Clinical manifestations of scrofula is often a part of miliary tuberculosis. Miliary TB is mostly subacute or chronic, less commonly, acute presentations. Subacute or chronic conditions may present with weight loss or failure to thrive. Fever, unusual rigors, frequent night sweats are the additional symptoms. These symptoms may begin 4 to 8 weeks earlier than the appearance of lymphadenopathy. History of progressively slow, painless mass starting in a single discrete group. Initially, 1 to 3 lymph nodes involvement is often unilateral (85%) is more common than bilateral. Symptoms are more in HIV infected than HIV-negative patients. The rate of symptoms is variable in different geographical areas in a series of studies, i.e., fever and weight loss more common in endemic areas than in the United States of America.[7]

On physical examinations, usually swelling on the one side of the neck, freely mobile, few in number, discrete, nontender lymph nodes are the common findings.[8] As time passes, swelling becomes firm, attached to surrounding tissues, rubbery and matted.[4] Ulceration, sinus draining (4% to 11%) caseous material with fluctuant swelling, fistula formation arise as time elapses.[7] Tenderness is positive in only 10% to 35% of cases. Size of the mass generally 3 cm but may be up to 8 to 10 cm. The involvement of lymph nodes in chain is 45% to 70%, mainly anterior groups affected.

In countries like Denmark, where incidence is low requires a high degree of suspicion to make the diagnosis.[6] Diagnosis in a high-risk population in endemic areas is not difficult who present with typical symptoms. HIV positive patients suffer from an immense number of neoplastic and infectious diseases; the diagnosis of cervical lymphadenopathy may be delayed. Early diagnosis of scrofula in these patients helps make early recognition of HIV allow early administration of antiretroviral therapy.[11]

Diagnosis of tuberculous lymphadenopathy is provided by histopathology along with a smear of acid-fast bacilli and culture of lymph nodes. Fine needle aspiration and excisional biopsy is the gold standard for diagnosis of peripheral lymphadenopathy, including tuberculous cervical lymphadenitis.[12]

Fine Needle Aspiration (FNA)

FNA is the diagnostic choice with low morbidity, relatively safe and inexpensive, rapid, and simple.[13] The sample specimen should be cultured, microscopic examination, cytologic interpretation, and send for polymerase chain reaction testing. Microscopy shows epithelioid granuloma or mycobacteria with the presence of or absence of giant cells and caseating necrosis.[9] In a series of evaluations performed in Hong Kong, specificity and sensitivity of FNA were found to be 93 and 77 percent, respectively.[13] The yield of FNA increases in HIV infected patients and endemic areas because of a high burden of microorganisms.

Mycobacterial Culture

Definitive diagnosis depends upon the isolation and identification of bacteria from specimens. Specimens inoculated in the egg or agar-based medium (Lowenstein-Jensen) incubated at 37-degree Celcius. Culture is positive in 70% to 80% of cases.

Excisional Biopsy

It is the traditional technique with higher morbidity than FNA and causes a delay in diagnosis.[9] It is a more invasive technique usually performed in the absence of less invasive techniques such as FNAC or ultrasound-guided FNAC. [14] Excisional biopsy has the highest yield that should be performed when FNAC is not diagnostic.[13] It is preferred over FNAC because FNAC has higher chances of iatrogenic fistula formation.

Nucleic Acid Amplification (NAA) Testing

NAA is performed where histology and acid-fast bacilli do not provide sufficient information for diagnosis.[15]

Imaging

Neck imaging is achieved by ultrasound, magnetic resonance imaging, and computed tomography scan. Imaging shows tracheal deviation if present, calcifications of lymph nodes, a cold abscess formed after coalesce of multiple necrotic lymph nodes.[9] CT scan is useful to monitor the treatment and take biopsy though it is not specific for TB. It helps to differentiate scrofula from other metastatic cancer or lymphoma. CT scan in disseminated tuberculosis shows peripheral and homogenous mixed with peripheral enhancement and multilocular appearance.[16] Chest imaging shows normal or widened mediastinum is performed in concomitant pulmonary tuberculosis.

Interferon-gamma Release Assay (IGRA)

IGRA has high sensitivity and specificity in the diagnosis of tuberculous lymphadenitis.[17] It is based upon the measurement of Interferon-gamma released from T-cells in response to stimulation with the highly TB-specific antigen.

Mantoux Test

Positive Mantoux test (skin injection of tuberculin with induration formation) shows a previous infection or latent TB.[13]

Treatment objectives are to prevent morbidity and mortality by killing the bacilli and interrupt the transmission.

Antitubercular Therapy

Antitubercular therapy is the treatment of choice against mycobacterial tuberculosis in both pulmonary and extrapulmonary tuberculosis. Treatment modality may be different for HIV positive patients because HIV infected patients have more bacteria resistant to isoniazid and streptomycin.[18] Generally, the standard regimen of therapy includes isoniazid (5 mg/kg), rifampicin (10 mg/kg), ethambutol 15 mg/kg), and pyrazinamide (25 mg/kg) all daily orally for 2 months as initial, or bactericidal phase followed by 4 months of continuation, or sterilizing phase in susceptible or in HIV negative patients with rifampin and isoniazid required to eliminate persisting mycobacteria that prevent relapse in adults. This regimen cure TB is greater than 90% of patients. In children without HIV infection or isoniazid resistance cane be treated without ethambutol. Three times weekly throughout the course can also be done in HIV negative patients, but HIV infected patients must be treated daily regimen of drugs throughout the course. If isoniazid resistance is more than 4%, the intensive (initial) phase should include streptomycin. Isoniazid only resistance can be treated for six months with additional streptomycin without isoniazid. Multidrug-resistance to both isoniazid and rifampicin should be treated with other drugs for 18 months of drugs. Drugs resistant have been increasing progressively, mainly due to lack of compliance to prescribed regimens, wrong prescription of regimens, and by primary resistance due to the spontaneous mutation of mycobacterial strains.

HIV-infected patients should have a longer duration of antitubercular therapy along with antiretroviral therapy addressing the drug interaction and adverse effects. Monitoring of treatment responses and drug toxicity should be done regularly to make complete and effective therapy.

Surgical Aspiration, Incision and Drainage, and Excision

Excision and removal of affected lymph nodes may be sufficient to treat scrofula in immunocompetent patients if no concomitant pulmonary TB and other associated extrapulmonary TB, especially in immunocompetent children infected by atypical mycobacteria.[19] But it does not work well in all cases as chances of high recurrence and fistula formation may spread the disease further. It is recommended in paradoxical upgrading reactions, treatment failure, discomfort due to tense, fluctuant lymph nodes, and children with cervical lymphadenitis by nontuberculous mycobacteria, which has better outcomes.[7]

Corticosteroid

The benefits of steroids in TB lymphadenitis are unknown, but double-blind controlled trials revealed improvements in those receiving a 37-day tapering dose of steroid. Steroids are used selectively in discomfort and not widely recommended.[7]

BCG Vaccination and Prevention

The best way to prevent TB is to diagnose and isolate cases rapidly and to administer appropriate therapy until patients become noninfectious and completely cured. Additional strategies include Bacille Calmette Guerin (BCG) vaccination. BCG is a live attenuated vaccine that was first administered in 1921. In addition, providing protection against tuberculosis, it also modulates immune responses to other vaccines.[20]

Differential diagnosis of scrofula is extensive, which includes lymphomas, metastatic cancer, and other infections that involve lymph nodes. It is challenging to extricate scrofula from lymphoma on clinical ground because both cases involve weight loss with other constitutional symptoms and from infectious lymphadenitis.

Lymphoma

Lymphoma is the neoplastic proliferation of lymphoid cells that forms a mass, may arise in lymph nodes or extranodal tissue. Hodgkin and non-Hodgkin lymphomas are the major types of lymphomas that may involve the cervical lymph nodes, which mimics the tuberculous lymphadenitis. Tuberculous lymphadenopathy and lymphoma can be differentiated by contrast-enhanced CT. Tuberculous lymphadenopathy shows peripheral enhancement with frequent multilocular, and non-treated lymphomas show homogenous attenuation.[16] Histopathology also helps to differentiate lymphoma (typical Reed Sternberg and lymphoid cells in Hodgkin and non-Hodgkin cells respectively) and Tuberculous cervical lymphadenopathy (granuloma with central caseous necrosis). Both lymphoma and scrofula are slow-growing, and painless may complicate the diagnosis.

Kikuchi Disease

It is an idiopathic self-limiting histiocytic necrotizing lymphadenitis that has strong mimicry with tuberculous cervical lymphadenitis. In the past, steroid therapy given to cervical lymphadenitis, assuming Kikuchi disease was not responding, and later on, surgical exploration confirmed TB. So only surgical exploration and microscopy helps in diagnosis.[21] Other associated clinical features of Kikuchi disease are fever, skin eruptions such as macules and papules. Microscopy of Kikuchi disease shows infiltration of CD163 and CD68 positive histiocytes predominantly and lymphocytes but no neutrophils, and nuclear debris.[22] Kikuchi is a benign disease that is a great mimicker with several diseases, including malignancy, so thorough workup is needed to exclude the other diseases.[23]

Fungal Diseases

Fungal diseases likely histoplasmosis, cryptococcus, Coccidioidomycosis, etc. has high chances to come up with tuberculosis as opportunistic diseases in HIV-infected people. Histopathology of fungal infected lymph nodes shows caseous necrosis with granuloma (chronic) formation.

Bacterial Adenitis

Bacterial lymphadenitis is caused by varieties of bacteria such as staphylococcus, streptococcus, catch-scratch disease (Bartonella), listeriosis, syphilis, etc. To date, cat-scratch disease caused by Bartonella henselae is most often encountered adenitis. Histological evaluation, culture, and polymerase chain reaction (PCR) have revolutionized the diagnosis.[24]

Kimura Disease

It is a rare idiopathic chronic inflammatory disease characterized by subcutaneous swelling in the head and neck region common in young Asian males usually associated with lymphadenopathy.[25]

Castleman Disease

Castleman disease is a generalized lymphoproliferative disorder of a poorly understood mechanism.[26]

Miscellaneous

Sarcoidosis (noncaseating granuloma), carotid body tumor, lymphosarcoma, toxoplasmosis, lymphosarcoma, neurofibroma, osteosarcoma, chondrosarcoma, salivary gland abscess, branchial cyst, aberrant thyroid glands, metastatic cancer, etc. in the neck upset the correct diagnosis of scrofula.[8]

Many differential diagnoses and low incidence of TB lymphadenitis delays the diagnosis, which increases morbidity and mortality.[6] The response of treatment in cervical lymphadenitis is slow, and lymph nodes may enlarge during or after treatment. HIV infected and debilitated patients have a poor prognosis and require long duration and daily therapy while immunocompetent have a better prognosis and even sometimes survive only be excision of involved lymph nodes. Diagnosis is complex, and disease is more severe in children under 5 yrs of age.[27] Complete history and proper investigations with diagnosis prompt the treatment in HIV patients gives a better response to both antitubercular and antiretroviral therapy. Clinical remission for Mycobacterium tuberculosis after medical treatment is up to 100% and for non-tuberculous mycobacterium is greater than 90% after surgical treatment. People who had TB once are at a higher risk of developing TB on reinfection.[28] People who have close contact, such as family members, are highly recommended for a tuberculosis test.

Less than half of the cases with scrofula also have pulmonary TB. There are chances that scrofula can spread beyond the neck and involve other areas of the body. Ulcerations, draining sinus, open wound, and finally, chronic fistula formation are the most probable sequelae of scrofula. Superimposed secondary bacterial infections in open wounds lead to further serious infection. Scar formation is more prominent with nontuberculous mycobacterial involvement. The relapse rate is up to 3.5% in patients treated for tuberculous lymphadenitis.[29]

The therapy-related paradoxical reaction occurs in about 25% during or after the cessation of treatment. Lymph nodes increase in size (79%), but it does not indicate treatment failure, worsening of the disease and development of new diseases in other organs and new lymph nodes (29%) in the same or different sites.[30] Trachea, carotid artery, esophagus compression due to mass effect by enormously large lymph nodes in rare cases can be spotted. Dissemination of Mycobacterium from primary cervical lymph node involvement leads to widespread miliary TB and multiorgan failure. Surgery related complications such as facial nerve involvement, fistula formation, and spread to surrounding tissue have limited the role of surgery. Complications in patients undergoing chemotherapy are hepatotoxicity, neurotoxicity, hemolysis, hyperuricemia, decreased visual acuity, etc.

Tuberculosis is a highly contagious infectious disease. The pattern of the disease to thrive on the basis of living standard and environmental sanitation makes it possible to predict the prevalence of disease so as to prevent it accordingly at the community level. Social awareness about the disease and individual effort at the patient level to isolate themselves has a significant role in controlling the disease. So patient education regarding the etiology and mode of transmission is always given the top priority for such communicable disease. People should be provided with information about the signs and symptoms of the disease so that they visit the clinician earlier stages before the dissemination of the disease-causing miliary TB and multiorgan failure. Correct information helps to delay the complications. The importance of scheduled therapy, compliance, and follow up should be notified. Detail information in regard to side effects and outcomes of therapy, development of drug resistance in negligence of regular intake should be delivered. Educating the patient about the extensive treatment methods depend upon the understanding level of the patients. 

The diagnosis of scrofula only on the clinical background is difficult.[31] Being infectious disease, most of the symptoms are non-specific that mimics the other infectious disease, which hampers the diagnosis, and also, the signs and physical findings are challenging due to resembling other diverse neck swellings. Therefore the interprofessional healthcare team is highly appreciated and must for making prompt and right diagnoses. Infectious disease specialist helps to exclude other infectious diseases, oncologists for lymphoma exclusion, surgeons for surgical excision of the cervical mass, epidemiologist to study the pattern of the disease process, Histopathologists for biopsy, pharmacists to monitor the accurate drugs with their dose, duration and mode administration, nursing care of patients, radiologists for radiodiagnosis, microbiologists for culture and identification of causative agent, etc. Knowledge of other diseases such as Kimura disease will place the physician in a better position to rule out and diagnose the disease. Government authority, such as the Center for Disease Control and Prevention, plays a huge role in collecting data and monitoring the epidemiology of the disease. Social and preventive medicine has a vital role in prevention at the root level.