may also end up in the peritoneum, intestinal wall, lungs, heart, brain, muscles, or eyes. Traveling flatworms under the skin or fat tissue leave behind migrating, pruritic, erythematous, painful nodules 1 to 6 cm in diameter and may be seen or palpated. Occasionally nodules can become infected and turn into a local abscess.[1][12][13][14][15] Another rare form of ectopic fascioliasis is Halzoun syndrome or pharyngeal fascioliasis. It is sometimes seen in the Middle East in areas where people eat raw liver. In this case, the metacercariae or young adult flukes living in the ingested liver attach themselves to the mucosa of the upper respiratory or digestive tract causing pharyngitis or esophagitis. Rarely edema and swelling of the upper airway can be so severe that patients have asphyxiated.[16]

Fascioliasis is exceedingly rare in the United States, but should be on the differential for any patient with the combination of abdominal pain (especially right upper quadrant), transaminitis, and marked peripheral eosinophilia. Even more suspicion should arise if a patient has traveled to endemic areas of Europe, Asia, or the Pacific, and their dietary history includes watercress ingestion or consumption of raw vegetables washed in potentially contaminated water. Often there is a delay in the diagnosis of this disease even in endemics areas due to its rarity and nonspecific acute symptoms.[2][17]

Serology has become the fastest and most efficient way to test for fascioliasis in recent years. Titers against fasciola antigens become positive during the early phase of parasite migration and are detectable 2 to 4 weeks following initial exposure to the host’s immune system. Enzyme-linked immunosorbent assay (ELISA) techniques have largely replaced stool ova and parasite testing because of its fast turn-around, sensitivity, and quantification.[18][19] Serum fasciola antigen levels often positively correlate with the degree of infection.[20] It also takes 5 to 7 weeks after initial infection before adult worms are mature enough to produce eggs; thus, there are no eggs in stool during the acute phase of the infection. Antigen levels positively correlate with the infectious burden. Successful treatment and extermination of the parasites correlate with a decline in the ELISA titers to fasciola antigens. Antibodies may still be detectable at low levels for years. The assay becomes undetectable in approximately 65% of patients one month following successful treatment. Some patients will have a low positive titer for life without any evidence of active infection.[21][22]

Microscopic examination of stool or duodenal bile aspirates for eggs is still useful for detecting chronic infections in rural or third world settings where resources can be scarce. They are also sometimes found incidentally while working up biliary obstructions. Fasciola hepatica eggs are yellow-brown and ovoid measuring 130 to 150 micrometers long by 60 to 90 micrometers wide. A single adult fluke can release over 20,000 eggs per day, but that release is intermittent. Thus, an examination of multiple stool or duodenal aspirate specimens may be needed as one negative examination does not necessarily exclude the diagnosis.[23]

Rarely, one might also identify adult flukes in the biliary tree during endoscopic retrograde cholangio-pancreatograhy (ERCP) during assessment in a patient with biliary obstruction. Occasionally capsular liver nodules can be seen by a surgeon during a diagnostic laparoscopy, but with no further clinical history are nonspecific. Liver biopsy is not routinely warranted as serology if much more specific, sensitive, and cost effective.[24]

Computerized Tomography scan may show multiple, nodular, small (approximately 25 mm in diameter), branching, subcapsular lesions in the parenchyma of the liver. These tortuous tracks are left behind by the migrating parasites. Necrotic areas may be seen on scans utilizing inravaenous (IV) contrast.[24][25][26] Subcapsular hematoma, capsular thickening, or parenchymal calcifications can also be seen.[27]

Ultrasound, cholangiogram, and ERCP are helpful during the biliary stage and may show the mobile, leaf-like flukes in biliary ducts or gallbladder, and are often associated with stones. Irregular common bile duct wall thickening, hepatomegaly, splenomegaly, and/or peri-portal lymphadenopathy are often present, especially in acute phase.[24]

The treatment of choice is oral triclabendazole 10 mg/kg daily taken after a meal for 2 days. Triclabendazole is an imidazole derivative and works by preventing the polymerization of tubulin into microtubules rendering cells incapable of producing their cytoskeletal structures. It is effective against all stages of fascioliasis with a cure rate of over 90%.[28][29] Fasciola species do not respond well to ivermectin, praziquantel, artesunate, mebendazole, or albendazole.[30] 

Acute hepatitis, autoimmune hepatitis, and shock liver will all have very significantly elevated transaminases, typically in the thousands. This is much high than the levels seen in fascioliasis which are usually in the hundreds.

Toxocariasis, acute schistosomiasis, ascariasis, and strongyloidiasis, are all nearly indistinguishable from fascioliasis in the acute phase, especially in the presence of peripheral eosinophilia and pulmonary symptoms. Serologic testing is required to rule these out.

Triclabendazole is effective against all stages of fascioliasis with a cure rate of over 9%.[28][29]

Chronic infections can be complicated by ascending cholangitis which requires IV antibiotics and usually surgery. Biliary obstruction by parasites in the setting of ascending cholangitis requires emergency endoscopic retrograde cholangiopancreatography (ERCP) for the direct removal of the worms from the common bile duct if possible. Chronic infections lead to chronic biliary inflammation and destruction giving rise to sclerosing cholangitis, biliary cirrhosis, and cholangiocarcinoma.[10][11][5]

Follow-up after treatment should assess for a decrease in serologic titers, resolution of eosinophilia, and clearance of eggs in the stool. It is reasonable to repeat all positive tests at 3 months post-treatment. Resolution of biliary findings on ultrasound after therapy may also be helpful if the patient presented with biliary obstruction as scar tissue and biliary duct thickening may still be present and can cause prolonged symptoms.[31]

When a patient is diagnosed with fascioliasis, other family or house members should be tested for serum fasciola antigens. Any patient who tests positive, regardless if they are symptomatic or not, should be treated to avoid the risk of future complications.

Infection can be prevented by educating patients to avoid ingestion of raw freshwater plants, especially in endemic areas. Elimination of snail intermediates have been attempted in endemic areas in the past but is not practical. There are no vaccines available for humans and the disease is much too rare in the human population for one to be economically beneficial.

Management of fascioliasis starts with early recognition of this disease that often goes undiagnosed. It requires a high degree of suspicion and understanding that serologic evaluation is the most beneficial and cost-effective analysis to assess for the disease.[17]

Coordination of care requires a team of healthcare providers that includes physicians, often primary care hospitalists along with gastroenterologists and often infectious disease specialists. Nurses, laboratory technicians, and pharmacists also play a part in the handling and evaluation of specimens and the delivery of the care to the patient.[28]