List of instruments used in microbiological sterilization and disinfection

Instruments used in microbiological sterilization and disinfection are as follows:[1][2][3]

Instrument list

InstrumentUses
Instrument sterilizersUsed to sterilize instruments in absence of an autoclave
Dressing drumsstorage of gowns, cotton, linen, etc.
The microscopeused for visualising minute structures including microbes
Various stainsused to stain microscopic slides to get contrast
Hot air ovenused in sterilizing instruments for various aseptic procedures, specially if that can not be autoclaved like powders
Koch's or Arnold's steam sterilizerused for steam sterilization
A pressure cookerused as a portable autoclave
Biological and chemical indicatorsUsed to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done
Filters:
Candle filter:used as household water filters and as filters for large particles in the laboratories
••Diatomaceous earth filters like the Berkefeld filter-do-
••Unglazed porcelain filters like the Chamberland filter-do-
•Disk filter or Seitz filterpreviously used as bacteriological filters; presently obsolete
•Sintered glass filterused as a good particle filter in laboratories
•Membrane filter and Syringe filterused as primary bacterial/cell filters in procedures as toxin, immunoglobulin, etc. production, where the product gets denatured on heating
•Air filterlike HEPA filter, used in various laboratories and clean rooms to produce lamellar air flow
Radiation:
•Gamma ray sourceused in sterilization of heat-labile products like plastic or rubber syringes, catheters and gloves
X-ray source-do-
•Infrared light source-do-
•Ultraviolet light source-do-
Inspissatorused to produce culture media for bacteriology that contain egg or serum, which coagulate on heating
Tyndallizera process of sterilization from spore bearing bacteria; video link
Water bathto heat things uniformly from all sides at a set temperature up to the boiling point of water
Needle DestroyerBurns the needle electrically either cuts the syringe manually or burns it electrically
Sharps container[4]A imperforable container for sharp wastes like needles, blades, microscope slides, broken glass, etc.
Cardboard biomedical waste containers
Reusable tubs[5]
Colour coded biomedical waste bags (India) [6]-
•Yellow plastic bagsfor human anatomical, animal, microbiological and soiled waste
•Red disinfected container or plastic bagsmicrobiological waste, solid waste(IV tubes, catheters, etc.)
•Blue or White plastic bag or puncture proof containerssharps, disposable tubing, etc.
•Black Plastic bag or puncture proof containersdiscarded medicines, incineration ash, chemical waste
Disinfectantsfor cleaning
Incineratorsto burn biomedical wastes like removed body parts, blood, gauze, linen, etc.

References

  1. Textbook of Microbiology by Prof. C P Baveja, ISBN 81-7855-266-3
  2. Textbook of Microbiology by Ananthanarayan and Panikar, ISBN 81-250-2808-0
  3. Biological Waste
  4. http://rules.sos.state.ga.us/docs/391/3/4/15.pdf.
  5. "Archived copy". Archived from the original on 2010-11-06. Retrieved 2014-01-07.{{cite web}}: CS1 maint: archived copy as title (link)
  6. 322_323
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