Transmission disequilibrium test

Description of the test

We first describe the TDT in the case where families consist of trios (two parents and one affected child). Our description follows the notations used in Spielman, McGinnis & Ewens (1993).^[1]

The TDT measures the over-transmission of an allele from heterozygous parents to affected offsprings. The n affected offsprings have 2n parents. These can be represented by the transmitted and the non-transmitted alleles ${\displaystyle M_{1}}$ and ${\displaystyle M_{2}}$ at some genetic locus. Summarizing the data in a 2 by 2 table gives:

The derivation of the TDT shows that one should only use the heterozygous parents (total number b+c). The TDT tests whether the proportions b/(b+c) and c/(b+c) are compatible with probabilities (0.5, 0.5). This hypothesis can be tested using a binomial (asymptotically chi-square) test with one degree of freedom:

${\displaystyle \chi ^{2}={\frac {[b-(b+c)/2]^{2}}{(b+c)/2}}+{\frac {[c-(b+c)/2]^{2}}{(b+c)/2}}={\frac {(b-c)^{2}}{b+c}}}$

Outline of the test derivation

A derivation of the test consists of using a population genetics model to obtain the expected proportions for the quantities ${\displaystyle a,b,c}$ and ${\displaystyle d}$ in the table above. In particular, one can show that under nearly all disease models the expected proportion of ${\displaystyle b}$ and ${\displaystyle c}$ are identical. This result motivates the use of a binomial (asymptotically ${\displaystyle \chi ^{2}}$) test to test whether these proportions are equal.

On the other hand, one can also show that under such models the proportions ${\displaystyle a,b,c}$ and ${\displaystyle d}$ are not equal to the product of the marginals probabilities ${\displaystyle (a+b)/2n}$, ${\displaystyle (c+d)/2n}$ and ${\displaystyle (a+c)/2n}$, ${\displaystyle (b+d)/2n}$. A rewording of this statement would be that the type of the transmitted allele is not, in general, independent of the type of the non-transmitted allele. A consequence is that a ${\displaystyle \chi ^{2}}$ test for homogeneity/independence does not test the appropriate hypothesis, and thus, only heterozygous parents are included.

Extension of the test

The TDT can be readily extended beyond the case of trios. We keep following the notations of Spielman, McGinnis & Ewens (1993).^[1] Consider a total of ${\displaystyle h}$ heterozygous parents. We use the fact that the transmission to different children are independent. The information can be then summarized in three categories:

${\displaystyle i}$ = number of parents who transmit ${\displaystyle M_{1}}$ to both children.
${\displaystyle h-i-j}$ = number of parents who transmit ${\displaystyle M_{1}}$ to one child and ${\displaystyle M_{2}}$ to another.
${\displaystyle j}$ = number of parents who transmit ${\displaystyle M_{2}}$ to both children.

Using the notations of the previous paragraph we have:

${\displaystyle b=2i+(h-i-j)=h+i-j\,}$

${\displaystyle c=2j+(h-i-j)=h-i+j\,}$

leading to the chi-squared test statistic:

${\displaystyle \chi _{tdt}^{2}={\frac {4(i-j)^{2}}{h}}.}$

Relation with another linkage statistic

The comparison with the more traditional (at least at the time when the TDT was proposed) linkage test proposed by Blackwelder and Elston 1985^[2] is informative. The Blackwelder and Elston approach uses the total number of haplotypes identical by descent (mean haplotype sharing). This measure ignores the allelic state of a marker and simply compares the number of times a parent transmits the same allele to both affected children with the number of times a different allele is transmitted. The test statistic is:

${\displaystyle \chi _{hs}^{2}={\frac {(2i+2j-h)^{2}}{h}}.}$

Under the null hypothesis of no linkage the expected proportions of (i, h − i − j, j) are (0.25, 0.5, 0.25). One can derive a simple chi-square statistic with 2 degrees of freedom:

${\displaystyle \chi _{total}^{2}={\frac {(i-h/4)^{2}}{h/4}}+{\frac {(h-i-j-h/2)^{2}}{h/2}}+{\frac {(j-h/4)^{2}}{h/4}}=\chi _{tdt}^{2}+\chi _{hs}^{2}.}$

It clearly appears that the total statistic (with two degree of freedom) is the sum of two independent components: one is the traditional linkage measure and the other is the TDT statistic.