Ceratiomyxa

Ceratiomyxa comes from the Latin word ceratus meaning "waxed" and the ancient Greek word myxa meaning "mucus".

Ceratiomyxa was first described under the name Puccinia ramose (later revised to Ceratiomyxa fruticulosa) in 1729 by Pier Antonio Micheli, a pioneer of mycology. In 1805, Albertini and Schweinitz described 2 more species in the genus, C. hydnoides and C. porioides. However, because Ceratiomyxa species can take a variety of different forms, many forms first described as species are later considered synonyms. This includes C. hydnoideum and C. porioides which are now often considered synonyms of C.fruticulosa.^1,2

Ceratiomyxa was first placed in the subclass Ceratiomyxomycetidae of the class Myxomycetes by Martin and Alexopoulos in 1969, but was later moved to subclass Protostelia of the class Eumycetozoa by Olive in 1970.^1,2

Ceratiomyxa is commonly found on rotting wood. Large logs and stumps are cited as ideal substrates for growth, although smaller colonies can also be found a tree branches. The Ceratiomyxa collection of Henry C. Gilbert has specimen growing on various evergreen coniferous trees (Pseudotsuga), elm (Ulmus), maple (Acer), oak (Quercus), Tilia, and willows (Salix). A specimen collected by F. O. Grover was found growing on a burlap sack.¹

Species of Ceratiomyxa can be found world-wide. C. fruticulosa is the most common species and has a cosmopolitan distribution. C. morchella and C. sphaerasperma have only been recorded in the tropics.³

Plasmodium: Net-like or thin layer enveloped in mucous. Often translucent or white in colour but can also be tinted slightly yellow, pink, or blue-green. The protoplasm within the plasmodium can be seen flowing, resting, and resuming flow in the opposite direction. This occurs in intervals of approximately 40 seconds. Protoplasmic flow slows as the sporangia develop and halt completely during mitosis.¹

Sporangia: Can be found in many different forms. Common forms include:

• Simple pillar: Pillar-like projections. In some species, pillars expand at the base and fuse to form a layer of plasmodium
• Fruticulus: Clusters of finger-like projections that are usually undivided¹
• Arbuscula: Branched resembling small trees. Spores develop on branch surfaces
• Filiforme: Long and slender pillars
• Porioides: Net-like formations of walls. Spores develop on sides and edges of wall

Spores: The spores are round or oval globules individually attached to stalks sprouting from sporangia. Spores vary in size (8-13 µm in diameter). The spore walls are thin and transparent. Spores appear grainy and may have some vacuoles which are often near the perimeter or surrounding the nucleus. The nucleus within the spore is about 3 µ in diameter.¹

Swarmcells: Oblong with either one long flagellum, one long and one short flagellum, or 2 long flagella. The flagella may occur together or at opposite ends of the cell. Upon emergence, the swarmcells are 6-8 µm in length and can develop to around 12 µm in older cells.¹

Ceratiomyxa plasmodia is diploid and can often be found emerging from rotting wood and spreading into a thin layer. The plasmodium then fruits to form sporangia: erect pillar or wall-like structures. The sporangia mature in about six hours. Many small stalks emerge from the sporangia to form protospores. Meiosis then begins in the protospores. Synapsis of the chromosomes in prophase is achieved at this stage.

After the stalks have fully elongated, a thin transparent wall is secreted around the protospores to become spores. Spores take approximately 24 hours to mature. The first meiotic division is then completed in the spores followed by a second meiotic division which leaves the spore with 4 nuclei.

After dispersal, these four-nucleate spores germinate and naked protoplasts emerge. The protoplasts take in water upon emergence and enlarge to about 3 times the size of the spore from which they emerged. The protoplasts then form short thread-like filaments that later retract back into round globules. In some cases, the protoplast skips the thread phase and remains isodiametric. The nuclei then migrate to four points of a tetrahedron and the protoplast cleaves into a tetrad of lobes. Soon after, the tetrad undergoes mitosis and splits again into an octette of haploid cells.

Each cell releases a swarmcell with either one long flagellum, 2 unequal flagella, or 2 long flagella. The flagella are apical and whiplash type. The swarmcells may then lose their flagella and become asexual myxamoebae or undergo syngamy in pairs to produce a diploid zygote.^1,2

Genus Ceratiomyxa currently includes four species:

• Ceratiomyxa fruticulosa Micheli, 1729
• Ceratiomyxa sphaerosperma Boedijn, 1927
• Ceratiomyxa morchella Weldon, 1954
• Ceratiomyxa hemisphaerica Olive and Stoianovitch, 1979

¹ Gilbert, H., C. 1935: Critical events in the life history of Ceratiomyxa. Am. J. Bot. 22: 52-74

² Olive, L. S. and Stoianovitch, C. 1979: Observations on the Mycetozoan genus Ceratiomyxa: Description of a new species. Mycologia. 71: 546-555. doi: 10.2307/3759064

³ Rojas, C., Schnittler, M., Biffi, D., and Stephenson, S. L. 2008: Microhabitat and niche separation in species of Ceratiomyxa. Mycol. Soc. Am. 100: 843-850