Plesiocystis pacifica

Plesiocystis pacifica
Scientific classification
Domain:
Bacteria
Phylum:
Myxococcota
Class:
Polyangia
Order:
Nannocystales
Family:
Nannocystaceae
Genus:
Plesiocystis
Species:
P. pacifica
Binomial name
Plesiocystis pacifica
Iikuza 2003[1][2]

Plesiocystis pacifica is a species of marine myxobacteria. Like other members of this order, P. pacifica is a rod-shaped Gram-negative bacterium that can move by gliding and can form aggregates of cells called fruiting bodies. The species was first described in 2003, based on two strains isolated from samples collected from the Pacific coast of Japan.[1]

Characteristics and morphology

P. pacifica cells are straight, rod-shaped, and have blunt ends. Like other myxobacteria, groups of cells can form round fruiting bodies. The species is an obligate aerobe and a chemoorganotroph. Cells are motile and move by gliding. The species is mesophilic and grew best under laboratory conditions in a temperature range of 15-32C, a neutral to slightly basic pH, and salinity similar to that of seawater (making it slightly halophilic). P. pacifica cells require sodium to grow, as expected for marine bacteria.[1]

Taxonomy

P. pacifica is the type species and only member of the genus Plesiocystis, both first described in the same 2003 study. Based on comparisons of the P. pacifica 16S rRNA genetic sequence to those of other similar organisms, it is most closely related to bacteria of the genus Nannocystis, specifically Nannocystis exedens. The genus name Plesiocystis derives from the Greek words plesion (neighbor) and cystis (bladder), noting the genus' relationship to Nannocystis. The specific epithet refers to the species' discovery on the Pacific coastline of Japan.[1]

Metabolism

P. pacifica is a chemoorganotrophic predatory bacterium and can derive energy from decomposing living Gram-negative bacteria such as Escherichia coli.[1][3] It is an aerobe that uses oxygen as its terminal electron acceptor in its electron transport chain. It is unusual in using partially saturated menaquinones in this pathway, a characteristic otherwise more common in Gram-positive bacteria. P. pacifica is also unusual in the types of polyunsaturated fatty acids it produces, among which are long-chain forms not otherwise common in myxobacteria (although the quantities of these vary based on cultivation conditions).[1][4][5] The significance of this observation is unclear, as these compounds are found mainly in psychrophilic bacteria.[1]

Marine myxobacteria are relatively uncommon compared to terrestrial myxobacteria, such as P. pacifica's relative N. exedens. Studies of the salt adaptation of marine myxobacteria have found that P. pacifica accumulates exogenous amino acids from its environment as osmoprotectants, in contrast with the marine myxobacterium Enhygromyxa salina, which express genes for the synthesis of endogenously produced osmoprotectant molecules.[3]

Genome

Myxobacterial genomes typically have high GC content; in P. pacifica the GC content is 69-70%.[1]

Comparative genomics studies have identified myxobacteria and specifically P. pacifica as possessing an unusual kinome, with a high number of distinct "eukaryotic-like" serine/threonine and tyrosine kinase genes. These kinases are common regulatory proteins in eukaryotes, but are relatively rare in prokaryotes. The functions of these kinases in myxobacteria are unclear.[6] Similar bioinformatic analysis has identified a large number of genes in the P. pacifica genome associated with sterol production, which is quite rare among prokaryotes but ubiquitous in eukaryotes. There is evidence that at least some of the sterol-associated genes in P. pacifica derive from horizontal gene transfer from eukaryotes.[7]

References

  1. 1 2 3 4 5 6 7 8 Iizuka, T. (1 January 2003). "Plesiocystis pacifica gen. nov., sp. nov., a marine myxobacterium that contains dihydrogenated menaquinone, isolated from the Pacific coasts of Japan". International Journal of Systematic and Evolutionary Microbiology. 53 (1): 189–195. doi:10.1099/ijs.0.02418-0. PMID 12656172.
  2. ↑ "Plesiocystis". List of Prokaryotic Names with Standing in Nomenclature.
  3. 1 2 Schäberle, Till F.; Galinski, Erwin A.; Boehringer, Nils; Moghaddam, Jamshid Amiri; Kunte, Hans-Jörg; Burdziak, Amal (1 April 2016). "Different strategies of osmoadaptation in the closely related marine myxobacteria Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1". Microbiology. 162 (4): 651–661. doi:10.1099/mic.0.000250. PMID 26842314.
  4. ↑ Garcia, R.; Pistorius, D.; Stadler, M.; Muller, R. (11 February 2011). "Fatty Acid-Related Phylogeny of Myxobacteria as an Approach to Discover Polyunsaturated Omega-3/6 Fatty Acids". Journal of Bacteriology. 193 (8): 1930–1942. doi:10.1128/JB.01091-10. PMC 3133044. PMID 21317327.
  5. ↑ Garcia, Ronald; Müller, Rolf (2014). "The Family Nannocystaceae". The Prokaryotes: Deltaproteobacteria and Epsilonproteobacteria. pp. 213–229.
  6. ↑ Perez, J.; Castaneda-Garcia, A.; Jenke-Kodama, H.; Muller, R.; Munoz-Dorado, J. (3 October 2008). "Eukaryotic-like protein kinases in the prokaryotes and the myxobacterial kinome". Proceedings of the National Academy of Sciences. 105 (41): 15950–15955. Bibcode:2008PNAS..10515950P. doi:10.1073/pnas.0806851105. PMC 2572974. PMID 18836084.
  7. ↑ Desmond, E.; Gribaldo, S. (10 September 2009). "Phylogenomics of Sterol Synthesis: Insights into the Origin, Evolution, and Diversity of a Key Eukaryotic Feature". Genome Biology and Evolution. 1: 364–381. doi:10.1093/gbe/evp036. PMC 2817430. PMID 20333205.
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