Outer membrane vesicles

Transmission electron micrograph of outer membrane vesicles (OMV) (size 80-90 nm, dia) released by human pathogen Salmonella 3,10:r:- in chicken ileum, in vivo. OMVs were proposed to be 'blown off' from large bacterial periplasmic protrusions, called periplasmic organelles (PO) with the help of 'bubble tube'-like assembly of about four type III secretion injectisomal rivet complexes (riveting bacterial outer and cell membrane to allow pockets of periplasm to expand into POs). This allows membrane vesicle trafficking of OMVs from gram negative bacteria to dock on host epithelial cell membrane (microvilli), proposed to translocate signal molecules from pathogen to host cells at the host-pathogen interface.

Outer membrane vesicles (OMVs) are vesicles released from the outer membranes of Gram-negative bacteria. While Gram-positive bacteria release vesicles as well those vesicles fall under the broader category of bacterial membrane vesicles (MVs). OMVs were the first MVs to be discovered, and are distinguished from outer inner membrane vesicles (OIMVS), which are gram-negative baterial vesicles containing portions of both the outer and inner bacterial membrane.[1] Outer membrane vesicles were first discovered and characterized using transmission-electron microscopy[2] by Indian Scientist Prof. Smriti Narayan Chatterjee and J. Das in 1966-67.[3][4] OMVs are ascribed the functionality to provide a manner to communicate among themselves, with other microorganisms in their environment and with the host. These vesicles are involved in trafficking bacterial cell signaling biochemicals, which may include DNA, RNA, proteins, endotoxins and allied virulence molecules. This communication happens in microbial cultures in oceans,[5] inside animals, plants and even inside the human body.[6]

Gram-negative bacteria deploy their periplasm to secrete OMVs for trafficking bacterial biochemicals to target cells in their environment. OMVs also carry endotoxic lipopolysaccharide initiating disease process in their host.[7] This mechanism imparts a variety of benefits like, long-distance delivery of bacterial secretory cargo with minimized hydrolytic degradation and extra-cellular dilution, also supplemented with other supportive molecules (e.g., virulence factors) to accomplish a specific job and yet, keeping a safe-distance from the defense arsenal of the targeted cells. Biochemical signals trafficked by OMVs may vary largely during 'war and peace' situations. In 'complacent' bacterial colonies, OMVs may be used to carry DNA to 'related' microbes for genetic transformations, and also translocate cell signaling molecules for quorum sensing and biofilm formation. During 'challenge' from other cell types around, OMVs may be preferred to carry degradation and subversion enzymes. Likewise, OMVs may contain more of invasion proteins at the host-pathogen interface (Fig. 1). It is expected, that environmental factors around the secretory microbes are responsible for inducing these bacteria to synthesize and secrete specifically-enriched OMVs, physiologically suiting the immediate task. Thus, bacterial OMVs, being strong immunomodulators,[8] can be manipulated for their immunogenic contents and utilized as potent pathogen-free vaccines[9] for immunizing humans and animals against threatening infections. VA-MENGOC-BC and Bexsero against meningitis are currently the only OMV vaccines approved in the US, though an OMV vaccine for gonorrhea is seeking approval.[10][11]

Biogenesis and movement

Gram-negative bacteria have a double set of bilayers. An inner bilayer, the inner cell membrane, encloses the cytoplasm or cytosol. Surrounding this inner cell membrane there is a second bilayer called the bacterial outer membrane. The compartment or space between these two membranes is called the periplasm or periplasmic space. In addition, there is a firm cell wall consisting of peptidoglycan layer, which surrounds the cell membrane and occupies the periplasmic space. The peptidoglycan layer provides some rigidity for maintaining the bacterial cell shape, besides also protecting the microbe against challenging environments.

The first step in biogenesis of gram-negative bacterial OMVs,[12] is bulging of outer membrane above the peptidoglycan layer. Accumulation of phospholipids in the outside of the outer membrane is thought to be the basis of this outwards bulging of the outer membrane.[13] This accumulation of phospholipids can be regulated by the VacJ/Yrb ABC transport system that transfers phospholipids from the outside of OM to the inner side.[13] Additionally, environmental conditions as sulfur depletion can trigger a state of phospholipid overproduction that causes increased OMV release.[14]

The actual release of the vesicle from the outer membrane remains unclear. It is likely that vesicle structures can be released spontaneously. Alternatively, it has been suggested that few proteins 'rivet' the outer and cell membranes together, so that the periplasmic bulge protrudes like a 'ballooned' pocket of inflated periplasm out from the surface of the outer membrane. Lateral diffusion of 'rivet complexes' may help in pinching off large bulges of periplasm as OMVs.[15]

Bacterial membrane vesicles dispersion along the cell surface was measured in live Escherichia coli, commensal bacteria common in the human gut. Antibiotic treatment altered vesicle dynamics, vesicle-to-membrane affinity, and surface properties of the cell membranes, generally enhancing vesicle transport along the surfaces of bacterial membranes and suggesting that their motion properties could be a signature of antibiotic stress.[16] Despite this first high-resolution, quantitative tracking of bacterial OMVs, detailed experimental work is still awaited to understand the biomechanics of OMV biogenesis and transport. OMVs are also under focus of current research in exocytosis in prokaryotes via outer membrane vesicle trafficking for intra-species, inter-species and inter-kingdom cell signaling, which is slated to change our mindset on virulence of microbes, host-pathogen interactions and inter-relationships among variety of species in earth's ecosystem.

See also

References

  1. ↑ Toyofuku, Masanori; Nomura, Nobuhiko; Eberl, Leo (January 2019). "Types and origins of bacterial membrane vesicles". Nature Reviews Microbiology. 17 (1): 13–24. doi:10.1038/s41579-018-0112-2. ISSN 1740-1534. PMID 30397270. S2CID 53224716.
  2. ↑ Chatterjee, S. N.; Das, J. (1967). "Electron microscopic observations on the excretion of cell-wall material by Vibrio cholerae". Journal of General Microbiology. 49 (1): 1–11. doi:10.1099/00221287-49-1-1. ISSN 0022-1287. PMID 4168882.
  3. ↑ "INSA :: Indian Fellow Detail". www.insaindia.res.in. Retrieved 2019-12-13.
  4. ↑ Anand, Deepak; Chaudhuri, Arunima (2016-11-16). "Bacterial outer membrane vesicles: New insights and applications". Molecular Membrane Biology. 33 (6–8): 125–137. doi:10.1080/09687688.2017.1400602. ISSN 0968-7688. PMID 29189113.
  5. ↑ Biller JJ, Schubotz F, Thompson AW, Summons RE and Chisholm SW (2014) Bacterial vesicles in marine ecosystems. Science, vol. 343(no. 6167), pp. 183-186.https://www.science.org/doi/abs/10.1126/science.1243457
  6. ↑ Tulkens, Joeri; Vergauwen, Glenn; Van Deun, Jan; Geeurickx, Edward; Dhondt, Bert; Lippens, Lien; De Scheerder, Marie-Angélique; Miinalainen, Ilkka; Rappu, Pekka; De Geest, Bruno G; Vandecasteele, Katrien; Laukens, Debby; Vandekerckhove, Linos; Denys, Hannelore; Vandesompele, Jo; De Wever, Olivier; Hendrix, An (5 December 2018). "Increased levels of systemic LPS-positive bacterial extracellular vesicles in patients with intestinal barrier dysfunction". Gut. 69 (1): gutjnl–2018–317726. doi:10.1136/gutjnl-2018-317726. PMC 6943244. PMID 30518529.
  7. ↑ YashRoy R C (1993) Electron microscope studies of surface pili and vesicles of Salmonella 3,10:r:- organisms. Indian Journal of Animal Sciences, vol. 63 (No.2), pp. 99-102. https://www.academia.edu/7327498/YashRoy_R_C_1993_Electron_microscope_studies_of_suraface_pili_and_vesicles_of_Salmonella_3_10_r_-_organisms.i_and_vesicles._Indian_Journal_of_Animal_Sciences._Vol_63_No.2_pp._99-102
  8. ↑ Ellis TN and Kuehn MJ (2010) Virulence and immuno-modulatory roles of bacterial outer membrane vesicles. Microbiology and Molecular Biology Reviews, vol. 74 (no. 1), pp. 81-94.http://mmbr.asm.org/content/74/1/81.short
  9. ↑ Acevedo, R; Fernandez, S; Zayas, C; Acosta, D; Sarmiento, ME; Ferro, VA; Rosenquvist, E; Campa, C; Cardoso, D; Garcia, L; Perez, JL (2014). "Bacterial outer membrane vesicles and vaccine applications". Frontiers in Immunology. 5: 121. doi:10.3389/fimmu.2014.00121. PMC 3970029. PMID 24715891.
  10. ↑ Lieberman, Linda (21 December 2022). "Outer membrane vesicles: A bacterial-derived vaccination system". Frontiers in Microbiology. 13. doi:10.3389/fmicb.2022.1029146. PMC 9811673. PMID 36620013.
  11. ↑ "GSK's gonorrhoea vaccine receives FDA's 'fast-track' designation". Reuters. 27 June 2023. Retrieved 20 August 2023.
  12. ↑ Kulp, A; Kuehn, MJ (2010). "Biological functions and biogenesis of secreted bacterial outer membrane vesicles". Annual Review of Microbiology. 64: 163–184. doi:10.1146/annurev.micro.091208.073413. PMC 3525469. PMID 20825345.
  13. 1 2 Roier, Sandro; Zingl, Franz G.; Cakar, Fatih; Durakovic, Sanel; Kohl, Paul; Eichmann, Thomas O.; Klug, Lisa; Gadermaier, Bernhard; Weinzerl, Katharina; Prassl, Ruth; Lass, Achim (2016-01-25). "A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria". Nature Communications. 7 (1): 10515. Bibcode:2016NatCo...710515R. doi:10.1038/ncomms10515. ISSN 2041-1723. PMC 4737802. PMID 26806181.
  14. ↑ Gerritzen, Matthias J. H.; Martens, Dirk E.; Uittenbogaard, Joost P.; Wijffels, René H.; Stork, Michiel (2019-03-18). "Sulfate depletion triggers overproduction of phospholipids and the release of outer membrane vesicles by Neisseria meningitidis". Scientific Reports. 9 (1): 4716. Bibcode:2019NatSR...9.4716G. doi:10.1038/s41598-019-41233-x. ISSN 2045-2322. PMC 6423031. PMID 30886228.
  15. ↑ YashRoy R C (2003) Eucaryotic cell intoxication by Gram-negative organisms: A novel bacterial outermembrane-bound nanovesicular model for Type-III secretion system. Toxicology International, vol. 10 (No. 1), 1-9.https://www.academia.edu/7695646/YashRoy_R_C_2003_Eukaryotic_cell_intoxication_by_Gram-negative_pathogens_A_novel_bacterial_outer_membrane-bound_nanovesicular_exocytosis_model_for_Type-III_secretion_system._Toxicology_International._Vol._10_No._1_pp._1-9
  16. ↑ Bos J, Cisneros LH, Mazel D (January 2021). "Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure". Science Advances. 7 (4): eabd1033. doi:10.1126/sciadv.abd1033. PMC 7817102. PMID 33523924.
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