Sakaguchi test

The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.[1] The Sakaguchi reagent used in the test consists of 1-Naphthol and a drop of sodium hypobromite. The guanidino (–C group in arginine reacts with the Sakaguchi reagent to form a red-coloured complex.[2][3][4][5]

References
  1. Sakaguchi, Shoyo (April 1925). "Über eine neue Farbenreaktion von protein und arginin" [About a new colorimetric reaction of protein and arginine]. Journal of Biochemistry (in German). Oxford University Press; Japanese Biochemical Society. 5: 25–31. doi:10.1093/oxfordjournals.jbchem.a128359.
  2. Chatterjea (1 January 2004). Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. Jaypee Brothers Publishers. p. 51. ISBN 978-81-8061-204-6.
  3. R.A. Joshi (2006). Question Bank of Biochemistry. New Age International. p. 64. ISBN 978-81-224-1736-4.
  4. Dandekar (1 January 2004). Practicals And Viva In Medical Biochemistry. Elsevier India. p. 28. ISBN 978-81-8147-025-6.
  5. Joshi, Rashmi A.; Marks, Victor (30 June 2004). Guide to Bio-chemistry. B. Jain Publishers. p. 74. ISBN 9788180560538.


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