Mycolicibacter

Mycolicibacter
Scientific classification
Domain:
Bacteria
Phylum:
Actinomycetota
Class:
Actinomycetia
Order:
Mycobacteriales
Family:
Mycobacteriaceae
Genus:
Mycolicibacter Gupta et al. 2018

Mycolicibacter is a genus of Gram-Positive rod-shaped bacteria in the family Mycobacteriaceae from the order Mycobacteriales.[1] The type species of this genus is Mycolicibacter terrae.

Members of Mycolicibacter were demarcated from the larger genus Mycobacterium in 2018 by Gupta et al. based on evidence from various phylogenetic trees constructed based on conserved genome sequences, comparative genomic analyses and average amino acid identity values.[1] In addition to this genus, the study proposed the division of Mycobacterium into a total of five distinct genera, which was met with some resistance by some of the scientific community. The resistance was based on the grounds that Mycobacterium contains some clinically relevant species and name changes might cause confusion among clinicians and other researchers.[2]

In 2020, Yamada et al. analyzed the fundamental morphological properties of the new genera, including the cell diameter, cell length, cell perimeter, cell circularity and aspect ratio, and determined that there were significant differences between the five genera, thus supporting the new division.[3]

The name Mycolicibacter is derived from the Latin noun acidum mycolicum (translates to mycolic acid) and the Latin noun bacter (translates to rod). Together the name refers to a genus of mycolic acid containing rod-shaped bacteria.[1]

Biochemical Characteristics and Molecular Signatures[1]

Members of Mycolicibacter are slow-growing bacterial species (taking more than seven days to form colonies) and nonchromogenic (does not produce pigment). Some species grow at an intermediate rate, taking 5-15 days to form colonies. While most members of Mycolicibacter are non-pathogenic, some species have been isolated from animal hosts and human patients. They have a higher G+C content (ranging between 66.3-70.3 mol%) and relatively short genomes between the range of 3.87-5.11 Mbp.

Analyses of genome sequences from Mycolicibacter identified 26 conserved signature indels (CSIs) that are specific for this genus in the proteins such as non-ribosomal peptide synthetase, nucleoside hydrolase, TetR family transcriptional regulator, carbon starvation protein A, error-prone DNA polymerase, amidohydrolase, carboxymunconolacton decarboxylase family protein, polyketide cyclase, spirocyclase AveC family protein, TobH protein, UDP-N-acetylmuramate–L-alanine ligase, DUF2236 domain-containing protein, cobaltochelatase subunit CobN, alpha/beta hydrolase, potassium transporter Kef, a membrane protein, DUF222 domain-containing protein, MFS transporter, adenylate/guanylate cyclase domain-containing protein, DUF2029 domain-containing protein and hypothetical proteins.[1] These CSIs provide a reliable molecular method for identifying and distinguishing members of Mycolicibacter from other genera in the family Mycobacteriaceae. Additionally, 15 unique conserved signature proteins (CSPs) were identified to be specific to most or all members of this genus.

References

  1. 1 2 3 4 5 Gupta, Radhey S.; Lo, Brian; Son, Jeen (2018-02-13). "Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera". Frontiers in Microbiology. 9: 67. doi:10.3389/fmicb.2018.00067. ISSN 1664-302X. PMC 5819568. PMID 29497402.
  2. ↑ Tortoli, Enrico; Brown-Elliott, Barbara A.; Chalmers, James D.; Cirillo, Daniela M.; Daley, Charles L.; Emler, Stefan; Floto, R. Andres; Garcia, Maria J.; Hoefsloot, Wouter; Koh, Won-Jung; Lange, Christoph (2019-07-01). "Same meat, different gravy: ignore the new names of mycobacteria". European Respiratory Journal. 54 (1). doi:10.1183/13993003.00795-2019. ISSN 0903-1936. PMID 31296783. S2CID 195893005.
  3. ↑ Yamada, Hiroyuki; Chikamatsu, Kinuyo; Aono, Akio; Murata, Kazuyoshi; Miyazaki, Naoyuki; Kayama, Yoko; Bhatt, Apoorva; Fujiwara, Nagatoshi; Maeda, Shinji; Mitarai, Satoshi (2020). "Fundamental Cell Morphologies Examined With Cryo-TEM of the Species in the Novel Five Genera Robustly Correlate With New Classification in Family Mycobacteriaceae". Frontiers in Microbiology. 11: 562395. doi:10.3389/fmicb.2020.562395. ISSN 1664-302X. PMC 7701246. PMID 33304323.
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