Testosterone 17beta-dehydrogenase
In enzymology, a testosterone 17beta-dehydrogenase is an enzyme that catalyzes the chemical reaction between testosterone and androst-4-ene-3,17-dione. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
| testosterone 17-beta-dehydrogenase (NAD+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.63 | ||||||||
| CAS no. | 9028-62-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| testosterone 17-beta-dehydrogenase (NADP+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.64 | ||||||||
| CAS no. | 9028-63-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The systematic name of this enzyme class is 17beta-hydroxysteroid:NAD+ 17-oxidoreductase. Other names in common use include 17-ketoreductase and 17beta-HSD. This enzyme participates in androgen and estrogen metabolism.
Variants
There are two variants of the enzyme, one that uses NAD+ as a substrate, and one that uses NADP+ as acceptor.
NAD+
This variant of testosterone 17beta-dehydrogenase (EC 1.1.1.239) catalyzes the reaction
- testosterone + NAD+ androst-4-ene-3,17-dione + NADH + H+
Thus, the two substrates of this enzyme are testosterone and NAD+, whereas its 3 products are androst-4-ene-3,17-dione, NADH, and H+.
NADP+
This variant of testosterone 17beta-dehydrogenase (EC 1.1.1.64) catalyzes the reaction
- testosterone + NADP+ androst-4-ene-3,17-dione + NADPH + H+
Thus, the two substrates of this enzyme are testosterone and NADP+, whereas its 3 products are androst-4-ene-3,17-dione, NADPH, and H+.
References
- Endahl GL, Kochakian CD, Hamm D (1960). "Separation of a Triphosphopyridine Nucleotide-specific from a Diphosphopyridine Nucleotide-specific 17β-Hydroxy-(testosterone)dehydrogenase of Guinea Pig Liver". J. Biol. Chem. 235 (10): 2792–6. doi:10.1016/S0021-9258(18)64542-9. PMID 13696735.
- Sweat ML, Samuels LT, Lumry R (1950). "Preparation and characterization of the enzyme which converts testosterone to androstenedione". J. Biol. Chem. 185 (1): 75–84. doi:10.1016/S0021-9258(18)56395-X. PMID 15436478.
- Villee CA; Spencer JM (1960). "Some Properties of the Pyridine Nucleotide-specific 17β-Hydroxy Steroid Dehydrogenases of Guinea Pig Liver". J. Biol. Chem. 235 (12): 3615–3619. doi:10.1016/S0021-9258(18)64518-1. PMID 13781425.