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Registry of Toxic Effects of Chemical Substances (RTECS)

Aniline

RTECS #

BW6650000

CAS #

62-53-3

Updated

September 201

Molecular Weight

93.14

Molecular Formula

C6H7N

Synonyms

Aminobenzene
Aminophen
Anilin (Czech)
Anilina (Italian, Polish)
Aniline and homologues (ACGIH:OSHA)
Aniline oil
Anyvim
Benzenamine
Benzene, amino
Benzidam
Blue Oil
C.I. 76000
C.I. Oxidation Base 1
Cyanol
Huile d'aniline (French)
Krystallin
Kyanol
NCI-C03736
Phenylamine
RCRA waste number U012

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 20 mg/24H moderate85JCAE -,456,1986
skin /rabbit 20 mg/24H moderate85JCAE -,456,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
body fluid assaySalmonella typhimurium/rat300 mg/kgMUREAV 79,173,1980
Cytogenetic Analysisovary/hamster444 mg/LEMMUEG 27,67,1996
Cytogenetic Analysisfibroblast/hamster1000000 µg/L (+enzymatic activation step)HPV371 -,-,2006
DNA adductoral/rat1397 mg/kg/30D-continuousTOXID9 -,345,2009
DNA Damageother cell types/rat1397.1 mg/kg/30D-continuousTOXID9 -,15,2008
DNA Damageoral/rat1397 mg/kg/30D- intermittentTXAPA9 233,247,2008
DNA Damageintraperitoneal/mouse300 mg/kgATSUDG 5,355,1982
DNA Damagelymphocyte/mouse21500 µmol/LMUREAV 203,155,1988
DNA Damageoral/mouse1 gm/kgMUREAV 440,1,1999
DNA Damageintraperitoneal/rat105 mg/kgCNREA8 42,2277,1982
DNA repair/Escherichia coli39658 µg/well/16HCBINA8 15,219,1976
micronucleus testintraperitoneal/mouse50 mg/kgJPMSAE 80,761,1991
micronucleus testunreported route/mouse23 mg/kgJAPTO* 25,514,2005
micronucleus testlung/hamster2500 µmol/L/6H (-enzymatic activation step)MUTAEX 26,709,2011
micronucleus testlung/hamster5000 µmol/L/6H (+enzymatic activation step)MUTAEX 26,709,2011
micronucleus testlung/hamster2500 µmol/L/48H (-enzymatic activation step)MUTAEX 26,709,2011
morphological transformfibroblast/mouse0.01 mg/L/21D (-enzymatic activation step)EMMUEG 35,300,2000
morphological transformfibroblast/mouse800 µg/LJJIND8 67,1303,1981
mutation in mammalian somatic cellslymphocyte/mouse2500 µmol/LMUTAEX 3,193,1988
mutation in mammalian somatic cellslung/hamster500 µg/LBLFSBY 39,359,1986
mutation in microorganisms/Salmonella typhimurium344 nmol/plate/48H (+enzymatic activation step)MUREAV 541,123,2003
mutation in microorganisms/Salmonella typhimurium1 µg/plateMUREAV 577S,1,2005
mutation in microorganismslymphocyte/mouse500 µmol/L (+enzymatic activation step)MUTAEX 3,193,1988
mutation in microorganisms/Salmonella typhimurium100 µg/plate (-enzymatic activation step)PJABDW 53,34,1977
sister chromatid exchangelymphocyte/human300 µmol/LMUREAV 395,159,1997
sister chromatid exchangeliver/rat200 µmol/LTOLED5 16,235,1983
sister chromatid exchangeovary/hamster50 mg/LEMMUEG 10(Suppl 10),1,1987
sister chromatid exchangeintraperitoneal/mouse210 mg/kgCNREA8 42,2277,1982
specific locus testlymphocyte/mouse500 mg/LEMMUEG 17,196,1991

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/mouse 4480 mg/kg (6-13D pregnant)Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)TCMUD8 7,29,1987

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/rat lowest published toxic dose: 11 gm/kg/29W- continuousTumorigenic: Neoplastic by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors		APMIAL 26,472,1949
oral/rat toxic dose: 72800 mg/kg/2Y- continuousTumorigenic: Neoplastic by RTECS criteria

Blood: Tumors		FCTOD7 25,619,1987

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Rat, liver Inhibitor Concentration (50 percent kill): 1226 mg/L/20HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)TIVIEQ 5,543,1991
In Vitro/Rat, liver Inhibitor Concentration (50 percent kill): 1430 mg/L/20HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 5,543,1991
In Vitro/Rat, liver Inhibitor Concentration (50 percent kill): 1226 mg/L/20HIn Vitro Toxicity Studies: Cell protein synthesisTIVIEQ 5,543,1991
In Vitro/Rat, liver Inhibitor Concentration (50 percent kill): 920 mg/L/20HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 5,543,1991
In Vitro/Rat, liver tumor Inhibitor Concentration (50 percent kill): 3072 mg/L/20HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)TIVIEQ 5,543,1991
In Vitro/Rat, liver tumor Inhibitor Concentration (50 percent kill): 2700 mg/L/20HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 5,543,1991
In Vitro/Rat, liver tumor Inhibitor Concentration (50 percent kill): 2793 mg/L/20HIn Vitro Toxicity Studies: Cell protein synthesisTIVIEQ 5,543,1991
In Vitro/Rat, liver tumor Inhibitor Concentration (50 percent kill): 4003 mg/L/20HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 5,543,1991
inhalation/cat Lowest published lethal concentration: 180 ppm/8HXPHBAO 271,4,1941
inhalation/Dog lowest published toxic concentration: 23.6 mg/m3Blood: Methemoglobinemia- CarboxhemoglobinemiaTXCYAC 214,140,2005
inhalation/mammal (species unspecified) lethal concentration (50 percent kill): 2500 mg/m3GTPZAB 32(10),25,1988
inhalation/mouse lethal concentration (50 percent kill): 175 ppm/7HNTIS** PB214-270
inhalation/rat Lowest published lethal concentration: 250 ppm/4HJIHTAB 31,343,1949
inhalation/rat lethal concentration (50 percent kill): 250 ppm/1HENTOX* -,136,2005
inhalation/rat lowest published toxic concentration: 0.02 gm/m3/4HBehavioral: Somnolence (general depressed activity)

Behavioral: Change in motor activity (specific assay)

Behavioral: Muscle weakness		TPKVAL 12,54,1971
inhalation/rat lowest published toxic concentration: 0.09 gm/m3/4HBehavioral: Alteration of classical conditioning

Vascular: Other changes

Gastrointestinal: Ulceration or bleeding from stomach		TPKVAL 12,54,1971
inhalation/rat lowest published toxic concentration: 0.006 gm/m3/4HTPKVAL 12,54,1971
intraperitoneal/guinea pig lethal dose (50 percent kill): 100 mg/kgTSCAT* OTS 206512
intraperitoneal/mouse lethal dose (50 percent kill): 368 mg/kgHPV300 -,-,2001
intraperitoneal/rat lethal dose (50 percent kill): 420 mg/kgAGGHAR 15,447,1957
intravenous/Dog lowest published lethal dose: 200 mg/kgNTIS** PB214-270
intravenous/rabbit lethal dose (50 percent kill): 64 mg/kgNTIS** PB214-270
oral/cat lowest published lethal dose: 100 mg/kgHBAMAK 4,1301,1935
oral/child lowest published toxic dose: 3125 mg/kgLung, Thorax, or Respiration: CyanosisJTCTDW 26,357,1988
oral/Dog lethal dose (50 percent kill): 195 mg/kgNTIS** PB214-270
oral/guinea pig lethal dose (50 percent kill): 400 mg/kgTSCAT* OTS 206512
oral/mammal (species unspecified) lethal dose (50 percent kill): 500 mg/kgGTPZAB 32(10),25,1988
oral/mouse lethal dose (50 percent kill): 464 mg/kgENTOX* -,136,2005
oral/quail lethal dose (50 percent kill): 750 mg/kgAECTCV 12,355,1983
oral/rabbit lowest published lethal dose: 500 mg/kgHBAMAK 4,1301,1935
oral/rat lethal dose (50 percent kill): 250 mg/kgJPETAB 90,260,1947
oral/rat lowest published toxic dose: 1118 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaTPKVAL 14,118,1975
oral/rat lowest published toxic dose: 1000 mg/kgPeripheral Nerve and Sensation: Flaccid paralysis without anesthesia (usually neuromuscular blockage)

Spinal Cord: Demyelination

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		TOPADD 14,19,2001
oral/rat lowest published toxic dose: 1000 mg/kgSpinal Cord: Other degenerative changes

Biochemical: Metabolism (intermediary): Effect on cyclic nucleotides		TOXID9 12,470,2008
oral/wild bird lethal dose (50 percent kill): 562 mg/kgAECTCV 12,355,1983
skin/cat lethal dose (50 percent kill): 254 mg/kgGTPZAB 13(5),29,1969
skin/Dog lowest published lethal dose: 1540 mg/kgNTIS** PB214-270
skin/guinea pig lethal dose (50 percent kill): 1290 mg/kgTXAPA9 7,559,1965
skin/rabbit lethal dose (50 percent kill): 820 µL/kgTXAPA9 7,559,1965
skin/rat lethal dose (50 percent kill): 1400 mg/kgENTOX* -,136,2005
subcutaneous/cat lowest published lethal dose: 100 mg/kgHBAMAK 4,1301,1935
subcutaneous/mouse lethal dose (50 percent kill): 200 mg/kgARZNAD 8,107,1958
subcutaneous/rabbit lowest published lethal dose: 1 gm/kgLung, Thorax, or Respiration: Other changesMELAAD 28,112,1937
unreported route/man lowest published lethal dose: 350 mg/kgJIDHAN 13,87,1931
unreported route/man lowest published lethal dose: 150 mg/kg85DCAI 2,73,1970

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/rat lowest published toxic concentration: 3 mg/m3/22W- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Blood: Other changes		GISAAA 37(5),31,1972
inhalation/rat lowest published toxic concentration: 87 ppm/6H/2W- intermittentLiver: Changes in liver weight

Endocrine: Changes in spleen weight

Blood: Changes in erythrocyte (RBC) count		TOXID9 2,164,1982
inhalation/rat lowest published toxic concentration: 300 µg/m3/24H/80D- continuousBehavioral: Muscle contraction or spasticityGISAAA 35(4),44,1970
inhalation/rat lowest published toxic concentration: 5 mg/m3/24H/21D- continuousBlood: Methemoglobinemia- CarboxhemoglobinemiaGISAAA 37(12),53,1972
inhalation/rat lowest published toxic concentration: 30 ppm/14D- continuousBlood: Other hemolysis with or without anemia

Blood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in spleen		HPV371 -,,2006
intraperitoneal/mouse lowest published toxic dose: 894 mg/kg/6W- intermittentImmunological Including Allergic: Autoimmune (multiple organ involvement)TOXID9 44,187,2005
oral/rat lowest published toxic dose: 1397.1 mg/kg/30D- continuousBlood: Changes in spleen

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes

Biochemical: Metabolism (intermediary): Other proteins		TOXID9 -,22,2008
oral/rat lowest published toxic dose: 651.98 mg/kg/7D- intermittentBlood: Changes in spleenTXAPA9 230,227,2008
oral/rat lowest published toxic dose: 1397 mg/kg/30D- continuousBlood: Changes in spleenTOXID9 -,345,2009
oral/rat lowest published toxic dose: 2200 mg/kg/20D- continuousEndocrine: Changes in spleen weight

Blood: Changes in bone marrow not included in above

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field		HPV371 -,,2006
oral/rat lowest published toxic dose: 913 mg/kg/2W- intermittentEndocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Changes in: Fe

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases		FAATDF 25,224,1995
oral/rat lowest published toxic dose: 550 mg/kg/5D- continuousBlood: Changes in spleen

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field		FAATDF 3,285,1983
oral/rat lowest published toxic dose: 210 mg/kg/12W- continuousBlood: Pigmented or nucleated red blood cells

Blood: Methemoglobinemia- Carboxhemoglobinemia

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other esterases		GISAAA 24(7),44,1959
subcutaneous/rat lowest published toxic dose: 900 mg/kg/6D- intermittentLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Dehydrogenases		BCTKAG 13,99,1980

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 2 ppm (skin)DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMSUDL 7,99,1987
International Agency for Research on Cancer (IARC)Cancer Review:Animal Inadequate EvidenceIMEMDT 4,27,1974
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMSUDL 7,99,1987
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMSUDL 7,99,1987
TOXICOLOGY REVIEWAJMEAZ 38,409,1965
TOXICOLOGY REVIEWTOLED5 140-141,229,2003
TOXICOLOGY REVIEWTOLED5 140-141,183,2003
TOXICOLOGY REVIEWTXAPA9 197,144,2004
TOXICOLOGY REVIEWDIMON* 39,678,1993
TOXICOLOGY REVIEWENTOX* -,136,2005
TOXICOLOGY REVIEWENTOX* -,104,2005
TOXICOLOGY REVIEWMUREAV 627,78,2007
TOXICOLOGY REVIEWHUTOX* -,649,1996
TOXICOLOGY REVIEWBLREV* 3,71,1989
TOXICOLOGY REVIEWMUTAEX 23,271,2008
TOXICOLOGY REVIEWTXAPA9 243,180,2010
TOXICOLOGY REVIEWNTAPM* -,629,1995
TOXICOLOGY REVIEWMUREAV 705,184,2010
TOXICOLOGY REVIEWMUREAV 752,99,2013

Standards and Regulations

OrganizationStandardReference
Mine Safety and Health Administration (MSHA) STANDARD-airtime-weighted average 5 ppm (19 mg/m3)DTLVS* 3,13,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-ARAB Republic of Egypttime-weighted average 2 ppm (10 mg/m3), Skin, JAN1993
Occupational Exposure Limit-AUSTRALIAtime-weighted average 2 ppm (7.6 mg/m3), JUL2008
Occupational Exposure Limit-AUSTRIAMAK-TMW 2 ppm (8 mg/m3);KZW 10 ppm (40 mg/m3), skin, sen, 2007
Occupational Exposure Limit-BELGIUMtime-weighted average 2 ppm (7.7 mg/m3), Skin, MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 1 ppm (4 mg/m3), skin, carc, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 2 ppm (7.7 mg/m3), short term exposure limit 4 ppm (15 mg/m3), skin, NOV2011
Occupational Exposure Limit-FRANCEVME 2 ppm (10 mg/m3), Skin, C3 Carcinogen, FEB2006
Occupational Exposure Limit-GERMANYMAK 2 ppm (7.7 mg/m3), skin, 2011
Occupational Exposure Limit-HUNGARYtime-weighted average 8 mg/m3, short term exposure limit 32 mg/m3, Skin, SEP2000
Occupational Exposure Limit-ICELANDtime-weighted average 1 ppm (4 mg/m3), skin, NOV2011
Occupational Exposure Limit-INDIAtime-weighted average 0.2 mg/m3, Skin, JAN1993
Occupational Exposure Limit-JAPANOccupational Exposure Limit 1 ppm (3.8 mg/m3), skin, s1 sen(pr), MAY2012
Occupational Exposure Limit-KOREAtime-weighted average 2 ppm (10 mg/m3), skin, 2006
Occupational Exposure Limit-MEXICOtime-weighted average 2 ppm (10 mg/m3);short term exposure limit 5 ppm (20 mg/m3) (skin), 2004
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 1 ppm (4 mg/m3), skin, JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 1 ppm (4 mg/m3), JAN1999
Occupational Exposure Limit-PERUtime-weighted average 2 ppm (7.6 mg/m3), JUL2005
Occupational Exposure Limit-POLANDMAC(time-weighted average) 5 mg/m3, MAC(short term exposure limit) 20 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAtime-weighted average 0.1 mg/m3, short term exposure limit 0.3 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWEDENtime-weighted average 1 ppm (4 mg/m3);short term exposure limit 2 ppm (8 mg/m3), Skin, Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 2 ppm (8 mg/m3), KZG-week 4 ppm (16 mg/m3), carc 3, skin, JAN2011
Occupational Exposure Limit-THE NETHERLANDSMAC-TGG 1 mg/m3, Skin, 2003
Occupational Exposure Limit-THE PHILIPPINEStime-weighted average 5 ppm (19 mg/m3), Skin, JAN1993
Occupational Exposure Limit-TURKEYtime-weighted average 5 ppm (19 mg/m3), Skin, JAN1993
Occupational Exposure Limit-UNITED KINGDOMtime-weighted average 1 ppm (4 mg/m3), skin, OCT2007
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 5 ppm (19 mg/m3) (skin)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 5 ppm (19 mg/m3) (skin)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 5 ppm (19 mg/m3) (skin)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 5 ppm (19 mg/m3) (skin)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO ANILINE AND HOMOLOGS-airCarcinogen lowest feasible conc.NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code 06580; Number of Industries 21; Total Number of Facilities 748; Number of Occupations 39; Total Number of Employees Exposed 41988; Total Number of Female Employees Exposed 14949
National Occupational Hazard Survey 1974Hazard Code 06580; Number of Industries 25; Total Number of Facilities 1868; Number of Occupations 46; Total Number of Employees Exposed 21859

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test; S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Negative: SHE-clonal assay; E coli polA with S9
EPA GENETOX PROGRAM 1988, Positive/dose response: Cell transform.-BALB/c-3T3
EPA GENETOX PROGRAM 1988, Positive: E coli polA without S9
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Amines, aromatic, 2002
On EPA IRIS database

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