REV1

DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene.[5][6]

REV1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesREV1, REV1L, AIBP80, DNA directed polymerase, REV1 DNA directed polymerase
External IDsOMIM: 606134 MGI: 1929074 HomoloGene: 32309 GeneCards: REV1
Orthologs
SpeciesHumanMouse
Entrez

51455

56210

Ensembl

ENSG00000135945

ENSMUSG00000026082

UniProt

Q9UBZ9

Q920Q2

RefSeq (mRNA)

NM_019570
NM_001359287
NM_001359288

RefSeq (protein)

NP_062516
NP_001346216
NP_001346217

Location (UCSC)Chr 2: 99.4 – 99.49 MbChr 1: 38.05 – 38.13 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.[6]

Rev1 is a Y family DNA polymerase; it is sometimes referred to as a deoxycytidyl transferase because it only inserts deoxycytidine (dC) across from lesions. Whether G, A, T, C, or an abasic site, Rev1 will always add a C. Rev1 has the ability to always add a C, because it uses an arginine as a template which complements well with C.[7] Yet it is believed that Rev1 rarely uses its polymerase activity; rather it is thought that Rev1's primary role is as a protein landing pad, whereby it helps direct the recruitment of TLS proteins, especially Pol ζ (Rev3/Rev7).

Interactions

REV1 has been shown to interact with MAD2L2.[8] It is believed that Rev1 may interact with PCNA, once ubiquitylated due to a lesion, and help recruit Pol ζ (Rev3/Rev7) a B family polymerase involved in TLS.

References

  1. GRCh38: Ensembl release 89: ENSG00000135945 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000026082 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Lin W, Xin H, Zhang Y, Wu X, Yuan F, Wang Z (Dec 1999). "The human REV1 gene codes for a DNA template-dependent dCMP transferase". Nucleic Acids Res. 27 (22): 4468–75. doi:10.1093/nar/27.22.4468. PMC 148731. PMID 10536157.
  6. "Entrez Gene: REV1 REV1 homolog (S. cerevisiae)".
  7. Nair, DT (Sep 30, 2005). "Rev1 employs a novel mechanism of DNA synthesis using a protein template". Science. 309 (5744): 2219–22. Bibcode:2005Sci...309.2219N. doi:10.1126/science.1116336. PMID 16195463. S2CID 35378034.
  8. Murakumo, Y; Ogura Y; Ishii H; Numata S; Ichihara M; Croce C M; Fishel R; Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. United States. 276 (38): 35644–51. doi:10.1074/jbc.M102051200. ISSN 0021-9258. PMID 11485998.

Further reading

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