As the largest of the cranial nerves, the trigeminal nerve is responsible for the primary sensory input from the head and neck as well as providing motor innervation to the muscles of mastication. The trigeminal nerve innervates key vascular structures such as the brainstem, the cavernous sinus, and peripheral divisions. With such an extensive distribution within the head and neck, various lesions may contribute to trigeminal neuralgia or dysfunction. The goal of this activity is to review the neuroanatomy of the trigeminal nerve and cover the reflex tests employed to distinguish between lesions of the trigeminal nerve.

The trigeminal nerve is the fifth cranial nerve (CN V) and the largest of the paired cranial nerves. It is a mixed nerve that partially innervates the craniofacial region along with the facial nerve. The fifth cranial nerve contains three terminal branches that innervate the skin of the face and neck, mucous membranes and paranasal sinuses of the face, the corneas, and the muscles of mastication. A trigeminal nerve reflex is a natural response to stimuli indicating the proper functioning of the nerve. Each area innervated by the trigeminal nerve can undergo assessment through the presence and strength of a trigeminal nerve reflex.[1][2][3][4]

Structure and Function:

The complexity of its neuroanatomy matches the size of the CN V. The trigeminal nerve fibers arise from the trigeminal nerve nucleus, which extends from the midbrain to the upper cervical spinal cord. The trigeminal nerve nucleus is the largest of all cranial nerve nuclei. It divides into three main portions: (1) the mesencephalic trigeminal nucleus, which receives mechanoreceptor fibers and proprioceptive information from the mandibles and teeth, (2) the main trigeminal nucleus, which receives the majority of the touch and position fibers, and the (3) spinal trigeminal nucleus which primarily receives temperature and pain stimuli.[2][1][5][6]

The sensory nuclei merge at the level of the pons to form a sensory root. The motor nuclei continue inferiorly to the sensory nuclei to form a motor root. The sensory root then expands into the trigeminal ganglion at the level of the middle cranial fossa or Meckel’s cave. It is within this fossa that the nerves combine to form the semilunar ganglion. The position of the superior petrosal sinus is located medially to these structures. The basilar venous plexus and ventral aspects of the pons and brainstem are medial to the Meckel’s cave.[2][7][8]

Upon exiting the trigeminal ganglion, the fibers of CNV take a superomedial route toward the pons. At this point, both divisions of CN V pierce the lateral surface of the pons. Within the pons, the sensory fibers divide equally into ascending and descending divisions. Ascending fibers take a superior course towards the mesencephalic nucleus while the descending fibers will take a route toward the spinal trigeminal nucleus. Remaining sensory fibers follow a dorsomedial course toward the main sensory nucleus. Simultaneous to the dorsomedial travel of the sensory fibers toward the sensory nucleus, the remaining motor fibers will follow a similar path toward their respective motor nuclei.[2][5][9]

The bilateral pair of the mesencephalic nucleus consists of a collection of unipolar neurons extending from the main sensory nucleus within the pons. It is primarily involved in processing information related to proprioception. Myelinated axons exit the mesencephalic tract and divide into central and peripheral divisions. Central branches conduct impulses from the neuromuscular spindles of the bite-force reflex arcs within the muscles of mastication toward the motor neuron of CN V. Several other central branch axons integrate with the sensory aspect of CN V via the reticular formation or migrate toward the cerebellum via the superior cerebellar peduncle. The extensive networking of the trigeminal nerve motor and sensory divisions contribute to the regulation of the masticatory stretch muscles and the process of deglutition. The mesencephalic nucleus peripheral branches travel as fibers via the maxillary branch of the CN V in the upper jaw and the mandibular branch of the CN V in the lower jaw.[1][2][7]

Lateral to the motor nucleus of CN V lies the main sensory nucleus inside of the dorsal portion of the pontine tegmentum. Fibers derived from the mesencephalic nucleus and afferent spinal axons feed proprioceptive impulses toward the main sensory nucleus. Various larger fibers also convey discriminative touch and light touch impulses toward the main sensory nucleus. Neurons within the main sensory nucleus from the trigeminal nuclei and the pontine coalesce as the ventral trigeminothalamic tract. Other fibers within the main nucleus travel toward the thalamus forming the dorsal trigeminothalamic tract. The ventral and dorsal tracts coalesce in the area of the pons adjacent to the midbrain and are collectively known as the trigeminal lemniscus tract. Fibers within this tract access the ventral posterior medial aspect of the thalamus and ascend into an internal capsule toward the postcentral gyrus in which sensory information gets processed.[2][10][7]

The CN V motor nucleus is a conglomerate of multipolar neurons medially situated to the pontine trigeminal nucleus. The tracts of the motor nucleus innervate certain muscles within the first pharyngeal arch, including the pterygoid muscles, the masseter, and temporalis muscles. The motor nucleus is deep to the lateral rhomboid fossa in the upper division of the pontine tegmentum. The motor axons eventually merge with the mandibular V3 division of CN V. The motor nucleus is regulated through sensory input from the cerebral cortices bilaterally, as well as via the afferent fibers of the mesencephalic nuclei.[10][11][12]

Spanning the full length of the medulla oblongata to the proximal spinal cord at the level of the second or third cervical segment is the spinal nucleus of CN V. The spinal trigeminal nucleus divides into three smaller nuclei based on location. Most proximal to caudal, these are the pars oralis, pars interpolaris, and pars caudalis. The spinal trigeminal tract feeds afferent fibers, while non-myelinated central processes from the ganglion feed into the spinal nucleus. These fibers descend by way of the superior pontine sulcus after passing through the pons and carry sensory information from the three CN V divisions. The fibers of the trigeminal tract have a specific somatotopic organization in which fibers of the CN V2 run centrally and are flanked ventrolaterally and dorsomedially by the ophthalmic and mandibular divisions respectively. These tracts also contain general somatic afferent fibers arising from other cranial nerves like the vagus (CNX), glossopharyngeal (CN IX), and facial (CN VII) nerves.[13][1][10][5]

Trigeminal Nerve Divisions:

The sensory aspect of CN V provides the primary innervation to the brainstem, skin of the face, paranasal sinuses, cornea, facial and masticatory muscles, the temporomandibular joint (TMJ), most of the dura mater and cerebral arteries. The brachial motor nerves of CN V innervate the masticatory muscles (including the masseter, lateral and medial pterygoids, and the temporalis), the mylohyoid, tensor veli palatini, and the tensor tympani. The CN V further divides into three main branches: the ophthalmic branch (V1), the maxillary branch (V2), and the mandibular branch (V3). Although there are no innate parasympathetic nerve fibers within CN V, the main branches are associated with several parasympathetic ganglia throughout the course of innervation.[1][11][5][7]

The ophthalmic branch of CN V additionally divides into three terminal branches: the frontal, lacrimal, and nasociliary. These terminal branches innervate the frontonasal prominence derivatives and mucous membranes of the cornea, dorsum of the nose, forehead, and scalp, the frontal and ethmoid sinus, and the upper eyelid along with its conjunctiva. The lacrimal gland receives innervation from the lacrimal branch of CN V as well as parasympathetic innervation from the postganglionic fibers from the pterygopalatine ganglion derived from CN VII.[3][14][15]

 The maxillary branch of CN V is additionally divided into fourteen terminal branches innervating the mucous membranes and sinuses of derivatives of the maxillary prominence of the first pharyngeal arch. These structures include the superior palate, upper lip, upper molar, and associated gingiva, nasal cavity and lateral nose, lower eyelid and its conjunctiva, and the maxillary sinus. Similar to the ophthalmic branch, the postganglionic fibers from the pterygopalatine ganglion of CN VII join with V2 to supply parasympathetic innervation to the lacrimal gland. The nasal glands also receive parasympathetic innervation from fibers that travel alongside the nasopalatine and greater palatine nerve branches of V2.[1][2][11][16]

The mandibular branch of CN V is further subdivides into four terminal branches within the infra-temporal fossa: alveolar, auriculotemporal, buccal, and lingual nerves. These nerves supply sensory innervation to the external ear, chin, lower lip, anterior two-thirds of the tongue, lower molars, and associated gingiva, and the mucous membranes of the floor of the oral cavity. The mandibular branch is the only branch of the CN V with motor innervation, as well. The supplied structures include the muscles of mastication (medial and lateral pterygoids, masseter, temporalis), tensor veli palatini, tensor tympani, and the suprahyoid muscles. The submandibular and sublingual glands receive parasympathetic innervation via the post-ganglionic nerve fibers from CN VII that travel along with the lingual nerve. The parotid gland also receives parasympathetic innervation via fibers of CN IX that travel parallel to the auriculotemporal branch of V3.[13][11]