Difflugia

Difflugia
Races of Difflugia. (After Leidy.)
Scientific classification
Domain:
Eukaryota
(unranked):
Amoebozoa
Class:
Tubulinea
Order:
Arcellinida
Suborder:
Difflugina
Family:
Difflugiidae
Genus:
Difflugia

Leclerc, 1815

Difflugia is the largest genus of Arcellinida, one of several groups of Tubulinea within the eukaryote supergroup Amoebozoa.[1] Arcellinida species produce shells or tests from mineral particles or biogeonic elements (e.g. diatom frustules) and are thus commonly referred to as testate amoebae or shelled amoebae. Difflugia are particularly common in marshes and other freshwater habitats.

History

The genus Difflugia was initially discovered in 1815 by L, Leclerc,[2] but its infra-generic classification as a group is still unclear. The genus Difflugia is the oldest and most diverse of the testate amoebae. It contains more than 300 species and countless subspecies since even minor differences in morphology result in classification as a new species. In 1958, Gauthier-Lièvre and Thomas divided the genus into 10 groups depending on difference in shell morphology. These 10 groups are based on a survey conducted of the African species of Difflugia [3] The 10 shell shape classifications are lobed, collared, compressed, urceolate, globose, ovoid-globose, elongate, acute angled, horned and pyriform.

Taxonomy

There have been issues coming to a specific consensus about the taxonomy of the genus Difflugia. In 1988, an attempt to characterize and create a phylogenetic tree based upon the composition of the shell.[4] However, with additional evidence it was found that this is not actually a useful characteristic to evaluate by. In addition, most initial descriptions of Difflugia speciation were completed using light microscopy and therefore lacked the technology to show the details of test structure and composition.[5] The invention of scanning electron microscopy allows for more detail and improved the taxonomic work surrounding Difflugia. Studies surrounding Difflugia have been centered around species found mostly in Europe and Africa. [5] Molecular phylogenetic analyses have revealed that the genus Difflugia, as traditionally defined, is not a monophyletic group.[6] As a result, several globular and oviform species have been transferred to the genus Netzelia.[7]

External video
video icon Testate Amoebas: Blobby, Modest Shell Dwellers – Journey to the Microcosmos

Structure

Difflugia are testate amoebae with an agglutinate shell. The test can be composed of various different mineral particles depending on the varying environment. These mineral particles are collectively called xenosomes. All species of the genus Difflugia acquire their xenosomes, from the environment in which they inhabit. Difflugia often have species specific tests, which are arranged based on the specific shape and size. Most species of Difflugia only contain one nucleus but there are a select few that are multinucleated. In the larger species the nucleus can be vesicular as opposed to the regularly ovular nucleus. All species also contain an epipodium which is a terminal aperture in the protist's structure. The size of Difflugia is found to range from 15μm all the way up to 500μm.

During cell division, the particles collected that have been used to produce the test are directly passed on to the daughter cell. Strength of the test is quite variable between the Difflugia species and the strength of the test can be separated into three categories; robust, intermediate and fragile. Robust tests are found to result in a shell with an irregular shape in comparison to the rest of the genus. Shells which are a part of the fragile category are most likely to be constructed from diatom crystals.

Habitat and ecology

The environment conditions in which Difflugia lives is important to consider as it greatly influences shell morphology of the species. Shell composition depends on environmental pH and what materials are available. Difflugia can be found living in a large variety of habitats such as freshwater sediments, dry mosses and soil, or lakes. Some species are planktonic and switch to a benthic phase during the winter in which they stay immobile near the bottom of the body of water. One way environment can influence characteristics of Difflugia is that there are several freshwater species, which have green endosymbionts or zoochlorellae. The species Difflugia that contain endosymbionts are not considered to be heterotrophs, but instead mixotrophs because they combine being autotrophic and heterotrophic. The source of food depends on the environment but across the genus it is found to be mainly algae and fungi. Smaller species of Difflugia are found to consume bacteria as well as algae and fungi.

Fossil history

The presence of a test in Difflugia allowed them to be extremely well-preserved in fossils.[8] Difflugia are part of one of the oldest lineages of eukaryotes based on fossile records which date back 750Mya. The only structure of the organisms that was able to be preserved in the fossils was the test. Therefore there have been issues differentiating genera such as Cryptodifflugia from Difflugia based on fossil record alone.[9] Another problem arise from the distortion of test and changes to the xenosomes that makes genera difficult to identify from the testate amoebae fossil specimen alone.

References

  1. ↑ Adl SM, Simpson AG, Lane CE, Lukeš J, Bass D, Bowser SS, Brown MW, Burki F, Dunthorn M, Hampl V, Heiss A, Hoppenrath M, Lara E, le Gall L, Lynn DH, McManus H, Mitchell EA, Mozley-Stanridge SE, Parfrey LW, Pawlowski J, Rueckert S, Shadwick L, Schoch CL, Smirnov A, Spiegel FW (2012). "The Revised Classification of Eukaryotes". Journal of Eukaryotic Microbiology. 59 (5): 429–514. doi:10.1111/j.1550-7408.2012.00644.x. PMC 3483872. PMID 23020233.
  2. ↑ Mazei, Yuri; Warren, Allen (2012). "A survey of the testate amoeba genus Difflugia Leclerc, 1815 based on the specimens in the E. Penard and C. G. Ogden collections of the Natural History Museum, London. Part 1: Species with shells that are pointed aborally and/or have aboral protuberances". Protistology (7): 121–171.
  3. ↑ Snegovaya, N. Yu.; Tahirova, E. N. (2015). "A new species of testate amoebae in the genus Difflugia from the freshwaters of Azerbaijan (Rhizopodia, Testacea, Difflugiidae)". The Journal of National Academy of Sciences of Ukraine. 49 (2).
  4. ↑ Gomaa, Fatma; Todorov, Milcho; Heger, Thierry; Mitchell, Edward; Lara, Enrique (2012). "SSU rRNA Phylogeny of Arcellinida (Amoebozoa) Reveals the Largest Arcellenid Genus, Difflugia Leclerc 1815, is Not Monophyletic". Protist. 163 (3): 389–99. doi:10.1016/j.protis.2011.12.001. PMID 22326855.
  5. 1 2 Lahr, D. J.; Lopez, S. G. (2006). "Morphology, Biometry, Ecology and Biogeography of Five Different Species of Difflugia Leclerc, 1815 (Arcellinida: Difflugiidae), from Tiete River, Brazil" (PDF). Acta Protozoologica.
  6. ↑ Gomaa, Fatma; Todorov, Milcho; Heger, Thierry J.; Mitchell, Edward A. D.; Lara, Enrique (2012-05-01). "SSU rRNA Phylogeny of Arcellinida (Amoebozoa) Reveals that the Largest Arcellinid Genus, Difflugia Leclerc 1815, is not Monophyletic". Protist. 163 (3): 389–399. doi:10.1016/j.protis.2011.12.001. ISSN 1434-4610. PMID 22326855.
  7. ↑ Gomaa, Fatma; Lahr, Daniel J. G.; Todorov, Milcho; Li, Jingchun; Lara, Enrique (2017-06-01). "A contribution to the phylogeny of agglutinating Arcellinida (Amoebozoa) based on SSU rRNA gene sequences". European Journal of Protistology. 59: 99–107. doi:10.1016/j.ejop.2017.03.005. ISSN 0932-4739. PMID 28433921.
  8. ↑ Fiz-Palacios, O; Leander, B.S.; Heger, T.J. (2014). "Old Lineages in a New Ecosystem: Diversification of Arcellenid Amoebae (Amoebozoa) and Peatland Mosses". PLOS ONE. 9 (4): e95238. Bibcode:2014PLoSO...995238F. doi:10.1371/journal.pone.0095238. PMC 3999201. PMID 24762929.
  9. ↑ Charman, Dan J. (2001). "Testate amoebae and the fossil record: issues in biodiversity". Journal of Biogeography. 26 (1): 89–96. doi:10.1046/j.1365-2699.1999.00286.x.
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