Endo agar

Endo agar

Endo agar (also called Endo's medium) is a microbiological growth medium with a faint pink colour.[1] Originally developed for the isolation of Salmonella typhi, it is now used mostly as a coliform medium. Most gram-negative organisms grow well in this medium, while growth of gram-positive organisms is inhibited.[2] Coliform organisms ferment the lactose in this medium, producing a green metallic sheen (i.e. Escherichia coli), whereas non-lactose-fermenting organisms produce clear, colourless colonies,[1] i.e. Salmonella sp..

Typical composition

Endo agar typically contains (w/v):[3]

  • 1.0 % peptone
  • 0.25 % dipotassium hydrogen phosphate (K2HPO4)
  • 1.0 % lactose
  • 0.33 % anhydrous sodium sulfite (Na2SO3)
  • 0.03 % fuchsine
  • 1.25 % agar

References

  1. 1 2 "Mondofacto". Archived from the original on 2016-03-03. Retrieved 2009-04-25.
  2. ↑ Becton, Dickinson and Company, , 2006
  3. ↑ EMD Chemicals, "Archived copy". Archived from the original on 2009-04-13. Retrieved 2009-04-25.{{cite web}}: CS1 maint: archived copy as title (link), 2002
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