KIAA0895

The KIAA0895 gene is located at 7p14.2.[6] The genomic DNA is 65,976 base pairs long,[8] while the longest mRNA that it produces is 4463 bases long.

It can be transcribed into 15 transcript variants, which in turn can produce 13 different isoforms of the protein.[9]

The location of the KIAA0895 gene on chromosome 7

KIAA0895 is surrounded by the following genes on chromosome 7:[8]

• EEPD1
• MARK2P7
• ANLN
• LOC111365168
• AOAH

The gene encoded for the KIAA0895 protein is 65,975 nucleotides long, from nucleotides 36324150 to 36390125, with seven exons.

KIAA0895 is a lysine and arginine semi-enriched protein.[12] KIAA0895 is semi-enriched in positively charged lysine and arginine groups, and positively and negatively charged lysine, arginine, glutamic acid and aspartic acid groups.[12] However, KIAA0895 is semi-depleted in non-polar alanine, glycine and proline groups.[12]

The charge distribution analysis shows that there are no negative or mixed charge clusters.[12] However, there is one positive charge cluster from amino acids 12 to 36.[12]

LOC23366 contains a protein domain of unknown function called DUF1704.[13] It also contains a region of low complexity from position 120 to position 150 in the protein,[14] and an arginine-rich area from position 12 to position 51.[15]

Using ElDorado by Genomatrix, a promoter region sequence was found.[16] The most likely promoter for KIAA0895 starts at 36389926 and goes to 36391149, with a length of 1224.[16]

The predicted serine, threonine, and tyrosine phosphorylation sites of the KIAA0895 protein

KIAA0895 is predicted to undergo phosphorylation at several serines, threonines, and tyrosines throughout its structure.[17] Phosphorylation at these sites is a form of gene regulation. Phosphorylation results in a conformational change in the structure of many enzymes and receptors. This causes them to become activated or deactivated.

Using a database called Mfold, a stem-loop formation for the 5' UTR region shows that there is a lack of conservation, meaning there is some precedence that these stems and loops are not used for translation regulation.[18] The ΔG value was -12.90 kcal/mol with three loops.[18] Using the same database, a stem-loop formation for the 3' UTR region shows that there is conservation, meaning there is some precedence that they are used for translation regulation.[18] The ΔG value was -636.80 kcal/mol.[18]

KIAA0895 has a tertiary structure with alpha helices and beta sheets.[19]

Proposed Tertiary Structure for KIAA0895.

Proposed Tertiary Structure for 24% of KIAA0895. Image coloured by rainbow N → C terminus.

There are three proteins likely to be interacting proteins with KIAA0895. These proteins are ELAVL1,[20] vata,[21] and glym.[21] These interactions have experimental evidence from the sources provided.

Using an EST profile from NCBI, KIAA0895 has strong expression in cervical tumors and bladder carcinoma.[22]

An EST profile was created by NCBI. EST profiles show approximate gene expression patterns as inferred from EST counts and the cDNA library sources.