Meristem

The meristem is a type of tissue found in plants. It consists of undifferentiated cells (meristematic cells) capable of cell division. Cells in the meristem can develop into all the other tissues and organs that occur in plants. These cells continue to divide until a time when they get differentiated and then lose the ability to divide.

Tunica-corpus model of the apical meristem (growing tip). The epidermal (L1) and subepidermal (L2) layers form the outer layers called the tunica. The inner L3 layer is called the corpus. Cells in the L1 and L2 layers divide in a sideways fashion, which keeps these layers distinct, whereas the L3 layer divides in a more random fashion.

Differentiated plant cells generally cannot divide or produce cells of a different type. Meristematic cells are undifferentiated or incompletely differentiated. They are totipotent and capable of continued cell division. Division of meristematic cells provides new cells for expansion and differentiation of tissues and the initiation of new organs, providing the basic structure of the plant body. The cells are small, with small vacuoles or none, and protoplasm filling the cell completely. The plastids (chloroplasts or chromoplasts), are undifferentiated, but are present in rudimentary form (proplastids). Meristematic cells are packed closely together without intercellular spaces. The cell wall is a very thin primary cell wall.

The term meristem was first used in 1858 by Carl Wilhelm von Nägeli (1817–1891) in his book Beiträge zur Wissenschaftlichen Botanik ("Contributions to Scientific Botany").[1] It is derived from the Greek word merizein (μερίζειν), meaning to divide, in recognition of its inherent function.

There are three types of meristematic tissues: apical (at the tips), intercalary or basal (in the middle), and lateral (at the sides). At the meristem summit, there is a small group of slowly dividing cells, which is commonly called the central zone. Cells of this zone have a stem cell function and are essential for meristem maintenance. The proliferation and growth rates at the meristem summit usually differ considerably from those at the periphery.

Apical meristems

Apical meristems are the completely undifferentiated (indeterminate) meristems in a plant. These differentiate into three kinds of primary meristems. The primary meristems in turn produce the two secondary meristem types. These secondary meristems are also known as lateral meristems because they are involved in lateral growth.

Organisation of an apical meristem (growing tip)
  1. Central zone
  2. Peripheral zone
  3. Medullary (i.e. central) meristem
  4. Medullary tissue

There are two types of apical meristem tissue: shoot apical meristem (SAM), which gives rise to organs like the leaves and flowers, and root apical meristem (RAM), which provides the meristematic cells for future root growth. SAM and RAM cells divide rapidly and are considered indeterminate, in that they do not possess any defined end status. In that sense, the meristematic cells are frequently compared to the stem cells in animals, which have an analogous behavior and function.

The apical meristems are layered where the number of layers varies according to plant type. In general the outermost layer is called the tunica while the innermost layers are the corpus. In monocots, the tunica determines the physical characteristics of the leaf edge and margin. In dicots, layer two of the corpus determines the characteristics of the edge of the leaf. The corpus and tunica play a critical part of the plant physical appearance as all plant cells are formed from the meristems. Apical meristems are found in two locations: the root and the stem. Some Arctic plants have an apical meristem in the lower/middle parts of the plant. It is thought that this kind of meristem evolved because it is advantageous in Arctic conditions.

Shoot apical meristems
Shoot apical meristems of Crassula ovata (left). Fourteen days later, leaves have developed (right).

Shoot apical meristems are the source of all above-ground organs, such as leaves and flowers. Cells at the shoot apical meristem summit serve as stem cells to the surrounding peripheral region, where they proliferate rapidly and are incorporated into differentiating leaf or flower primordia.

The shoot apical meristem is the site of most of the embryogenesis in flowering plants. Primordia of leaves, sepals, petals, stamens, and ovaries are initiated here at the rate of one every time interval, called a plastochron. It is where the first indications that flower development has been evoked are manifested. One of these indications might be the loss of apical dominance and the release of otherwise dormant cells to develop as auxiliary shoot meristems, in some species in axils of primordia as close as two or three away from the apical dome.

The shoot apical meristem consists of four distinct cell groups:

  • Stem cells
  • The immediate daughter cells of the stem cells
  • A subjacent organizing center
  • Founder cells for organ initiation in surrounding regions

These four distinct zones are maintained by a complex signalling pathway. In Arabidopsis thaliana, 3 interacting CLAVATA genes are required to regulate the size of the stem cell reservoir in the shoot apical meristem by controlling the rate of cell division.[2] CLV1 and CLV2 are predicted to form a receptor complex (of the LRR receptor-like kinase family) to which CLV3 is a ligand.[3][4][5] CLV3 shares some homology with the ESR proteins of maize, with a short 14 amino acid region being conserved between the proteins.[6][7] Proteins that contain these conserved regions have been grouped into the CLE family of proteins.[6][7]

CLV1 has been shown to interact with several cytoplasmic proteins that are most likely involved in downstream signalling. For example, the CLV complex has been found to be associated with Rho/Rac small GTPase-related proteins.[2] These proteins may act as an intermediate between the CLV complex and a mitogen-activated protein kinase (MAPK), which is often involved in signalling cascades.[8] KAPP is a kinase-associated protein phosphatase that has been shown to interact with CLV1.[9] KAPP is thought to act as a negative regulator of CLV1 by dephosphorylating it.[9]

Another important gene in plant meristem maintenance is WUSCHEL (shortened to WUS), which is a target of CLV signaling in addition to positively regulating CLV, thus forming a feedback loop.[10] WUS is expressed in the cells below the stem cells of the meristem and its presence prevents the differentiation of the stem cells.[10] CLV1 acts to promote cellular differentiation by repressing WUS activity outside of the central zone containing the stem cells.[2]

The function of WUS in the shoot apical meristem is linked to the phytohormone cytokinin. Cytokinin activates histidine kinases which then phosphorylate histidine phosphotransfer proteins.[11] Subsequently, the phosphate groups are transferred onto two types of Arabidopsis response regulators (ARRs): Type-B ARRS and Type-A ARRs. Type-B ARRs work as transcription factors to activate genes downstream of cytokinin, including A-ARRs. A-ARRs are similar to B-ARRs in structure; however, A-ARRs do not contain the DNA binding domains that B-ARRs have, and which are required to function as transcription factors.[12] Therefore, A-ARRs do not contribute to the activation of transcription, and by competing for phosphates from phosphotransfer proteins, inhibit B-ARRs function.[13] In the SAM, B-ARRs induce the expression of WUS which induces stem cell identity.[14] WUS then suppresses A-ARRs.[15] As a result, B-ARRs are no longer inhibited, causing sustained cytokinin signaling in the center of the shoot apical meristem. Altogether with CLAVATA signaling, this system works as a negative feedback loop. Cytokinin signaling is positively reinforced by WUS to prevent the inhibition of cytokinin signaling, while WUS promotes its own inhibitor in the form of CLV3, which ultimately keeps WUS and cytokinin signaling in check.[16]

Root apical meristem
10x microscope image of root tip with meristem
  1. quiescent center
  2. calyptrogen (live rootcap cells)
  3. rootcap
  4. sloughed off dead rootcap cells
  5. procambium

Unlike the shoot apical meristem, the root apical meristem produces cells in two dimensions. It harbors two pools of stem cells around an organizing center called the quiescent center (QC) cells and together produces most of the cells in an adult root.[17][18] At its apex, the root meristem is covered by the root cap, which protects and guides its growth trajectory. Cells are continuously sloughed off the outer surface of the root cap. The QC cells are characterized by their low mitotic activity. Evidence suggests that the QC maintains the surrounding stem cells by preventing their differentiation, via signal(s) that are yet to be discovered. This allows a constant supply of new cells in the meristem required for continuous root growth. Recent findings indicate that QC can also act as a reservoir of stem cells to replenish whatever is lost or damaged.[19] Root apical meristem and tissue patterns become established in the embryo in the case of the primary root, and in the new lateral root primordium in the case of secondary roots.

Intercalary meristem

In angiosperms, intercalary (sometimes called basal) meristems occur in monocot (in particular, grass) stems at the base of nodes and leaf blades. Horsetails and Welwitschia also exhibit intercalary growth. Intercalary meristems are capable of cell division, and they allow for rapid growth and regrowth of many monocots. Intercalary meristems at the nodes of bamboo allow for rapid stem elongation, while those at the base of most grass leaf blades allow damaged leaves to rapidly regrow. This leaf regrowth in grasses evolved in response to damage by grazing herbivores.

Floral meristem
Further information: ABC model of flower development

When plants begin flowering, the shoot apical meristem is transformed into an inflorescence meristem, which goes on to produce the floral meristem, which produces the sepals, petals, stamens, and carpels of the flower.

In contrast to vegetative apical meristems and some efflorescence meristems, floral meristems cannot continue to grow indefinitely. Their growth is limited to the flower with a particular size and form. The transition from shoot meristem to floral meristem requires floral meristem identity genes, that both specify the floral organs and cause the termination of the production of stem cells. AGAMOUS (AG) is a floral homeotic gene required for floral meristem termination and necessary for proper development of the stamens and carpels.[2] AG is necessary to prevent the conversion of floral meristems to inflorescence shoot meristems, but is identity gene LEAFY (LFY) and WUS and is restricted to the centre of the floral meristem or the inner two whorls.[20] This way floral identity and region specificity is achieved. WUS activates AG by binding to a consensus sequence in the AG's second intron and LFY binds to adjacent recognition sites.[20] Once AG is activated it represses expression of WUS leading to the termination of the meristem.[20]

Through the years, scientists have manipulated floral meristems for economic reasons. An example is the mutant tobacco plant "Maryland Mammoth". In 1936, the department of agriculture of Switzerland performed several scientific tests with this plant. "Maryland Mammoth" is peculiar in that it grows much faster than other tobacco plants.

Apical dominance

Apical dominance is where one meristem prevents or inhibits the growth of other meristems. As a result, the plant will have one clearly defined main trunk. For example, in trees, the tip of the main trunk bears the dominant shoot meristem. Therefore, the tip of the trunk grows rapidly and is not shadowed by branches. If the dominant meristem is cut off, one or more branch tips will assume dominance. The branch will start growing faster and the new growth will be vertical. Over the years, the branch may begin to look more and more like an extension of the main trunk. Often several branches will exhibit this behavior after the removal of apical meristem, leading to a bushy growth.

The mechanism of apical dominance is based on auxins, types of plant growth regulators. These are produced in the apical meristem and transported towards the roots in the cambium. If apical dominance is complete, they prevent any branches from forming as long as the apical meristem is active. If the dominance is incomplete, side branches will develop.

Recent investigations into apical dominance and the control of branching have revealed a new plant hormone family termed strigolactones. These compounds were previously known to be involved in seed germination and communication with mycorrhizal fungi and are now shown to be involved in inhibition of branching.[21]

Diversity in meristem architectures

The SAM contains a population of stem cells that also produce the lateral meristems while the stem elongates. It turns out that the mechanism of regulation of the stem cell number might be evolutionarily conserved. The CLAVATA gene CLV2 responsible for maintaining the stem cell population in Arabidopsis thaliana is very closely related to the maize gene FASCIATED EAR 2(FEA2) also involved in the same function.[22] Similarly, in rice, the FON1-FON2 system seems to bear a close relationship with the CLV signaling system in Arabidopsis thaliana.[23] These studies suggest that the regulation of stem cell number, identity and differentiation might be an evolutionarily conserved mechanism in monocots, if not in angiosperms. Rice also contains another genetic system distinct from FON1-FON2, that is involved in regulating stem cell number.[23] This example underlines the innovation that goes about in the living world all the time.

Role of the KNOX-family genes
Note the long spur of the above flower. Spurs attract pollinators and confer pollinator specificity. (Flower: Linaria dalmatica)
Complex leaves of Cardamine hirsuta result from KNOX gene expression

Genetic screens have identified genes belonging to the KNOX family in this function. These genes essentially maintain the stem cells in an undifferentiated state. The KNOX family has undergone quite a bit of evolutionary diversification while keeping the overall mechanism more or less similar. Members of the KNOX family have been found in plants as diverse as Arabidopsis thaliana, rice, barley and tomato. KNOX-like genes are also present in some algae, mosses, ferns and gymnosperms. Misexpression of these genes leads to the formation of interesting morphological features. For example, among members of Antirrhineae, only the species of the genus Antirrhinum lack a structure called spur in the floral region. A spur is considered an evolutionary innovation because it defines pollinator specificity and attraction. Researchers carried out transposon mutagenesis in Antirrhinum majus, and saw that some insertions led to formation of spurs that were very similar to the other members of Antirrhineae,[24] indicating that the loss of spur in wild Antirrhinum majus populations could probably be an evolutionary innovation.

The KNOX family has also been implicated in leaf shape evolution (See below for a more detailed discussion). One study looked at the pattern of KNOX gene expression in A. thaliana, that has simple leaves and Cardamine hirsuta, a plant having complex leaves. In A. thaliana, the KNOX genes are completely turned off in leaves, but in C.hirsuta, the expression continued, generating complex leaves.[25] Also, it has been proposed that the mechanism of KNOX gene action is conserved across all vascular plants, because there is a tight correlation between KNOX expression and a complex leaf morphology.[26]

Primary meristems

Apical meristems may differentiate into three kinds of primary meristem:

  • Protoderm: lies around the outside of the stem and develops into the epidermis.
  • Procambium: lies just inside of the protoderm and develops into primary xylem and primary phloem. It also produces the vascular cambium, and cork cambium, secondary meristems. The cork cambium further differentiates into the phelloderm (to the inside) and the phellem, or cork (to the outside). All three of these layers (cork cambium, phellem, and phelloderm) constitute the periderm. In roots, the procambium can also give rise to the pericycle, which produces lateral roots in eudicots.[27]
  • Ground meristem: develops into the cortex and the pith. Composed of parenchyma, collenchyma and sclerenchyma cells.[27]

These meristems are responsible for primary growth, or an increase in length or height, which were discovered by scientist Joseph D. Carr of North Carolina in 1943.

Secondary meristems

There are two types of secondary meristems, these are also called the lateral meristems because they surround the established stem of a plant and cause it to grow laterally (i.e., larger in diameter).

  • Vascular cambium, which produces secondary xylem and secondary phloem. This is a process that may continue throughout the life of the plant. This is what gives rise to wood in plants. Such plants are called arboraceous. This does not occur in plants that do not go through secondary growth (known as herbaceous plants).
  • Cork cambium, which gives rise to the periderm, which replaces the epidermis.

Indeterminate growth of meristems
Further information: Root nodule

Though each plant grows according to a certain set of rules, each new root and shoot meristem can go on growing for as long as it is alive. In many plants, meristematic growth is potentially indeterminate, making the overall shape of the plant not determinate in advance. This is the primary growth. Primary growth leads to lengthening of the plant body and organ formation. All plant organs arise ultimately from cell divisions in the apical meristems, followed by cell expansion and differentiation. Primary growth gives rise to the apical part of many plants.

The growth of nitrogen-fixing root nodules on legume plants such as soybean and pea is either determinate or indeterminate. Thus, soybean (or bean and Lotus japonicus) produce determinate nodules (spherical), with a branched vascular system surrounding the central infected zone. Often, Rhizobium-infected cells have only small vacuoles. In contrast, nodules on pea, clovers, and Medicago truncatula are indeterminate, to maintain (at least for some time) an active meristem that yields new cells for Rhizobium infection. Thus zones of maturity exist in the nodule. Infected cells usually possess a large vacuole. The plant vascular system is branched and peripheral.

Cloning

Under appropriate conditions, each shoot meristem can develop into a complete, new plant or clone. Such new plants can be grown from shoot cuttings that contain an apical meristem. Root apical meristems are not readily cloned, however. This cloning is called asexual reproduction or vegetative reproduction and is widely practiced in horticulture to mass-produce plants of a desirable genotype. This process known as mericloning, has been shown to reduce or eliminate viruses present in the parent plant in multiple species of plants.[28][29]

Propagating through cuttings is another form of vegetative propagation that initiates root or shoot production from secondary meristematic cambial cells. This explains why basal 'wounding' of shoot-borne cuttings often aids root formation.[30]

Induced meristems

Meristems may also be induced in the roots of legumes such as soybean, Lotus japonicus, pea, and Medicago truncatula after infection with soil bacteria commonly called Rhizobia. Cells of the inner or outer cortex in the so-called "window of nodulation" just behind the developing root tip are induced to divide. The critical signal substance is the lipo-oligosaccharide Nod factor, decorated with side groups to allow specificity of interaction. The Nod factor receptor proteins NFR1 and NFR5 were cloned from several legumes including Lotus japonicus, Medicago truncatula and soybean (Glycine max). Regulation of nodule meristems utilizes long-distance regulation known as the autoregulation of nodulation (AON). This process involves a leaf-vascular tissue located LRR receptor kinases (LjHAR1, GmNARK and MtSUNN), CLE peptide signalling, and KAPP interaction, similar to that seen in the CLV1,2,3 system. LjKLAVIER also exhibits a nodule regulation phenotype though it is not yet known how this relates to the other AON receptor kinases.

See also

References
  1. Galun, Esra (2007). Plant Patterning: Structural and Molecular Genetic Aspects. World Scientific Publishing Company. p. 333. ISBN 9789812704085
  2. Fletcher, J. C. (2002). "Shoot and Floral Meristem Maintenance in Arabidopsis". Annu. Rev. Plant Biol. 53: 45–66. doi:10.1146/annurev.arplant.53.092701.143332. PMID 12221985.
  3. Clark SE, Williams RW, Meyerowitz E (1997). "The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis". Cell. 89 (4): 575–85. doi:10.1016/S0092-8674(00)80239-1. PMID 9160749. S2CID 15360609.
  4. Jeong S, Trotochaud AE, Clark S (1999). "The Arabidopsis CLAVATA2 gene encodes a receptor-like protein required for the stability of the CLAVATA1 receptor-like kinase". Plant Cell. 11 (10): 1925–33. doi:10.1105/tpc.11.10.1925. PMC 144110. PMID 10521522.
  5. Fletcher JC, Brand U, Running MP, Simon R, Meyerowitz EM (1999). "Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems". Science. 283 (5409): 1911–14. Bibcode:1999Sci...283.1911F. doi:10.1126/science.283.5409.1911. PMID 10082464.
  6. J. Mark Cock; Sheila McCormick (July 2001). "A Large Family of Genes That Share Homology with CLAVATA3". Plant Physiology. 126 (3): 939–942. doi:10.1104/pp.126.3.939. PMC 1540125. PMID 11457943.
  7. Karsten Oelkers, Nicolas Goffard, Georg F Weiller, Peter M Gresshoff, Ulrike Mathesius and Tancred Frickey (3 January 2008). "Bioinformatic Analysis of the CLE signalling peptide family". BMC Plant Biology. 8: 1. doi:10.1186/1471-2229-8-1. PMC 2254619. PMID 18171480.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. Valster, A. H.; et al. (2000). "Plant GTPases: the Rhos in bloom". Trends in Cell Biology. 10 (4): 141–146. doi:10.1016/s0962-8924(00)01728-1. PMID 10740268.
  9. Stone, J. M.; et al. (1998). "Control of meristem development by CLAVATA1 receptor kinase and kinase-associated protein phosphatase interactions". Plant Physiology. 117 (4): 1217–1225. doi:10.1104/pp.117.4.1217. PMC 34886. PMID 9701578.
  10. Mayer, K. F. X; et al. (1998). "Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem". Cell. 95 (6): 805–815. doi:10.1016/S0092-8674(00)81703-1. PMID 9865698. S2CID 18995751.
  11. Sheen, Jen; Hwang, Ildoo (September 2001). "Two-component circuitry in Arabidopsis cytokinin signal transduction". Nature. 413 (6854): 383–389. Bibcode:2001Natur.413..383H. doi:10.1038/35096500. ISSN 1476-4687. PMID 11574878. S2CID 4418158.
  12. Lohmann, Jan U.; Kieber, Joseph J.; Demar, Monika; Andreas Kehle; Stehling, Sandra; Busch, Wolfgang; To, Jennifer P. C.; Leibfried, Andrea (December 2005). "WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators". Nature. 438 (7071): 1172–1175. Bibcode:2005Natur.438.1172L. doi:10.1038/nature04270. ISSN 1476-4687. PMID 16372013. S2CID 2401801.
  13. Kieber, Joseph J.; Ecker, Joseph R.; Alonso, Jose M.; Schaller, G. Eric; Mason, Michael G.; Deruère, Jean; Ferreira, Fernando J.; Haberer, Georg; To, Jennifer P. C. (2004-03-01). "Type-A Arabidopsis Response Regulators Are Partially Redundant Negative Regulators of Cytokinin Signaling". The Plant Cell. 16 (3): 658–671. doi:10.1105/tpc.018978. ISSN 1040-4651. PMC 385279. PMID 14973166.
  14. Jurgens, G.; Berger, J.; Mayer, K. F.; Laux, T. (1996-01-01). "The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis". Development. 122 (1): 87–96. doi:10.1242/dev.122.1.87. ISSN 0950-1991. PMID 8565856.
  15. Jackson, David; Simon, Rüdiger; Je, Byoung Il; Somssich, Marc (2016-09-15). "CLAVATA-WUSCHEL signaling in the shoot meristem". Development. 143 (18): 3238–3248. doi:10.1242/dev.133645. ISSN 0950-1991. PMID 27624829.
  16. Gordon, S. P.; Chickarmane, V. S.; Ohno, C.; Meyerowitz, E. M. (2009-08-26). "Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem". Proceedings of the National Academy of Sciences. 106 (38): 16529–16534. Bibcode:2009PNAS..10616529G. doi:10.1073/pnas.0908122106. ISSN 0027-8424. PMC 2752578. PMID 19717465.
  17. Sebastian, Jose; Lee, Ji-Young (2013). "Root Apical Meristems". eLS. doi:10.1002/9780470015902.a0020121.pub2. ISBN 978-0470016176.
  18. Bennett, Tom; Scheres, Ben (2010). "Root development-two meristems for the price of one?". Current Topics in Developmental Biology. 91: 67–102. doi:10.1016/S0070-2153(10)91003-X. ISBN 9780123809100. PMID 20705179.
  19. Heidstra, Renze; Sabatini, Sabrina (2014). "Plant and animal stem cells: similar yet different". Nature Reviews Molecular Cell Biology. 15 (5): 301–12. doi:10.1038/nrm3790. PMID 24755933. S2CID 34386672.
  20. Lohmann, J. U. et al. (2001) A Molecular Link between Stem Cell Regulation and Floral Patterning in Arabidopsis Cell 105: 793-803
  21. "Branching out: new class of plant hormones inhibits branch formation". Nature. 455 (7210). 2008-09-11. Retrieved 2009-04-30.
  22. Taguchi-Shiobara; Yuan, Z; Hake, S; Jackson, D; et al. (2001). "The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize". Genes & Development. 15 (20): 2755–2766. doi:10.1101/gad.208501. PMC 312812. PMID 11641280.
  23. Suzaki T.; Toriba, T; Fujimoto, M; Tsutsumi, N; Kitano, H; Hirano, HY (2006). "Conservation and Diversification of Meristem Maintenance Mechanism in Oryza sativa: Function of the FLORAL ORGAN NUMBER2 Gene". Plant and Cell Physiol. 47 (12): 1591–1602. doi:10.1093/pcp/pcl025. PMID 17056620.
  24. Golz J.F.; Keck, Emma J.; Hudson, Andrew (2002). "Spontaneous Mutations in KNOX Genes Give Rise to a Novel Floral Structure in Antirrhinum". Curr. Biol. 12 (7): 515–522. doi:10.1016/S0960-9822(02)00721-2. PMID 11937019. S2CID 14469173.
  25. Hay and Tsiantis; Tsiantis, M (2006). "The genetic basis for differences in leaf form between Arabidopsis thaliana and its wild relative Cardamine hirsuta". Nat. Genet. 38 (8): 942–947. doi:10.1038/ng1835. PMID 16823378. S2CID 5775104.
  26. Bharathan G, et al. (2002). "Homologies in Leaf Form Inferred from KNOXI Gene Expression During Development". Science. 296 (5574): 1858–1860. Bibcode:2002Sci...296.1858B. doi:10.1126/science.1070343. PMID 12052958. S2CID 45069635.
  27. Evert, Ray, and Susan Eichhorn. Raven Biology of Plants. New York: W. H. Freeman and Company, 2013. Print.
  28. Adams, Alexa (April 2013). "Elimination of viruses from the hop (Humulus lupulus) by heat therapy and meristem culture". Journal of Horticultural Science. 50 (2): 151–160. doi:10.1080/00221589.1975.11514616. Retrieved 24 January 2023.
  29. Alam, I; Sharmin, SA; Naher, MK; Alam, MJ; Anisuzzaman, M; Alam, MF (April 2013). "Elimination and detection of viruses in meristem-derived plantlets of sweetpotato as a low-cost option toward commercialization". 3 Biotech. 3 (2): 53–164. doi:10.1007/s13205-012-0080-6. PMC 3597136. PMID 8324570.
  30. Mackenzie, K.A.D; Howard, B.H (1986). "The Anatomical Relationship Between Cambial Regeneration and Root Initiation in Wounded Winter Cuttings of the Apple Rootstock M.26". Annals of Botany. 58 (5): 649–661. doi:10.1093/oxfordjournals.aob.a087228.

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