Curdlan

Curdlan is a water-insoluble linear beta-1,3-glucan, a high-molecular-weight polymer of glucose. Curdlan consists of β-(1,3)-linked glucose residues and forms elastic gels upon heating in aqueous suspension. It was reported to be produced by Alcaligenes faecalis var. myxogenes.[1] Subsequently, the taxonomy of this non-pathogenic curdlan-producing bacterium has been reclassified as Agrobacterium species.[2]

Curdlan
Identifiers
ChemSpider
  • none
E number E424 (thickeners, ...)
UNII
Properties
(C6H10O5)n
Appearance odourless white powder
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Extracellular and capsular polysaccharides are produced by a variety of pathogenic and soil-dwelling bacteria. Curdlan is a neutral β-(1,3)-glucan, perhaps with a few intra- or interchain 1,6-linkages, produced as an exopolysaccharide by soil bacteria of the family Rhizobiaceae.[3] Four genes required for curdlan production have been identified in Agrobacterium sp. ATCC31749, which produces curdlan in extraordinary amounts, and Agrobacterium tumefaciens.[4] A putative operon contains crdS (Q9X2V0, family GT2, Pfam PF13632), encoding β-(1,3)-glucan synthase catalytic subunit,[5] flanked by two additional genes. A separate locus contains a putative regulatory gene, crdR. A membrane-bound phosphatidylserine synthase, encoded by pssAG, is also necessary for maximal production of curdlan of high molecular mass. Nitrogen starvation upregulates the curdlan operon and increases the rate of curdlan synthesis.[6]

Curdlan has numerous applications as a gelling agent in the food, construction, and pharmaceutical industries and has been approved as a food additive by the U. S. Food and Drug Administration.[7] Its use is being evaluated in fat replacement studies in foodstuffs such as sausages, meat patties and other meat products [8]

See also

References
  1. Harada T., Fujimori K., Hirose S., Masada M. (1966). "Growth and Glucan (10C3K) Production by a Mutant of Alcaligenes faecalis var myxogenes in Defined Medium". Agric Biol Chem. 30: 764–769. doi:10.1271/bbb1961.30.764.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. Xiao-Bei Zhan, Chi-Chung Lin, Hong-Tao Zhang (2012). "Recent advances in curdlan biosynthesis, biotechnological production, and applications". Applied Microbiology and Biotechnology. 93 (2): 525–531. doi:10.1007/s00253-011-3740-2. PMID 22124723. S2CID 7185132.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  3. McIntosh M, Stone BA, Stanisich VA (2005). "Curdlan and other bacterial (1-->3)-beta-D-glucans". Appl Microbiol Biotechnol. 68 (2): 163–73. doi:10.1007/s00253-005-1959-5. PMID 15818477. S2CID 13123359.
  4. Karnezis T, Epa VC, Stone BA, Stanisich VA (2003). "Topological characterization of an inner membrane (1-->3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp. strain ATCC31749". Glycobiology. 13 (10): 693–706. doi:10.1093/glycob/cwg093. PMID 12851288.
  5. Karnezis, T. (2003-06-10). "Topological characterization of an inner membrane (1->3)- -D-glucan (curdlan) synthase from Agrobacterium sp. strain ATCC31749". Glycobiology. 13 (10): 693–706. doi:10.1093/glycob/cwg093. ISSN 1460-2423. PMID 12851288.
  6. Ruffing AM, Chen RR (February 2012). "Transcriptome profiling of a curdlan-producing Agrobacterium reveals conserved regulatory mechanisms of exopolysaccharide biosynthesis". Microb Cell Fact. 11: 17. doi:10.1186/1475-2859-11-17. PMC 3293034. PMID 22305302.
  7. "Compendium of Food Additive Specifications (Addendum 7) Joint FAO/WHO Expert. Curdlan: New specification prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999)".
  8. Aquinas, Natasha; Bhat M, Ramananda; Selvaraj, Subbalaxmi (2021-08-25). "A review presenting production, characterization, and applications of biopolymer curdlan in food and pharmaceutical sectors". Polymer Bulletin. 79 (9): 6905–6927. doi:10.1007/s00289-021-03860-1. ISSN 1436-2449. S2CID 237299620.
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