Antidiuretic Hormone (ADH)

ADH, also known as vasopressin, acts as a water preserving hormone. ADH is released into the blood circulation to vasoconstrict and reabsorbs water from the collecting duct in the kidney to maintain equilibrium intracellularly and extracellularly. In the hypothalamus, there are two regulating receptors that sense water depletion and hyperosmolar concentration, the subfornical organ, and organum vasculosum. This particular area of the nervous system does not have an intact blood-brain barrier. Therefore, blood products can come into contact with the structures with ease and eventually signal the hypothalamus for ADH secretion. A small concentration is enough to generate water conservation in the renal tubules. The renal tubules are broken down into segments known as the proximal, descending, ascending, distal, and collecting duct. The collecting duct is a highly diluted, ADH dependent structure in the kidney. The cells in the collecting duct have a 7 pass receptor on the basolateral side where ADH can bind itself. The signal unleashes a power generating cascade known as cAMP. The Gs protein stimulates adenylyl cyclase, which converts ATP into cAMP. High concentration of cAMP will phosphorylate protein kinase A, opening water channels to allow passage from the luminal side to the basolateral side. The channels are gated vesicles known as aquaporins. The amount of water absorbed from the collecting duct is dependent on the amount of fluid loss.[3][4][5]

Oxytocin

Oxytocin is a polypeptide hormone that is produced in the paraventricular nuclei for storage and release from the pituitary gland. It is commonly excreted in females during the birthing process. It functions to allow the uterus to contract, release milk from the breast and is present during ejaculation in males.[6]

Oxytocin: Uterus

When the fetus reaches an appropriate size, it stretches the uterine muscle lining, transmitting signals to the hypothalamus which generates and releases oxytocin into the systemic circulation. The hormone binds to the myometrial layer of the uterus where the smooth muscle is present. Once oxytocin attaches to the extracellular receptor, it activates the Gq protein, which then activates another cell membrane protein called Phospholipase C. PLC will break down phosphoinositol diphosphate in two components, diacylglycerol (DAG)  and Inositol triphosphate (IP3). IP3 will push calcium from the sarcoplasmic reticulum while DAG activates protein kinase C. This protein will phosphorylate proteins on the cell membrane to specifically allow calcium entry from extracellular space. An increase in intracellular calcium will generate enough energy to cause contraction of the uterus advancing the fetus into the vagina for delivery.

 Oxytocin: Lactation

The regulation of lactation is similar to uterine contraction where an external source stimulates the production of oxytocin by the hypothalamus. During lactation, the newborn, by means of “suckling”, will transmit a signal to the central nervous system. This process is known as the “milk letdown reflex.” Once oxytocin binds to myoepithelial cell receptors in the breast it will undergo the same Gq cascade as uterine contraction, ejecting milk forward into the baby’s oral cavity.

Oxytocin: Ejaculation

A similar concept holds for sexual orgasmic response in males. During ejaculation, the oxytocin contracts the vas deferens to push the sperm and semen forward for ejection.

Hyposecretion

Central Diabetes Insipidus (CDI)

CDI is the most common form of pathology secondary to low ADH secretion by the posterior pituitary. Decreased levels of ADH will increase the number of binding receptors in the collecting duct. However, this will result in excess free water in the urine. Approximately 50% of the cases have no clear etiology and therefore, are labeled as idiopathic central diabetes insipidus. The other 50% belong to different categories including familial, traumatic and various disorders of hypothalamus, pituitary, for example, Sheehan syndrome, tumors, sarcoidosis, tuberculosis, syphilis, eosinophilic granuloma, and/or encephalitis.[3]

Oxytocin Insufficiency

Hyposecretion of oxytocin is not a common pathology but does transpire on rare occasions. This occurs when the level of oxytocin is well below the baseline margins. Low levels of oxytocin would halt uterine contraction and milk ejection during the birthing process. Panhypopituitarism is a condition where all anterior and posterior pituitary hormone levels are below normal, compromising their respective organs and can potentially be the cause of oxytocin insufficiency.[6]

Hypersecretion

Syndrome of Inappropriate Antidiuretic Hormone

SIADH is excess ADH production from the posterior pituitary or from an ectopic source. Elevated levels result in excess water retention and hypervolemic hyponatremia. However, edema or third spacing does not occur because hypervolemia activates natriuresis, which excretes water from the urine. SIADH also inhibits the production of renin and aldosterone secondary to volume expansion and high blood pressure, leading to diuresis. SIADH etiologies are tumors, malignancies, stroke, trauma, infection, medications, and/or anesthesia.[7]

Oxytocin Toxicity

This is a very rare situation and occurs when the level of oxytocin is above the baseline margins. High concentrations of oxytocin result in an overactive uterus causing hypertrophy and limiting pregnancy due to insufficient space to hold the fetus.

Central versus Peripheral Diabetes Insipidus

Patients with CDI and PDI present with excessive diluted urine (polyuria) and thirst (polydipsia). If the patient has an impaired thirst drive they may present with mild hypernatremia. Differentiating CDI and PDI requires checking urine osmolality and a water deprivation test. In Psychogenic polydipsia, the urine osmolarity will return to baseline within a couple hours after a water deprivation test. However, if the osmolarity continues to trend below baseline then injecting 2 g of desmopressin (vasopressin agonist) would be the next step. In central diabetes insipidus, the urine osmolarity should slowly return to baseline a couple of hours after administering desmopressin. In peripheral diabetes insipidus, there will be no change in urine osmolarity despite administration of desmopressin. The long-term treatment for CDI is desmopressin via nasal spray, orally, or subcutaneous injections. As for PDI, long-term treatment is with sodium depletion, thiazide diuretics, and removal of any offending agents, like lithium. Studies have shown that removing electrolytes from the body will lead to increase reabsorption of sodium and water in the proximal tubules.[3][4]

Syndrome of Inappropriate Antidiuretic Hormone  

SIADH is a diagnosis of exclusion once all other causes of hyponatremia have been ruled out. Treatment depends on the status of the patient, if asymptomatic then water restriction is sufficient. If this treatment fails to improve the sodium level, then add lithium or demeclocycline. Both agents antagonize antidiuretic hormone, which will induce diuresis and ultimately correct the sodium level. ADH receptor antagonists like tolvaptan and conivaptan are also approved for treatment of SIADH. In symptomatic patients, first-line treatment depends on the severity of the symptoms.[3][7]