Arthrobacter luteus

Arthrobacter luteus
Scientific classification
Domain: Bacteria
Phylum: Actinomycetota
Order: Actinomycetales
Family: Micrococcaceae
Genus: Arthrobacter
Species:
A. luteus
Binomial name
Arthrobacter luteus
Kaneko et al., 1969

Arthrobacter luteus (ALU) is a species of gram-positive bacteria in the genus Arthrobacter. A. luteus is facultatively anaerobic, pleomorphic, branching, non-motile, non-sporulating, non-acid-fast, catalase-positive and rod-shaped (0.6-1.0 x 0.8-10.0 μm x μm).[1] All members of the Arthrobacter genus are obligate aerobes.

A restriction endonuclease enzyme is extracted from the bacterium and acts at the centre of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'-end. The restriction site Alu-I itself is a 4-base cutter: AG/CT.[2] The Alu retrotransposon is named after the bacterium's abbreviation. The bacterium is also used to produce zymolyase,[3] which can degrade yeast cell wall.

A endonuclease restriction that occurs from Arthrobacter luteus has been isolated and the nucleotide sequence at the cleave site of the restriction isolated. The cleaveage occurs at the center of the palindromic tetranucleotide sequence, which gives even-ended duplex DNA fragments phosphorylated at the 5’-end.[4]

References

  1. ↑ Kaneko, Tatsuhiko (1969). "Arthrobacter Luteus Nov. Sp. Isolated From Brewery Sewage". Journal of General and Applied Microbiology. 15 (3): 317–326. doi:10.2323/jgam.15.317.
  2. ↑ Abdurashitov, Murat A.; Tomilov, Victor N.; Chernukhin, Valery A.; Degtyarev, Sergey (2008). "A physical map of human Alu repeats cleavage by restriction endonucleases". BMC Genomics. 9: 305. doi:10.1186/1471-2164-9-305. PMC 2443384. PMID 18578890.
  3. ↑ Kitamura, Kumpei; Kaneko, Tatsuhiko; Yamamoto, Yasushi (1974). "LYSIS OF VIABLE YEAST CELLS BY ENZYMES OF ARTHROBACTER LUTEUS". The Journal of General and Applied Microbiology. 20 (6): 323–344. doi:10.2323/jgam.20.323.
  4. ↑ Jay, Ernest; Wu, Ray (1976-08-01). "Nucleotide sequence analysis of DNA. 28. Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA". Biochemistry. 15 (16): 3612–3620. doi:10.1021/bi00661a032. ISSN 0006-2960.


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