Adenosylcobinamide-phosphate guanylyltransferase

In enzymology, an adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62) is an enzyme that catalyzes the chemical reaction

GTP + adenosylcobinamide phosphate diphosphate + adenosylcobinamide-GDP
adenosylcobinamide-phosphate guanylyltransferase
adenosylcobinamide phosphate guanylyltransferase (CobU) from salmonella typhimurium
Identifiers
EC no.2.7.7.62
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

The two substrates of this enzyme are GTP and adenosylcobinamide phosphate; its two products are diphosphate and adenosylcobinamide-GDP.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is GTP:adenosylcobinamide-phosphate guanylyltransferase. Other names in common use include CobU, adenosylcobinamide kinase/adenosylcobinamide-phosphate, guanylyltransferase, and AdoCbi kinase/AdoCbi-phosphate guanylyltransferase. This enzyme is part of the biosynthetic pathway to cobalamin (vitamin B12) in bacteria.

See also

References

    • O'Toole GA, Escalante-Semerena JC (1995). "Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate". J. Biol. Chem. 270 (40): 23560–9. doi:10.1074/jbc.270.40.23560. PMID 7559521.
    • Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I (1998). "Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 A resolution". Biochemistry. 37 (21): 7686–95. CiteSeerX 10.1.1.565.1238. doi:10.1021/bi973178f. PMID 9601028.
    • Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I (1999). "Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site". Biochemistry. 38 (40): 12995–3005. doi:10.1021/bi990910x. PMID 10529169.
    • Thomas MG, Thompson TB, Rayment I, Escalante-Semerena JC (2000). "Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation". J. Biol. Chem. 275 (36): 27576–86. doi:10.1074/jbc.M000977200. PMID 10869342.
    • Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002). "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19 (4): 390–412. doi:10.1039/b108967f. PMID 12195810.


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