Darlingtonia californica

Because many carnivorous species live in hostile environments, their root systems are commonly as highly modified as their leaves. Darlingtonia californica is no exception. The cobra lily has a very large and rambling root system when compared to those of other carnivorous plants in the family Sarraceniaceae.[5] The cobra lily is able to survive fire by regenerating from its roots, but despite this important role the roots are very delicate organs. While the temperatures in much of the species's range can exceed 25 °C (77 °F), their roots die back after exposure to temperatures not much higher than 10 °C (50 °F) . Temperature plays a large part in the functioning of all plants, but it is very rare for individual organs to have such different temperature tolerances. The physiological mechanisms and evolutionary benefits of this discrepancy are not fully understood, however, in habitat the plants are normally found growing out of cold seeps, and this behavior would cause the plant to only expend energy growing roots in the direction of cold subsurface water sources. The reason for this extraordinary sensitivity of the roots to high temperatures is currently believed to be caused by a very low and limited optimal temperature of the ion pumps in root cells [5][6]

Modern cultivation efforts in breeding and selecting plants that can withstand higher temperatures without the roots dying back have met with significant success. As a result, there are several cultivars available as commercial house or garden plants which are more tolerant of higher temperatures. Many wild populations grow in serpentine (ultramafic) soils which are toxic to most green plants. Populations found growing on serpentine can withstand soil temperatures above 80 °F based on field studies of various cobra lily populations in Oregon, most of which are found growing on slopes composed of serpentine with cold subsurface water flow. The plants can suffer from root rot in cultivation if grown in stagnant, standing water and tend to thrive in areas where cold subsurface water slowly flows underground around their roots. It is not currently understood why the plants can withstand higher soil temperatures when found growing on serpentine soils.[7][8]

The cobra lily is unique among the three genera of American pitcher plants. It does not trap rainwater in its pitcher. Instead, it regulates the level of water inside physiologically by releasing or absorbing water into the trap that has been pumped up from the roots. It was once believed that this species of pitcher plant did not produce any digestive enzymes and relied on symbiotic bacteria and protozoa to break down the captured insects into easily absorbed nutrients. However, recent studies have indicated that Darlingtonia secretes at least one proteolytic enzyme that digests captured prey.[9]^: 61 The cells that absorb nutrients from the inside of the pitcher are the same as those on the roots that absorb soil nutrients. The efficiency of the plant's trapping ability is attested to by its leaves and pitchers, which are, more often than not, full of insects and their remains.[9]^: 58

The slippery walls and hairs of the pitcher tube prevent trapped prey from escaping. In addition to the lubricating secretions and downward-pointing hairs common to all North American pitcher plants to force their prey into the trap, this species uses its curled operculum (hood) to hide the tiny exit hole from trapped insects and offers multiple translucent false exits. Upon trying many times to escape via the false exits, the insect will tire and fall down into the trap. Other species that use a low-hanging hood to hide the exit hole include the parrot pitcher plant, Sarracenia psittacina and the hooded pitcher plant, Sarracenia minor. S. psittacina also forms a curled operculum, while S. minor uses a leaf that is folded over the entrance. A misconception about Darlingtonia is that its forked tongue is an adaption to trap insects. However, a study done by American Journal of Botany determined that removal of the tongue does not affect prey biomass.[10]

A remaining mystery surrounding the cobra lily is its means of pollination. Its flower is unusually shaped and complex, typically a sign of a close pollinator-plant specialization. The flower is yellowish purple in color and grows on a stalk which is slightly taller than the pitcher leaves. It has five sepals, green in color, which are longer than the red-veined petals. It is generally expected that the pollinator is either a fly or bee attracted to the flower's unpleasant smell or some nocturnal insect.[11]

A new study suggests that it may be melittophilous after observing a miner bee species (Andrena nigrihirta). Pollinating by hand yielded poor results, therefore, melittophilous seems likely considering the complexity of the fruit. According to the study, "Observations of A. nigrihirta on flowers revealed that the shape and orientation of D. californica's ovary and petals promote stigma contact both when pollinators enter and exit a flower, contrary to previous thought. Our findings provide evidence that D. californica is melittophilous". Furthermore, Darlingtonia was pollen-limited in all five plants observed. However, in the case of male absence, Darlingtonia was still able to reproduce suggesting that self pollination may play a role as well.[12] It seems likely that both occur and only bolsters the reputation of hardiness in the wild.[13]