Persephonella marina

Persephonella marina was first isolated in 1999 using MSH medium, a medium containing 29 g NaCl, 2 g NaOH, 0.5 g KCl, 1.36 g MgCl₂•6H₂O, 7 g MgSO₄•7H2O, 2 g Na₂S₂O₃•5H₂O, 0.4 g CaCl₂•2H₂O, 0.2 g NH₄Cl, 0.3 g K₂HPO₄•3H₂O and 10 ml of a trace-element stock solution, with a gas phase containing twenty parts carbon dioxide, one part oxygen, and twenty six parts hydrogen gas.[1] Initial isolation was provided by a sample obtained from a depth of 2,507 meters on a sulfidic chimney. This particular sulfidic chimney was named "Q-Vent" and is located at a latitude of 9° North and a longitude of 104° West in a region called the East Pacific Rise.[1] The environment in which P. marina was obtained was too harsh for humans due to the excessive temperature (133 °C with spikes up to 170 °C) and extreme pressure. Due to these harsh conditions a submarine was used for extraction of the samples.[1]

Persephonella marina has a genome size of 1.9 mega (10^9) base pairs with 2,048 encoded genes. The organism contains a GC content of 37%.[2] This is unusually low for thermophilic organisms which typically contain high amounts of GC bonds to prevent DNA denaturation. The organism's closest phylogenetic neighbor was isolated under the same study and was named Persephonella guaymasensis. It shares 96% of its genome with P. marina.[1] Other similar genomes include: Hydrogenothermus marinus (94.5% similarity),[1] and Aquifex pyrophilius (85% similarity).[1]

Persephonella marina was used as a model organism for the characterization of genes and enzymes for the synthesis of glucosylglycerate found for the first time in a thermophile.[3] Glucosylglycerate protects the microbe from thermal stresses and helps in adaptation to starvation conditions.[4] This is of great importance to scientists who wish to study extremophiles. This solute is also important in the advancement of biotechnology.[4] When tested in lab, glucosylglycerate increased the melting temperature of essential enzymes in basic metabolic pathways.[4] It has an effect on a microbe's ability to withstand high-pressure environments.[4] This rare solute has only been found in a few other halophilic bacteria and one Archaeon, but has never been encountered in a hyperthermophile such as P. marina.[4] It is also worth noting that P. marina has led to the discovery of α(1,6)glucosyl-α-(1,2)glucosylglycerate by proton NMR and is still currently under study.[4] Both of these solutes are disaccharide heterosides which are extremely rare in thermophiles.[4] Both are important in osmotic adaptation in microbes as well.[4] With many questions to be answered about these two solutes, further research could benefit biotechnology in application to production of things such as food preservatives and textiles. Also, for scientists who are looking to further answer why and how hyperthermophiles survive, these two solutes could help fill in the missing pieces of the puzzle.

• Type strain of Persephonella marina at BacDive - the Bacterial Diversity Metadatabase