TRNA pseudouridine32 synthase
tRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- tRNA uridine32 tRNA pseudouridine32
| tRNA pseudouridine32 synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 5.4.99.28 | ||||||||
| CAS no. | 430429-15-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA.
References
- Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR (November 2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Molecular Cell. 24 (4): 535–45. doi:10.1016/j.molcel.2006.09.017. PMID 17188032.
- Spedaliere CJ, Hamilton CS, Mueller EG (August 2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry. 39 (31): 9459–65. doi:10.1021/bi001079n. PMID 10924141.
- Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J (July 1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". The Journal of Biological Chemistry. 274 (27): 18880–6. doi:10.1074/jbc.274.27.18880. PMID 10383384.
- Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG (October 1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry. 38 (40): 13106–11. doi:10.1021/bi9913911. PMID 10529181.
- Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J (June 1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe)". RNA. 1 (4): 437–48. PMC 1482406. PMID 7493321.
- Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C (December 2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae" (PDF). The Journal of Biological Chemistry. 279 (51): 52998–3006. doi:10.1074/jbc.m409581200. PMID 15466869. S2CID 13197875.
External links
- TRNA+pseudouridine32+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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