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Registry of Toxic Effects of Chemical Substances (RTECS)

Methane, tribromo-

RTECS #

PB5600000

CAS #

75-25-2

Updated

September 201

Molecular Weight

252.75

Molecular Formula

CHBr3

Synonyms

Bromoform
Bromoform (ACGIH:OSHA)
Bromoforme (French)
Bromoformio (Italian)
Methenyl tribromide
Methyl tribromide
NCI-C55130
RCRA waste number U225
Tribrommethaan (Dutch)
Tribrommethan (German)
Tribromometan (Italian)
Tribromomethane

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisintraperitoneal/rat25300 µg/kgMUREAV 242,111,1990
Cytogenetic Analysisoral/rat1265 mg/kg/5D- intermittentMUREAV 242,111,1990
Cytogenetic Analysisovary/hamster110 µg/LENMUDM 7,1,1985
DNA Damagelung/human100 µmol/L/3HMUREAV 538,41,2003
DNA Damageother cell types/human1 mmol/L/4HMUREAV 741,89,2012
micronucleus testintratracheal/mouse800 mg/kg/48H- intermittentNTPTR* NTP-TR-350,1989
micronucleus testmultiple/non-mammalian species5 ppmMUREAV 292,83,1993
morphological transformfibroblast/mouse0.05 mg/L/21D (-enzymatic activation step)EMMUEG 37,231,2001
mutation in mammalian somatic cellslymphocyte/mouse200 mg/LNTPTR* NTP-TR-350,1989
mutation in microorganisms/Salmonella typhimurium1000 mg/L/2H (-enzymatic activation step)EMMUEG 44,329,2004
mutation in microorganisms/Salmonella typhimurium666 µg/plate (+enzymatic activation step)ENMUDM 5(Suppl 1),3,1983
mutation in microorganisms/Salmonella typhimurium100 µg/plate (-enzymatic activation step)NTPTR* NTP-TR-350,1989
sex chromosome loss and nondisjunctionoral/Drosophila melanogaster3000 ppmENMUDM 7,677,1985
sister chromatid exchangeovary/hamster290 µg/LENMUDM 7,1,1985
sister chromatid exchangelymphocyte/human80 µmol/LENVRAL 32,72,1983
sister chromatid exchangeoral/mouse100 mg/kg/4D- intermittentENVRAL 32,72,1983
sister chromatid exchangeintratracheal/mouse800 gm/LNTPTR* NTP-TR-350,1989
sister chromatid exchangeleukocyte/rat20 µmol/LMUREAV 300,241,1993
specific locus testoral/Drosophila melanogaster3000 ppmNTPTR* NTP-TR-350,1989

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/mouse 5.6 gm/kg (7D prior to copulation/21D pregnant)Reproductive: Maternal effects: PostpartumNTIS** #PB89169254
oral/mouse 47 gm/kg (15W male/15W pre-4D after birth)Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive)

Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)		NTIS** #PB89169254
oral/mouse 200 mg/kg (multigenerations)Reproductive: Specific developmental abnormalities: Hepatobiliary system

Reproductive: Specific developmental abnormalities: Urogenital system		NTIS** #PB89169254
oral/mouse 200 mg/kg (multigenerations)Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)NTIS** #PB89169254

Tumorigenic Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse lowest published toxic dose: 1100 mg/kg/8W- intermittentTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors		CNREA8 37,2717,1977
oral/rat lowest published toxic dose: 103000 mg/kg/103W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors		NTIS** PB90-110149/AS
oral/rat lowest published toxic dose: 103000 mg/kg/103W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Tumors		NTIS** PB90-110149/AS
oral/rat lowest published toxic dose: 10400 mg/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Tumors		NTPTR* NTP-TR-350,1989

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/mammal (species unspecified) lethal concentration (50 percent kill): 12100 mg/m3GTPZAB 18(4),55,1974
inhalation/mouse lethal concentration (50 percent kill): 12100 mg/m3/2HLung, Thorax, or Respiration: Other changesVCVGH* -,581,1990
inhalation/rat lowest published toxic concentration: 3900 mg/m3/2HBrain and Coverings: Changes in surface EEGVCVGH* -,581,1990
inhalation/rat Lowest published lethal concentration: 45 gm/m3/4H85GMAT -,28,1982
intraperitoneal/mouse lethal dose (50 percent kill): 1274 mg/kgVCVGH* -,572,1990
intraperitoneal/rat lethal dose (50 percent kill): 414 mg/kgEye: Lacrimation

Behavioral: Somnolence (general depressed activity)

Behavioral: Antipsychotic		TOLED5 15,251,1983
oral/human lowest published lethal dose: 143 mg/kg34ZIAG -,141,1969
oral/mouse lethal dose (50 percent kill): 1072 mg/kgLung, Thorax, or Respiration: DyspneaNTPTR* NTP-TR-350,1989
oral/rat lethal dose (50 percent kill): 933 mg/kgLung, Thorax, or Respiration: DyspneaNTPTR* NTP-TR-350,1989
subcutaneous/mouse lethal dose (50 percent kill): 1820 mg/kgTXAPA9 4,354,1962
subcutaneous/rabbit lowest published lethal dose: 410 mg/kgAEXPBL 28,201,1891

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 3500 mg/kg/14D- intermittentLiver: Changes in liver weight

Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		EVHPAZ 46,117,1982
oral/mouse lowest published toxic dose: 6500 mg/kg/13W- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldNTPTR* NTP-TR-350,1989
oral/mouse lowest published toxic dose: 296 mg/kg/14D- intermittentKidney, Ureter, and Bladder: Changes primarily in glomeruli

Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)		VCVGH* -,581,1990
oral/mouse lowest published toxic dose: 21000 mg/kg/15W- intermittentBehavioral: Fluid intakeNTIS** #PB89169254
oral/rat lowest published toxic dose: 6230 mg/kg/4W- continuousLiver: Other changes

Liver: Changes in liver weight

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)		JTSCDR 17,119,1992
oral/rat lowest published toxic dose: 13 gm/kg/13W- intermittentLiver: Other changesNTPTR* NTP-TR-350,1989
oral/rat lowest published toxic dose: 5600 mg/kg/14D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldNTPTR* NTP-TR-350,1989
oral/rat lowest published toxic dose: 5840 mg/kg/1Y- continuousKidney, Ureter, and Bladder: Other changes in urine compositionTOXID9 14,281,1994

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 0.5 ppmDTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMEMDT 52,213,1991
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMEMDT 71,1309,1999
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 52,213,1991
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 71,1309,1999
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMEMDT 71,1309,1999
TOXICOLOGY REVIEWMUREAV 564,179,2004
TOXICOLOGY REVIEWMUREAV 567,347,2004
TOXICOLOGY REVIEWTXCYAC 198,239,2004
TOXICOLOGY REVIEWENTOX* -,345,2005
TOXICOLOGY REVIEWENTOX* -,546,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWMUREAV 636,242,2007
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWMUREAV 608,157,2006

Standards and Regulations

OrganizationStandardReference
Mine Safety and Health Administration (MSHA) STANDARD-airtime-weighted average 0.5 ppm (5 mg/m3) (skin)DTLVS* 3,28,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 0.5 ppm (5.2 mg/m3), JUL2008
Occupational Exposure Limit-AUSTRIAMAK-TMW 0.5 ppm (5 mg/m3), 2007
Occupational Exposure Limit-BELGIUMtime-weighted average 0.5 ppm (5.3 mg/m3), Skin, MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 0.5 ppm (5 mg/m3), skin, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 0.5 ppm (5.2 mg/m3), short term exposure limit 1.5 ppm (16 mg/m3), skin, NOV2011
Occupational Exposure Limit-FRANCEVME 0.5 ppm (5 mg/m3), Skin, FEB2006
Occupational Exposure Limit-ICELANDtime-weighted average 0.5 ppm (5 mg/m3), skin, NOV2011
Occupational Exposure Limit-JAPANOccupational Exposure Limit 1 ppm (10.3 mg/m3), MAY2012
Occupational Exposure Limit-KOREAtime-weighted average 0.5 ppm (5 mg/m3), skin, 2006
Occupational Exposure Limit-MEXICOtime-weighted average 0.5 ppm (5 mg/m3) (skin), 2004
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 0.5 ppm (5.2 mg/m3), skin, JAN2002
Occupational Exposure Limit-PERUtime-weighted average 0,5 ppm (5,2 mg/m3), JUL2005
Occupational Exposure Limit-POLANDtime-weighted average 5 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAshort term exposure limit 5 mg/m3, JUN2003
Occupational Exposure Limit-SWITZERLANDMAK-week 0.5 ppm (5 mg/m3), carc 3, skin, JAN2011
Occupational Exposure Limit-THE NETHERLANDSMAC-TGG 5 mg/m3, Skin, 2003
Occupational Exposure Limit-THE PHILIPPINEStime-weighted average 0.5 ppm (5 mg/m3), Skin, JAN1993
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 0.5 ppm (5 mg/m3) (skin)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 0.5 ppm (5 mg/m3) (skin)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 0.5 ppm (5 mg/m3) (skin)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 0.5 ppm (5 mg/m3) (skin)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO BROMOFORM-air10H time-weighted average 0.5 ppm (Sk)NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code 13160; Number of Industries 1; Total Number of Facilities 14; Number of Occupations 2; Total Number of Employees Exposed 1473; Total Number of Female Employees Exposed 230
National Occupational Hazard Survey 1974Hazard Code 13160; Number of Industries 3; Total Number of Facilities 43; Number of Occupations 3; Total Number of Employees Exposed 242

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/91/180323/AS)
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Studies (gavage);clear evidence:rat
NCI Carcinogenesis Studies (gavage);no evidence:mouse
NIOSH Analytical Method, 1994: Hydrocarbons, halogenated, 1003
On EPA IRIS database

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