Synechococcus elongatus

Synechococcus elongatus
Scientific classification
Domain: Bacteria
Phylum: Cyanobacteria
Class: Cyanophyceae
Order: Synechococcales
Family: Synechococcaceae
Genus: Synechococcus
Species:
S. elongatus
Binomial name
Synechococcus elongatus
(Nägeli) Nägeli

Synechococcus elongatus is a unicellular cyanobacterium that has a rapid autotrophic growth comparable to yeast. Its ability to grow rapidly using sunlight has implications for biotechnological applications, especially when incorporating genetic modification.

Occurrence

In the last decade, several strains of Synechococcus elongatus have been produced in laboratory environments, which ultimately led to the production of Synechococcus elongatus UTEX 2973. S. elongatus UTEX 2973 is a mutant hybrid from UTEX 625.[1]

In 1955, William A. Kratz and Jack Myers described a fast-growing cyanobacterial strain, Anacystis nidulans which was deposited in the University of Texas algae culture collection as Synechococcus leopoliensis UTEX 625[1][2] However, that strain had lost its rapid growth property and was also unable to grow at high temperatures, unlike the original strain. In 2015, Jingjie Yu and colleagues, were able to isolate the mutant strain from a mixed culture of Synechococcus UTEX 625. The mutant strain was deposited to the UTEX algae culture collection, and given a new number, UTEX 2979.[1]

Structure

Synechococcus elongatus is rod-shaped with its cells typically greater than 2 µm in length. It typically contains 2–3 thylakoid membrane layers forming evenly spaced concentric rings and its carboxysomes and polyphosphate bodies are located in the central cytoplasmic region (Image 1).[1]

Genetics

The genome sequence of Synechococcus UTEX 2973 was similar to the cyanobacterium Synechococcus PCC 7942. Even though it was isolated from S. elongatus 625, it is most closely related to S. elongatus PCC 7942 with 99.8% similarity. S. elongatus UTEX 2973 contains a SNP to the gene encoding ATP synthase F1 subunit α, comparable to the corresponding gene in Synechococcus PCC 7942. This specific SNP causes an amino acid substitution at the 252nd position of the protein.[1]

Metabolism

Synechococcus elongatus UTEX 2973 is photoautotrophic and has one of the shortest doubling times reported for cyanobacteria at 1.5 hours in a BG11 medium at 42 °C under continuous 1,500 μmoles photons·m−2·s−1 white light with 5% CO2.[3] While it is typically maintained on BG11 media, it can also be cryopreserved using 1% (v/v) dimethyl sulfoxide (DMSO) as a cryoprotectant.[4]


Significance

Ungerer and colleagues, 2018, used CRISPR/Cpf1, to perform a mutational analysis of S. elongatus UTEX 2973 by identifying the three genes with SNPs associated with rapid growth, atpA, ppnK, and rpaA. They replaced these fast-growth alleles with the wild-type alleles in the broadly studied cyanobacterium, Synechococcus 7942. This resulted in Synechococcus 7942 reduction in doubling time from 6.8 to 2.3 hours[5]

References

  1. 1 2 3 4 5 Yu J, Liberton M, Cliften PF, Head RD, Jacobs JM, Smith RD, et al. (January 2015). "Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO₂". Scientific Reports. 5 (1): 8132. Bibcode:2015NatSR...5E8132Y. doi:10.1038/srep08132. PMC 5389031. PMID 25633131.
  2. ↑ Kratz WA, Myers J (1955). "Nutrition and Growth of Several Blue-Green Algae". American Journal of Botany. 42 (3): 282. doi:10.2307/2438564. JSTOR 2438564.
  3. ↑ Ungerer, Justin; Lin, Po-Cheng; Chen, Hui-Yuan; Pakrasi, Himadri B. (2018-03-07). "Adjustments to Photosystem Stoichiometry and Electron Transfer Proteins Are Key to the Remarkably Fast Growth of the Cyanobacterium Synechococcus elongatus UTEX 2973". mBio. 9 (1). doi:10.1128/mBio.02327-17. ISSN 2150-7511. PMID 29437923.
  4. ↑ Racharaks, Ratanachat; Peccia, Jordan (August 2019). "Cryopreservation of Synechococcus elongatus UTEX 2973". Journal of Applied Phycology. 31 (4): 2267–2276. doi:10.1007/s10811-018-1714-9. ISSN 0921-8971.
  5. ↑ Ungerer J, Wendt KE, Hendry JI, Maranas CD, Pakrasi HB (December 2018). "Synechococcus elongatus UTEX 2973". Proceedings of the National Academy of Sciences of the United States of America. 115 (50): E11761–E11770. doi:10.1073/pnas.1814912115. PMC 6294925. PMID 30409802.
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