Night Shift Nurses

Ryuji Hirasaka (比良坂 竜二, Hirasaka Ryūji)

Voiced by: Nobuo Tobita (Japanese); Bjorkin Stiles, Michael Sanderson (English)

Ryuji Hirasaka is the aforementioned villain protagonist of the series. A knowledgeable practitioner, his life takes a sharp turn when he is invited to direct and corrupt a team of nurses at his new place of work. An unconvicted sex offender, Ryuji is vicious, controlling and intimidating, often cold reading those under him to learn about their vulnerabilities and how to exploit them. He sports a mullet and is bespectacled. He is murdered by Ren at the end of episode 5. In Night Shift Nurses Kranke he has a better ending: he marries Ren and the couple decide to move to the countryside to continue their experiments.

Narumi Jinguji (神宮寺 成美, Jinguji Narumi)

Voiced by: Kaoru Hoshioki (Japanese); Irie, Marcia Belle, Likki Dee Split (English)

Narumi Jinguji is the thirty-two-year-old[8] head practitioner of the St. Juliana Hospital, specializing in gynaecology. Voluptuous and enticing, she arranges to meet with Ryuji one afternoon to offer him the chance to sexually discipline a select group of her nursing staff for the purpose of developing a prostitution ring. As a previous rape victim of her own employee, Narumi exhibits bursts of psychological imbalance and unrest. She is noted for her long green hair. Like Ryuji, she too wears glasses. She commits suicide after attempting to kill Ryuji near the end of episode 5. In Night Shift Nurses Kranke, she is shown attending Ryuji and Ren's wedding.

Ren Nanase (七瀬 恋, Nanase Ren)

Voiced by: Yuu Mikage (Japanese); Lynna Dunham, Kendall Harrison (English)

Bright and friendly, Ren Nanase is a twenty-two-year-old[8] nurse at the St. Juliana Hospital who is arguably the most iconic character of the series. She is the first nurse Ryuji meets and the first who genuinely welcomes him as a friend and superior. When her love for an inpatient boxer, Naoya Ohkawa, is discovered, Ryuji learns of her match fixing and uses it as blackmail. Like the nurses to come, in spite of the abhorrent treatment she receives, Ren develops Stockholm syndrome and becomes emotionally attached to her abuser. Ren is distinguishable by her incredibly long hair, of which is usually tied into a ponytail. It is later revealed that she is pregnant. She is the one who kills Ryuji after he gained back his consciousness. After being arrested she admits that she loves him. In Night Shift Nurses Kranke, she marries Ryuji, lives with him in the countryside, and becomes a "good" wife.

Remi Shinjyo (新城 礼美, Shinjō Remi)

Voiced by: Nana Aikawa (Japanese); Nursey, Lotus, Jessica Thorne (English)

Remi Shinjyo is a twenty-seven-year-old[8] senior nurse of the hospital maternity ward. As a headstrong superior, Remi is outwardly proud, fastidious and authoritative, especially to Ryuji. Although it is not mentioned until later, Remi is responsible for an act of malpractice that resulted in the death of a patient. Ryuji takes advantage of Remi's malpractice and subjects her to inhumane experiments. Like Ren, Remi falls for Ryuji and shows huge interest of wanting to be experimented by Ryuji again. Amongst all the nurses, she is one of the few nurses in the series that is not a virgin.

Ako Fujisawa (藤沢 亜子, Fujisawa Ako)

Voiced by: Saya Tohno (Japanese); Alexis J. Parker, Kay Parrott, Mika Moon (English)

Shy and quiet, Ako Fujisawa is a twenty-three-year-old[8] pharmacist. The daughter of a wealthy family, she is falsely accused of stealing drugs from her department and this is used as the basis for her series of abuse. In much the same fashion as Ren and Remi, Ako develops delusional, obedient feelings for Ryuji. Owing to one of the elements of the show, Ako becomes a coprophile.

Hikaru Kodama (児玉 ひかる, Kodama Hikaru)

Voiced by: Mai Akizuki (Japanese); Syndi Snackwell (English)

Hikaru Kodama is a twenty-one-year-old[8] amateur nurse, newly employed and specializing in pediatrics. As her department suggests, Hikaru absolutely loves children and like them, is energetic. She is the fourth and final nurse to be subjected to Ryuji and by far endures the most inhumane practices in the first phase of the series. It is later revealed that most of the group has to endure the same thing as her. Hikaru is noted for her pigtails and loose socks. She has an adoptive sister, Ai, hospitalized for heart problems. She later admits to have fallen for the doctor. In Night Shift Nurses Kranke, the doctor later does his experiments on both her and her sister. During Ryuji and Ren's wedding, she is the one who catches the bouquet.

Ai Kodama (児玉 あい, Kodama Ai)

Ai Kodama is Hikaru's adopted younger sister and a female patient at St. Juliana Hospital who suffers from a serious heart condition. She has a very close relationship with her older sister.

Voiced by: Asuka Tanii, Mai Akizuki

Dr. Sekigawa (関川, Sekigawa-sensei)

Voiced by: Katsuyuki Konishi

Naoya Okawa (大川 直哉, Okawa Naoya)

Naoya is a professional boxer and a male patient at St. Juliana Hospital who had broken his leg in an accident. He has romantic feelings for Ren, until he found out that she was in a relationship with Ryuji.

Souichiro Kuwabara (九羽原 宗一郎, Kuwabara Souichiro)

Voiced by: Shinji Kawada

Souichiro Kuwabara is a young, seemingly kind-hearted male doctor supposedly in his late 20s who first appears in the second series of Night Shift Nurses-(Night Shift Nurses 2, Yakin Byoutou Ni|夜勤病棟・弐). A few years after St. Juliana closed down due to the strange circumstances that had occurred in the previous series, he gets employed at a different hospital called Sei-Katorea General Hospital-(聖カトレア総合病院|Sei Katorea Sōgō Byōin). Dubbed "The Super Elite Doctor", he gets a lot of admirers from the female staff and a few female patients; his father is a director of two other hospitals that he runs at the same time. Despite appearing to be kind-hearted at first, it turns out to be a facade, and he is revealed to be every bit as depraved and misogynistic as Ryuji Hisakawa, as he starts raping and abusing his female co-workers at the hospital.

Mana Kazama (風間 愛, Kazama Mana)

Voiced by: Nana Nogami (PC game) Emi Motoi (OVA)

Mana is a red-haired nurse employed at the same hospital as Souichiro.

Riuru Kaneshiro (金城 瑠璃, Kaneshiro Riuru)

Voiced by: Asuka Hojo

Riuru is the blonde-haired director of Sei-Katorea General Hospital. She's in a bisexual relationship with Konohara, one of the nurses employed at the hospital; it's revealed that she is futanari.

Karen Konohara (小乃原 香蓮, Konohara Karen)

Voiced by: Maria Asano (PC game) Mari Tomokawa (OVA)

Karen is a blue-haired lesbian nurse at Sei-Katorea General Hospital. She is shown to have a bad temper and has the personality of a typical tsundere character.

Suzuka Nogami (野上 涼華, Nogami Suzuka)

Voiced by: Ayana Sumoto

Suzuka is a lavender haired nursed at Sei-Katorea General Hospital. She is shown to have a habit of shoplifting random merchandise from different stores which she had inherited from her childhood; she has the personality of a young innocent child.

Sayuki Tezuka (手塚 咲雪, Tezuka Sayuki)

Voiced by: Yuki Iwata

Sayuki is an orange-haired nurse at Sei-Katorea General Hospital. She wears her hair up in two side braids; she also suffers from a minor form of Autonomic Ataxia, causing her body to tremble in fear whenever her stress levels get too high.

Jiro Yotsuya (四谷 次郎, Yotsuya Jirō)

Yotsuya is a young novelist who resides in a rural small town village called Tokizaka.

Yuu Yagami (八神 優, Yagami Yuu)

Voiced by: Ayaka Kimura

Yuu is a dark pink haired nurse who works at the same hospital that Yotsuya was staying at after he was injured.

Reika Mikage (御影 麗佳, Mikage Reika)

Voiced by: Mari Oda

Sakurako Kujou (九条 桜子, Kujou Sakurako)

Voiced by: Ren Kashikura

Hiyori Kisaragi (如月 ひより, Kisaragi Hiyori)

Voiced by: Haruka Kurasawa

Shizune Yunagi (夕凪 静音, Yūnagi Shizune)

Voiced by: Miru Takakura

Koko Amamiya (甘宮 心, Amamiya Koko)

Voiced by: Natsu Asaka

Shizuru Hirasaka (比良坂 詩弦, Hirasaka Shizuru)

Voiced by: Himari

Maya Yumizuka (弓塚 麻耶, Yumizuka Maya)

Voiced by: Milk Kano

Eri Kedouin (祁答院 英莉, Kedouin Eri)

As a result of the unrestrained content of the series, Night Shift Nurses is usually regarded as one of, if not the most graphic hentai ever released. Several minutes of particularly unruly scenes were even banned from North American releases.[9] Industry aggregator Mania.com gave the series an F, citing that although the animation is "clean" and "slick", the quantity of edits made for the American release yields it one that "[...]I in no way [can] recommend."

• Official Yakin Byōtō game website (in Japanese)
• Official Yakin Byōtō OVA website (in Japanese)
• Night Shift Nurses at The Visual Novel Database
• Night Shift Nurses (anime) at Anime News Network's encyclopedia
• Night Shift Nurse: Yagami Yu (anime) at Anime News Network's encyclopedia