The lumbar puncture is generally performed by one person. A second person, typically, a nurse (RN), may assist with the procedure. The person performing the lumbar puncture and the assistant should both be in sterile gowns and observe sterile precautions throughout the procedure. 

As with any procedure, preparation is key to ensure safety and improve the likelihood of success. First, begin by assessing whether there are any contraindications to performing the procedure. Clinical signs of or risk factors for increased intracranial pressure, coagulopathy, or thrombocytopenia should be evaluated before performing a lumbar puncture. Review the indications listed for head CT before performing a lumbar puncture (LP).

Before beginning, the procedure, as well as risks and benefits, should be explained to the patient. Informed consent should be obtained before performing the procedure.[9]

The positioning of the patient in either a lateral recumbent position or sitting position may be used. The lateral recumbent position is preferred as it will allow an accurate measurement of opening pressure, and it also reduces the risk of post-lumbar puncture headache. The patient should be instructed to assume the fetal position, which involves the flexion of the spine. It may be helpful to instruct the patient to flex their back "like a cat." By doing so, the space between the spinous processes increases, allowing for easier needle insertion. To help keep the needle at the midline during insertion, the lumbar spine should be perpendicular to the table in the sitting position and parallel to the table if in the recumbent position. 

The ideal insertion point of the spinal needle should be either in the interspinous area between L4 and L5 or L3 and L4. Using these landmarks will avoid inadvertent damage to the conus medullaris, which typically terminates at L1. Palpation of landmarks along the back of the patient is used to locate the ideal insertion point for the needle. Certain conditions such as obesity, scoliosis, and degenerative disc disease may make palpation of landmarks more difficult. A line should be drawn between the superior aspects of the iliac crests. This line is referred to as the intercristal line or Tuffier line. This line typically intersects the spine along the L4 spinous process; however, this should only serve as a general reference as there are anatomical variations from person to person. The intercristal line may intersect at a higher level than L4 if the patient is female or obese.[10] Palpate the landmarks before cleaning the skin are and before administration of local anesthesia. Once the interspinous space is palpated, use a skin marking pen to mark the area of needle insertion.

The lumbar puncture is considered an aseptic procedure.[2] Dawn sterile gloves and gown and clean the area with a disinfecting agent. The cleaning agent should be applied to the skin in a widening concentric circular fashion starting at the point of needle insertion and circling outward. Allow the cleaned area to dry. Drape the area with sterile drapes. 

Prepare CSF collection vials in order by labeling them according to the priority of collection. Typically they are labeled 1 through 4. 

After the area has been cleansed and draped, apply a local anesthetic to the area of needle insertion. It is important to have marked the area of needle insertion before administration of local anesthetic as it may result in loss of landmarks.[11][12][13]

Identify the anatomical landmarks once again before insertion of the spinal needle. The spinal needle should be inserted with the stylet in place and with the bevel in the sagittal plane. The bevel should face the ceiling if the patient is in the lateral decubitus position. This will spread the fibers of the cauda equina instead of cutting them during insertion. The fibers of the cauda equina run parallel to the spinal axis. Insert the needle in the midline at the superior aspect of the inferior spinous process. If inserting the needle in the L4-L5 interspace, the needle should be inserted along the superior aspect of the L5 spinous process. The needle should be inserted at an angle of approximately 15 degrees. It may be helpful to imagine aiming the needle towards the patient's umbilicus. 

The needle should be inserted smoothly in a singular direction. There may be some minor resistance when passing through the ligamentum flavum, which may result in a "popping" sensation. After passing the ligamentum flavum, advance the needle in 2 mm increments each time removing the stylet to see if there is any CSF fluid draining. A standard Whitacre spinal needle will be inserted to approximately 2/3 depth before reaching the ligamentum flavum.[2] If a bone is encountered or there is no CSF fluid obtained, withdraw the needle to the subcutaneous tissue without exiting the skin, and redirect the needle. Do not redirect the needle while inserted beyond the subcutaneous tissue, as this will increase the risk of a traumatic lumbar puncture. A traumatic lumbar puncture will be tinged with blood. This typically occurs when the dorsal or epidural venous plexuses are pierced by the spinal needle.[14] If there is substantial blood present in the needle, it may become clogged. If this occurs, insert a new spinal needle at a different site. If unable to obtain CSF from the initial needle insertion site, an alternate site may be used. The use of ultrasound guidance has been shown to improve success rates.[15] An alternative technique known as the paramedian approach may be used to reach the subarachnoid space if the typical medial approach is unsuccessful. The point of needle entry is 1 cm inferior and 1 cm lateral to the typical L4 landmark. Needle insertion will be angled medially and cephalad at an angle of 15 degrees and advanced until CSF is obtained.[16][17]

CSF fluid will flow once the subarachnoid space has been reached by the needle. If obtaining an opening pressure, ensure the patient is in the lateral decubitus position to ensure the readings will be accurate.[18] Attach the manometer to the spinal needle by using a flexible connector. Allow the CSF fluid level to rise in the manometer. Document the level at which the CSF fluid stops rising. Pulsatile variations may be present with the patient's respirations. Normal opening pressure ranges between 6 cm of water to 25 cm of water.[18] After obtaining and documenting the opening pressure, disconnect the tubing. Allow the CSF to drain passively from the needle hub into the collection vials. Do not aspirate the CSF fluid. For most routine diagnostic studies, collect approximately 1 mL per collection vial. After the collection of CSF fluid samples, replace the stylet into the spinal needle, and remove the needle. Apply gentle pressure with sterile gauze over the area where the needle was inserted. Cover the area with a small bandage.

Dispose of all sharps in the appropriate containers.[11][12][13]

Understanding the potential risks and complications associated with a lumbar puncture is of great importance and should be discussed with the patient before performing the procedure. Serious complications are rare, and risk can be minimized if proper technique and precautions are followed. The complications of a lumbar puncture include post-lumbar puncture headache, bleeding, infection, spinal hematoma, and cerebral herniation.[19] The most common complication observed after a lumbar puncture is a post-lumbar puncture headache.[20] Removal of the spinal needle with the stylet in place has been shown to reduce the incidence of post lumbar puncture headache.[21] Use of an atraumatic spinal needle (pencil-point spinal needle) and a smaller gauge is associated with fewer post-lumbar headaches as compared to cutting needle.[3][20] 

Spinal hematoma is of particular concern in patients with coagulopathies or currently receiving certain anticoagulant medications.[14] If a spinal subarachnoid hematoma develops, the patient may complain of acute back pain or new neurologic symptoms. The diagnosis can be confirmed with a magnetic resonance imaging (MRI) scan of the brain.[14]

Lumbar puncture is one of the most commonly performed procedures in the emergency department. It is used in the diagnosis of potentially life-threatening diseases such as meningitis and subarachnoid hemorrhage. A thorough understanding of anatomy, potential complications, and various techniques helps to ensure a successful and safe lumbar puncture. Maintaining open communication with the patient throughout the procedure may decrease patient anxiety and assist in first attempt success. 

Lumbar puncture is a commonly performed procedure in the emergency department and can be of great clinical importance when diagnosing potentially lethal diseases such as meningitis and subarachnoid hemorrhage. With proper preparation, technique, and care, the risks of complications can be significantly reduced. Mental visualization of the anatomical landmarks before insertion of the spinal needle will aid in the successful completion of a lumbar puncture. Informed decision making and discussion of risk-benefit with the patient will help reduce patient anxiety and lead to better outcomes.