Bis-tris methane
Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C. It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.[2]
Names | |
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Preferred IUPAC name
2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol[1] | |
Other names
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Identifiers | |
3D model (JSmol) |
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2205275 | |
ChEBI | |
ChemSpider | |
ECHA InfoCard | 100.027.489 |
EC Number |
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4519 | |
MeSH | Bistris |
PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C8H19NO5 | |
Molar mass | 209.242 g·mol−1 |
Appearance | White crystals |
Odor | Odourless |
Melting point | 102 to 103 °C (216 to 217 °F; 375 to 376 K) |
209.2 g L−1 (at 20 °C) | |
Acidity (pKa) | 6.46 |
Basicity (pKb) | 7.54 |
UV-vis (λmax) | 280 nm |
Absorbance | 0.15 |
Hazards | |
GHS labelling: | |
Warning | |
H315, H319, H335 | |
P261, P305+P351+P338 | |
NFPA 704 (fire diamond) | |
Related compounds | |
Related alkanols |
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Related compounds |
Diethylhydroxylamine |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references |
See also
References
- "Bistris - Compound Summary". PubChem Compound. USA: National Center for Biotechnology Information. 23 June 2005. Identification. Retrieved 9 April 2012.
- Ferreira, Carlos M. H.; Pinto, Isabel S. S.; Soares, Eduardo V.; Soares, Helena M. V. M. (2015). "(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review". RSC Advances. 5 (39): 30989–31003. doi:10.1039/c4ra15453c. hdl:10400.22/7404. ISSN 2046-2069.
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