J chain
The Joining (J) chain is a protein component that links monomers of antibodies IgM and IgA to form polymeric antibodies capable of secretion.[5] The J chain is well conserved in the animal kingdom, but its specific functions are yet to be fully understood. It is a 137 residue polypeptide,[6] encoded by the IGJ gene.[7][8][9]
1: Base unit.
2: Heavy chains.
3: Light chains.
4: J chain.
5: Intermolecular disulfide bonds.
Structure
The J chain is a glycoprotein of molecular weight 15 kDa. Its secondary structure remains undetermined but is believed to adopt either a single β-barrel or two-domain folded structure with standard immunoglobulin domains.[10] The J chain's primary structure is unusually acidic having a high content of negatively charged amino acids.[11] It has 8 cysteine residues, 6 of which are involved in intramolecular disulfide bonds while the remaining two function to bind the Fc tailpiece regions of IgA or IgM antibodies, the α chain and μ chain respectively. An N-linked carbohydrate resulting from N-glycosylation is also essential in the protein's incorporation to antibody polymers.[12] There is no known protein family with significant homology to the J chain.[13]
Function
Antibody polymerization
The J chain regulates the multimerization of IgM and IgA in mammals. When expressed in cells, it favors the formation of a pentameric IgM and an IgA dimer. IgM pentamers are most commonly found with a single J chain, but some studies have seen as many as 4 J chains associated to a single IgM pentamer.
The J chain is incorporated late in the formation of IgM polymers and thermodynamically favors the formation of pentamers as opposed to hexamers.[12] In J chain-knockout (KO) mice, the hexameric IgM polymer dominates.[14] These J chain negative IgM hexamers are 15-20 times more effective at activating complement than J chain positive IgM pentamers.[15] However, J chain-KO mice have been shown have low concentrations of hexameric IgM and a deficiency in complement activation, suggesting additional in vivo regulatory mechanisms.[16] Another consequence of pentameric IgM reduced complement activation is its allowance of J chain positive pIgM to bind antigen without causing excessive damage to epithelial membranes through complement activation.[17]
The J chain facilitates IgA dimerization by linking two monomer secretory tails. Structurally, the J chain joins two antibody monomers asymmetrically by forming intermolecular disulfide bonds and bringing hydrophobic β-sandwiches on each molecule together.[18] This multimerization mechanism involves chaperone proteins including binding immunoglobulin protein (BiP) and MZB1 each sequentially recruiting distinct factors of the polymerized antibody.[19]
Antibody secretion
Mucosal membrane antibody secretion from the basal membrane to apical epithelial cells is facilitated by the polymeric Ig receptor (pIgR). A basal protein of the pIgR known as secretory component (SC) recognizes Ig ready for secretion.[20] The binding between the secretory component and secretory Ig is facilitated by the antibody's J chain which makes physical contact with the secretory component in order to change the transporter's conformation to an open state.[21] The complex is then transcytosed and the secretory component proteolytically cleaved from the receptor releasing the antibody to the apical side of the epithelial cell and to the lumen at large. This mechanism is thought to be largely conserved between the secretion of IgM and IgA.[19]
Regulation
J chain was originally believed to only be expressed in antibody-secreting plasma cells, however, the J chain has been seen to be expressed in earlier stages of B cell differentiation prior to Ig expression.[22] J chain expression is believed to occur in the early stages of lymphoid cell differentiation as it is expressed in both B and T cell precursors. As cells develop, it seems that expression of the μ-chain becomes necessary for J chain synthesis.[23]
The J chain gene is transcriptionally regulated through canonical Pax5 repression.[24] As Pax5 is a common transcriptional regulator, the J chain is still expressed in plasma cells that secrete monomeric antibodies. In such cells it is believed to provide no function and is quickly degraded.[19] In plasma cells that secrete monomeric IgA, a Pax5-independent mechanism is likely to prevent IgA dimerization.[25]
Phylogeny
The J chain is likely to have evolutionarily arisen in early jaw-boned vertebrates.[26] Groups of bony fish including teleosts have since lost J chain expression.[13] Xenopus are able to polymerize mucosal IgX in the absence of J chain, perhaps due to a loss of the conserved cysteine residues that link the J chain and Ig secretory tail.[27]
Sharks do not express IgA and thus use J chain expression solely for the polymerization of IgM.[28] This makes sharks an intriguing model organism in studying J chain regulation and polymerization without the confounding variables of mucosal secretion.[29]
References
- GRCh38: Ensembl release 89: ENSG00000132465 - Ensembl, May 2017
- GRCm38: Ensembl release 89: ENSMUSG00000067149 - Ensembl, May 2017
- "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- Levinson. Medical Microbiology and Immunology (11 ed.). McGraw Hill. pp. 405–6.
- Schroeder H, Wald D, Greenspan N (2008). "Chapter 4: Immunoglobulins: Structure and Function". In Paul W (ed.). Fundamental Immunology (Book) (6th ed.). Philadelphia, PA: Lippincott Williams & Wilkins. pp. 125–151. ISBN 978-0-7817-6519-0.
- Max EE, McBride OW, Morton CC, Robinson MA (August 1986). "Human J chain gene: chromosomal localization and associated restriction fragment length polymorphisms". Proceedings of the National Academy of Sciences of the United States of America. 83 (15): 5592–5596. Bibcode:1986PNAS...83.5592M. doi:10.1073/pnas.83.15.5592. PMC 386334. PMID 3016707.
- Max EE, Korsmeyer SJ (April 1985). "Human J chain gene. Structure and expression in B lymphoid cells". The Journal of Experimental Medicine. 161 (4): 832–849. doi:10.1084/jem.161.4.832. PMC 2189063. PMID 2984306.
- "Entrez Gene: IGJ immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides".
- Frutiger S, Hughes GJ, Paquet N, Lüthy R, Jaton JC (December 1992). "Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M". Biochemistry. 31 (50): 12643–12647. doi:10.1021/bi00165a014. PMID 1472500.
- Klimovich VB, Samoĭlovich MP, Klimovich BV (2008-04-01). "[Problem of J-chain of immunoglobulins]". Zhurnal Evoliutsionnoi Biokhimii I Fiziologii. 44 (2): 131–143. doi:10.1134/S0022093008020023. PMID 18669274. S2CID 20628313.
- Sørensen V, Rasmussen IB, Sundvold V, Michaelsen TE, Sandlie I (January 2000). "Structural requirements for incorporation of J chain into human IgM and IgA". International Immunology. 12 (1): 19–27. doi:10.1093/intimm/12.1.19. PMID 10607746.
- Castro CD, Flajnik MF (October 2014). "Putting J chain back on the map: how might its expression define plasma cell development?". Journal of Immunology. 193 (7): 3248–3255. doi:10.4049/jimmunol.1400531. PMC 4198949. PMID 25240020.
- Davis AC, Roux KH, Shulman MJ (July 1988). "On the structure of polymeric IgM". European Journal of Immunology. 18 (7): 1001–1008. doi:10.1002/eji.1830180705. PMID 3136022. S2CID 34679165.
- Johansen FE, Braathen R, Brandtzaeg P (September 2000). "Role of J chain in secretory immunoglobulin formation". Scandinavian Journal of Immunology. 52 (3): 240–248. doi:10.1046/j.1365-3083.2000.00790.x. PMID 10972899. S2CID 5958810.
- Erlandsson L, Andersson K, Sigvardsson M, Lycke N, Leanderson T (August 1998). "Mice with an inactivated joining chain locus have perturbed IgM secretion". European Journal of Immunology. 28 (8): 2355–2365. doi:10.1002/(SICI)1521-4141(199808)28:08<2355::AID-IMMU2355>3.0.CO;2-L. PMID 9710213. S2CID 21659055.
- Grubb AO (2009-04-24). "Quantitation of J chain in human biological fluids by a simple immunochemical procedure". Acta Medica Scandinavica. 204 (6): 453–465. doi:10.1111/j.0954-6820.1978.tb08473.x. PMID 104551.
- Kumar N, Arthur CP, Ciferri C, Matsumoto ML (February 2020). "Structure of the secretory immunoglobulin A core". Science. 367 (6481): 1008–1014. Bibcode:2020Sci...367.1008K. doi:10.1126/science.aaz5807. PMID 32029686. S2CID 211050348.
- Wei H, Wang JY (February 2021). "Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis". International Journal of Molecular Sciences. 22 (5): 2284. doi:10.3390/ijms22052284. PMC 7956327. PMID 33668983.
- Stadtmueller BM, Huey-Tubman KE, López CJ, Yang Z, Hubbell WL, Bjorkman PJ (March 2016). Kuriyan J (ed.). "The structure and dynamics of secretory component and its interactions with polymeric immunoglobulins". eLife. 5: e10640. doi:10.7554/eLife.10640. PMC 4786434. PMID 26943617.
- Braathen R, Hohman VS, Brandtzaeg P, Johansen FE (February 2007). "Secretory antibody formation: conserved binding interactions between J chain and polymeric Ig receptor from humans and amphibians". Journal of Immunology. 178 (3): 1589–1597. doi:10.4049/jimmunol.178.3.1589. PMID 17237408. S2CID 27721152.
- Mestecky J, Fultz PN (May 1999). "Mucosal immune system of the human genital tract". The Journal of Infectious Diseases. 179 (Suppl 3): S470–S474. doi:10.1086/314806. JSTOR 30114178. PMID 10099122.
- Max EE, Korsmeyer SJ (April 1985). "Human J chain gene. Structure and expression in B lymphoid cells". The Journal of Experimental Medicine. 161 (4): 832–849. doi:10.1084/jem.161.4.832. PMC 2189063. PMID 2984306.
- Rao S, Karray S, Gackstetter ER, Koshland ME (October 1998). "Myocyte enhancer factor-related B-MEF2 is developmentally expressed in B cells and regulates the immunoglobulin J chain promoter". The Journal of Biological Chemistry. 273 (40): 26123–26129. doi:10.1074/jbc.273.40.26123. PMID 9748293.
- Hajdu I, Moldoveanu Z, Cooper MD, Mestecky J (December 1983). "Ultrastructural studies of human lymphoid cells. mu and J chain expression as a function of B cell differentiation". The Journal of Experimental Medicine. 158 (6): 1993–2006. doi:10.1084/jem.158.6.1993. PMC 2187181. PMID 6417260.
- Marchalonis J, Edelman GM (September 1965). "Phylogenetic origins of antibody structure. I. Multichain structure of immunoglobulins in the smooth dogfish (Mustelus canis)". The Journal of Experimental Medicine. 122 (3): 601–618. doi:10.1084/jem.122.3.601. PMC 2138074. PMID 4158437.
- Robert J, Ohta Y (June 2009). "Comparative and developmental study of the immune system in Xenopus". Developmental Dynamics. 238 (6): 1249–1270. doi:10.1002/dvdy.21891. PMC 2892269. PMID 19253402.
- Clem IW, De Boutaud F, Sigel MM (December 1967). "Phylogeny of immunoglobulin structure and function. II. Immunoglobulins of the nurse shark". Journal of Immunology. 99 (6): 1226–1235. doi:10.4049/jimmunol.99.6.1226. PMID 4168665.
- Hohman VS, Stewart SE, Rumfelt LL, Greenberg AS, Avila DW, Flajnik MF, Steiner LA (June 2003). "J chain in the nurse shark: implications for function in a lower vertebrate". Journal of Immunology. 170 (12): 6016–6023. doi:10.4049/jimmunol.170.12.6016. PMID 12794129. S2CID 292191.
Further reading
- Koshland ME (1986). "The coming of age of the immunoglobulin J chain". Annual Review of Immunology. 3: 425–453. doi:10.1146/annurev.iy.03.040185.002233. PMID 2415140.
- Tartakoff A, Vassalli P (November 1979). "Plasma cell immunoglobulin M molecules. Their biosynthesis, assembly, and intracellular transport". The Journal of Cell Biology. 83 (2 Pt 1): 284–299. doi:10.1083/jcb.83.2.284. PMC 2111544. PMID 115892.
- Mole JE, Bhown AS, Bennett JC (August 1977). "Primary structure of human J chain: alignment of peptides from chemical and enzymatic hydrolyses". Biochemistry. 16 (16): 3507–3513. doi:10.1021/bi00635a002. PMID 407930.
- Bastian A, Kratzin H, Eckart K, Hilschmann N (December 1992). "Intra- and interchain disulfide bridges of the human J chain in secretory immunoglobulin A". Biological Chemistry Hoppe-Seyler. 373 (12): 1255–1263. doi:10.1515/bchm3.1992.373.2.1255. PMID 1292512.
- Frutiger S, Hughes GJ, Paquet N, Lüthy R, Jaton JC (December 1992). "Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M". Biochemistry. 31 (50): 12643–12647. doi:10.1021/bi00165a014. PMID 1472500.
- Moro I, Iwase T, Komiyama K, Moldoveanu Z, Mestecky J (April 1990). "Immunoglobulin A (IgA) polymerization sites in human immunocytes: immunoelectron microscopic study". Cell Structure and Function. 15 (2): 85–91. doi:10.1247/csf.15.85. PMID 2113434.
- Alberini CM, Bet P, Milstein C, Sitia R (October 1990). "Secretion of immunoglobulin M assembly intermediates in the presence of reducing agents". Nature. 347 (6292): 485–487. Bibcode:1990Natur.347..485A. doi:10.1038/347485a0. PMID 2120591. S2CID 4348113.
- Sumi Y, Nagura H, Kaneda T, Oka T (September 1988). "Immunoelectron microscopical localization of immunoglobulins, secretory component and J chain in the human minor salivary glands". Journal of Oral Pathology. 17 (8): 390–395. doi:10.1111/j.1600-0714.1988.tb01303.x. PMID 3146624.
- Hajdu I, Moldoveanu Z, Cooper MD, Mestecky J (December 1983). "Ultrastructural studies of human lymphoid cells. mu and J chain expression as a function of B cell differentiation". The Journal of Experimental Medicine. 158 (6): 1993–2006. doi:10.1084/jem.158.6.1993. PMC 2187181. PMID 6417260.
- Yasuda N, Kanoh T, Uchino H (June 1980). "J chain synthesis in human myeloma cells: light and electron microscopic studies". Clinical and Experimental Immunology. 40 (3): 573–580. PMC 1538946. PMID 6774844.
- Harper SJ, Allen AC, Béné MC, Pringle JH, Faure G, Lauder I, Feehally J (September 1995). "Increased dimeric IgA-producing B cells in tonsils in IgA nephropathy determined by in situ hybridization for J chain mRNA". Clinical and Experimental Immunology. 101 (3): 442–448. doi:10.1111/j.1365-2249.1995.tb03132.x. PMC 1553245. PMID 7664491.
- Iwase T, Saito I, Takahashi T, Chu L, Usami T, Mestecky J, Moro I (October 1993). "Early expression of human J chain and mu chain gene in the fetal liver". Cell Structure and Function. 18 (5): 297–302. doi:10.1247/csf.18.297. PMID 8168154.
- Harper SJ, Pringle JH, Wicks AC, Hattersley J, Layward L, Allen A, et al. (March 1994). "Expression of J chain mRNA in duodenal IgA plasma cells in IgA nephropathy". Kidney International. 45 (3): 836–844. doi:10.1038/ki.1994.110. PMID 8196286.
- Bjercke S, Brandtzaeg P (September 1993). "Glandular distribution of immunoglobulins, J chain, secretory component, and HLA-DR in the human endometrium throughout the menstrual cycle". Human Reproduction. 8 (9): 1420–1425. doi:10.1093/oxfordjournals.humrep.a138271. PMID 8253928.
- Bertrand FE, Billips LG, Gartland GL, Kubagawa H, Schroeder HW (June 1996). "The J chain gene is transcribed during B and T lymphopoiesis in humans". Journal of Immunology. 156 (11): 4240–4244. doi:10.4049/jimmunol.156.11.4240. PMID 8666793. S2CID 44655675.
- Atkin JD, Pleass RJ, Owens RJ, Woof JM (July 1996). "Mutagenesis of the human IgA1 heavy chain tailpiece that prevents dimer assembly". Journal of Immunology. 157 (1): 156–159. doi:10.4049/jimmunol.157.1.156. PMID 8683109. S2CID 26899100.