Spinal interneuron

A spinal interneuron, found in the spinal cord, relays signals between (afferent) sensory neurons, and (efferent) motor neurons. Different classes of spinal interneurons are involved in the process of sensory-motor integration.[1] Most interneurons are found in the grey column, a region of grey matter in the spinal cord.

Spinal interneuron
Spinal interneuron integrates sensory-motor input
Anatomical terminology

Structure

The grey column of the spinal cord appears to have groups of small neurons, often referred to as spinal interneurons, that are neither primary sensory cells nor motor neurons.[2] The versatile properties of these spinal interneurons cover a wide range of activities. Their functions include the processing of sensory input, the modulation of motor neuron activity, the coordination of activity at different spinal levels, and the relay of sensory or proprioceptive data to the brain. There has been extensive research on the identification and characterization of the spinal cord interneurons based on factors such as location, size, structure, connectivity, and function.[2] Generally, it is difficult to characterize every aspect of the neuronal anatomy of a vertebrate's spinal cord. This difficulty is due not only to its structural complexity but also to the morphology and the connectivity of neurons. For instance, in the spinal cord of a 19-day-old rat embryo, at least 17 different subclasses of interneurons with ipsilateral axon projections were found. In addition, 18 types of commissural interneurons have been identified on the basis of morphology and location.[3][4]

Location

In particular, the cell bodies of the spinal interneurons are found in the grey matter of the spinal cord, which also contains the motor neurons. In 1952, the grey matter of the cat's spinal cord was investigated, and it was shown to have ten distinct zones referred to as Rexed laminae. Eventually, the lamination pattern was also observed in several species including humans. Rexed laminae VII and VIII are locations where most of the interneurons are found.[5]

Rexed laminae

Development

In the mouse's dorsal alar plate, six progenitor domains give rise to dI1-dI6 neurons and two classes of dorsal interneurons.[6] In addition, in the ventral half of the neural tube, four classes of (CPG) interneurons known as V0, V1, V2, and V3 neurons are generated.[6] V0 neurons are commissural neurons that extend their axons rostrally for 2-4 spinal cord regions in the embryonic spinal cord.[6] V3 neurons are excitatory commissural interneurons that extend caudally projecting primary axons.[6] The V1 neurons are inhibitory interneurons with axons that project ipsilaterally and rostrally.[6] V2 neurons, which include a population of glutamatergic V2a neurons and inhibitory V2b neurons, project ipsilaterally and caudally across multiple spinal cord regions.[6] The class V1 neurons give rise to two local circuit inhibitory neurons known as Renshaw cells and Ia inhibitory interneurons.[6]

CPG interneuronsTypeAxon projection in embryonic cord
V0CommissuralRostrally
V1Inhibitory (Renshaw cells and Ia interneurons)Rostrally and ipsilaterally
V2Glutamatergic V2a and Inhibitory V2bIpsilaterally and caudally
V3Excitatory CommissuralCaudally

Function

The integration of the sensory feedback signals and central motor commands at several levels of the central nervous system plays a critical role in controlling movement.[7] Research on cat's spinal cord has shown that at the spinal cord level sensory afferents and descending motor pathways converge onto common spinal interneurons.[7] Human studies since the 1970s have documented how this integration of motor commands and sensory feedback signals is used to control muscle activity during movement.[7] During locomotion, the sum of convergent inputs from the central pattern generator (CPG), sensory feedback, descending commands and other intrinsic properties turned on by different neuromodulators give rise to the activity of the interneurons.[8] Further, this interneuronal activity was either recorded directly or inferred from the modulation of response in their postsynaptic targets, most often motoneurons.[8] The most efficient way to gate sensory signals in reflex pathways is to control the firing level of interneurons. For example, during locomotion, the interneuronal activity is modulated via excitation or inhibition depending on the reflex pathways.[8] Thus, different patterns of interneuronal activity will determine which pathways are open, blocked, or modulated.[8]

Neurotransmitter

The sensory information that is transmitted to the spinal cord is modulated by a complex network of excitatory and inhibitory interneurons. Different neurotransmitters are released from different interneurons, but the two most common neurotransmitters are GABA, the primary inhibitory neurotransmitter and glutamate, the primary excitatory neurotransmitter.[9][10] Acetylcholine is a neurotransmitter that often activates interneurons by binding to a receptor on the membrane.[11]

Renshaw cells

Renshaw cells are among the first identified interneurons.[12] This type of interneuron projects onto α-motoneurons, where it establishes inhibition by expressing its inhibitory neurotransmitter glycine.[12][13] However, some reports have indicated that Renshaw cells synthesize calcium-binding proteins calbindin-D28k and parvalbumin. Further, during spinal reflex, Renshaw cells control the activity of the spinal motoneurons. They are excited by the axon collaterals of the motor neurons. In addition, Renshaw cells make inhibitory connections to several groups of motor neurons, Ia inhibitory interneurons as well as the same motor neuron that excited them previously.[13] Furthermore, the connection to the motor neurons establishes a negative feedback system that may regulate the firing rate of the motor neurons.[13] Moreover, the connections to the Ia inhibitory interneurons may modulate the strength of the reciprocal inhibition to the antagonist motor neuron.[13]

Ia inhibitory interneuron

Joints are controlled by two opposing sets of muscles called extensors and flexors that must work in synchrony to allow proper and desired movement.[14] When a muscle spindle is stretched and the stretch reflex is activated, the opposing muscle group must be inhibited to prevent from working against the agonist muscle.[12][14] The spinal interneuron called Ia inhibitory interneuron is responsible for this inhibition of the antagonist muscle.[14] The Ia afferent of the muscle spindle enters the spinal cord, and one branch synapses on to the alpha motor neuron that causes the agonist muscle to contract.[14] Thus, it results in creating the behavioral reflex.

At the same time, the other branch of the Ia afferent synapses on to the Ia inhibitory interneuron, which in turn synapses the alpha motor neuron of the antagonist muscle.[14] Since Ia interneuron is inhibitory, it prevents the opposing alpha motor neuron from firing. Thus, it prevents the antagonist muscle from contracting.[14] Without having this system of reciprocal inhibition, both groups of muscles may contract at the same time and work against each other. This results in spending a greater amount of energy as well.

In addition, the reciprocal inhibition is important for mechanism underlying voluntary movement.[14] When the antagonist muscle relaxes during movement, this increases efficiency and speed. This prevents moving muscles from working against the contraction force of antagonist muscles.[14] Thus, during voluntary movement, the Ia inhibitory interneurons are used to coordinate muscle contraction.

Further, the Ia inhibitory interneurons allow the higher centers to coordinate commands sent to the two muscles working opposite of each other at a single joint via a single command.[14] The interneuron receives the input command from the corticospinal descending axons in such a way that the descending signal, which activates the contraction of one muscle, causes relaxation of the other muscles.[12][13][14][15]

Ib inhibitory interneuron

The autogenic inhibition reflex is a spinal reflex phenomenon that involves the Golgi tendon organ.[14] When tension is applied to a muscle, group Ib fibers that innervate the Golgi tendon organ are activated. These afferent fibers project onto the spinal cord and synapse with the spinal interneurons called Ib inhibitory interneurons.[14] This spinal interneuron makes an inhibitory synapse onto the alpha motor neuron that innervates the same muscle that caused the Ib afferent to fire. As a result of this reflex, activation of the Ib afferent causes the alpha motor neuron to become inhibited. Thus, the contraction of the muscle stops.[14] This is an example of a disynaptic reflex, in which the circuitry contains a spinal interneuron between the sensory afferent and the motor neuron.[13][14]

The activities of the extensor and flexor muscles must be coordinated in the autogenic inhibition reflex. The Ib afferent branches in the spinal cord. One branch synapses the Ib inhibitory interneuron. The other branch synapses onto an excitatory interneuron. This excitatory interneuron innervates the alpha motor neuron that controls the antagonist muscle. When the agonist muscle is inhibited from contracting, the antagonist muscle contracts.[14]

Excitatory interneurons

An important reflex initiated by cutaneous receptors and pain receptors is the flexor reflex.[14] This reflex mechanism allows for quick withdrawal of the body parts, in this case a limb, from the harmful stimulus. The signal travels to the spinal cord and a response is initiated even before it travels up to the brain centers for a conscious decision to be made.[14] The reflex circuit involves the activation of the Group III afferents of pain receptors due to a stimulus affecting a limb, e.g. a foot. These afferents enter the spinal cord and travel up to the lumbar region, where they synapse an excitatory interneuron.[14] This interneuron excites the alpha motor neuron that causes contraction of the thigh flexor muscle.

Also, Group III afferent travels up to L2 vertebra, where they branch onto another excitatory interneuron. This interneuron excites the alpha motor neurons, which then excite the hip flexor muscle.[14] This synchronized communication allows for the removal of the whole leg from the painful stimulus. This is an example of the spinal cord circuitry coordinating movement at several joints simultaneously. In addition, during flexor reflex, when the knee joints and hip joints are flexed, the antagonist extensor muscles must be inhibited.[14] This inhibitory effect is achieved when Group III afferents synapse inhibitory interneurons that in turn synapse the alpha motor neurons innervating the antagonists muscle.[14]

The flexor reflex not only coordinates the activity of the leg being removed but also the activity of the other leg. When one leg is removed, the weight of the body needs to be distributed to the opposite leg to maintain the body’s balance. Thus, the flexor reflex incorporates a crossed extension reflex. A branch of the Group III afferent synapse an excitatory interneuron, which extends its axon across the midline into the contralateral spinal cord. At that location, the interneuron excites the alpha motor neurons that innervate the extensor muscles of the opposite leg. This allows for balance and body posture to be maintained.[14]

References
  1. Rose PK, Scott SH (December 2003). "Sensory-motor control: a long-awaited behavioral correlate of presynaptic inhibition". Nature Neuroscience. 6 (12): 1243–5. doi:10.1038/nn1203-1243. PMID 14634653.
  2. Lowrie MB, Lawson SJ (August 2000). "Cell death of spinal interneurones". Progress in Neurobiology. 61 (6): 543–55. doi:10.1016/S0301-0082(99)00065-9. PMID 10775796.
  3. Silos-Santiago I, Snider WD (November 1992). "Development of commissural neurons in the embryonic rat spinal cord". The Journal of Comparative Neurology. 325 (4): 514–26. doi:10.1002/cne.903250405. PMID 1469113.
  4. Silos-Santiago I, Snider WD (April 1994). "Development of interneurons with ipsilateral projections in embryonic rat spinal cord". The Journal of Comparative Neurology. 342 (2): 221–31. doi:10.1002/cne.903420206. PMID 8201033.
  5. Goshgarian, HG (2003). Neuroanatomic Organization of the Spinal Gray and White Matter. New York: Demos Medical Publishing.
  6. Goulding M (July 2009). "Circuits controlling vertebrate locomotion: moving in a new direction". Nature Reviews. Neuroscience. 10 (7): 507–18. doi:10.1038/nrn2608. PMC 2847453. PMID 19543221.
  7. Nielsen JB (May 2004). "Sensorimotor integration at spinal level as a basis for muscle coordination during voluntary movement in humans". Journal of Applied Physiology. 96 (5): 1961–7. doi:10.1152/japplphysiol.01073.2003. PMID 15075316.
  8. Rossignol S, Dubuc R, Gossard JP (January 2006). "Dynamic sensorimotor interactions in locomotion". Physiological Reviews. 86 (1): 89–154. doi:10.1152/physrev.00028.2005. PMID 16371596.
  9. Manent, Jean-Bernard; Represa, Alfonso (1 June 2007). "Neurotransmitters and Brain Maturation: Early Paracrine Actions of GABA and Glutamate Modulate Neuronal Migration". The Neuroscientist. 13 (3): 268–279. doi:10.1177/1073858406298918. PMID 17519369.
  10. Bardoni R, Takazawa T, Tong CK, Choudhury P, Scherrer G, Macdermott AB (March 2013). "Pre- and postsynaptic inhibitory control in the spinal cord dorsal horn". Annals of the New York Academy of Sciences. 1279: 90–6. doi:10.1111/nyas.12056. PMC 7359868. PMID 23531006.
  11. Alkondon, M; Peirera, EF; Eisenberg, HM; Albuquerque, EX (1 January 2000). "Nicotinic receptor activation in human cerebral cortical interneurons: a mechanism for inhibition and disinhibition of neuronal networks". Journal of Neuroscience. 20 (1): 66–75. doi:10.1523/jneurosci.20-01-00066.2000. PMC 6774099. PMID 10627582.
  12. Nishimaru H, Kakizaki M (October 2009). "The role of inhibitory neurotransmission in locomotor circuits of the developing mammalian spinal cord". Acta Physiologica. 197 (2): 83–97. doi:10.1111/j.1748-1716.2009.02020.x. PMID 19673737.
  13. Hultborn H (2006). "Spinal reflexes, mechanisms and concepts: from Eccles to Lundberg and beyond". Progress in Neurobiology. 78 (3–5): 215–32. doi:10.1016/j.pneurobio.2006.04.001. PMID 16716488.
  14. Knierim, James (2013). Spinal Reflexes and Descending Motor Pathways. Houston: UTHealth.
  15. Nógrádi A, Vrbová G (2013). Anatomy and Physiology of the Spinal Cord. Landes Bioscience.
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