Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase

Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase (EC 2.4.1.287, arabinogalactan galactofuranosyl transferase 1, GlfT1) is an enzyme with systematic name UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-beta/4-beta-galactofuranosyltransferase.[1][2] This enzyme catalyses the following chemical reaction

2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol (overall reaction)
(1a) UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol UDP + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
(1b) UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
Rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase
Identifiers
EC no.2.4.1.287
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

This enzyme is isolated from Mycobacterium tuberculosis and M. smegmatis.

References

  1. Mikusová K, Belánová M, Korduláková J, Honda K, McNeil MR, Mahapatra S, Crick DC, Brennan PJ (September 2006). "Identification of a novel galactosyl transferase involved in biosynthesis of the mycobacterial cell wall". Journal of Bacteriology. 188 (18): 6592–8. doi:10.1128/JB.00489-06. PMC 1595490. PMID 16952951.
  2. Belánová M, Dianisková P, Brennan PJ, Completo GC, Rose NL, Lowary TL, Mikusová K (February 2008). "Galactosyl transferases in mycobacterial cell wall synthesis". Journal of Bacteriology. 190 (3): 1141–5. doi:10.1128/JB.01326-07. PMC 2223555. PMID 18055597.
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