Immunofluorescence

Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. The specific region an antibody recognizes on an antigen is called an epitope.[1] There have been efforts in epitope mapping since many antibodies can bind the same epitope and levels of binding between antibodies that recognize the same epitope can vary.[2] Additionally, the binding of the fluorophore to the antibody itself cannot interfere with the immunological specificity of the antibody or the binding capacity of its antigen.[3] Immunofluorescence is a widely used example of immunostaining (using antibodies to stain proteins) and is a specific example of immunohistochemistry (the use of the antibody-antigen relationship in tissues). This technique primarily makes use of fluorophores to visualise the location of the antibodies.[4]

Photomicrograph of a histological section of human skin prepared for direct immunofluorescence using an anti-IgA antibody. The skin is from a patient with Henoch–Schönlein purpura: IgA deposits are found in the walls of small superficial capillaries (yellow arrows). The pale wavy green area on top is the epidermis, the bottom fibrous area is the dermis.
These figures demonstrates the basic mechanism of immunofluorescence. Primary immunofluorescence is depicted on the left, which shows an antibody with a fluorophore group bound to it directly binding to the epitope of the antigen for which it is specific. Once the antibody binds to the epitope, the sample can be viewed under fluorescent microscope to confirm the presence of the antigen in the sample. Conversely, secondary immunofluorescence is depicted to the right, which shows that first an untagged primary antibody binds to the epitope of the antigen in a mechanism similar to the one described above. However, after the primary antibodies have bound to their target, a secondary antibody (tagged with a fluorophore) comes along. This secondary antibody's binding sites are specific for the primary antibody that's already bound to the antigen, and therefore the secondary antibody binds to the primary antibody. This method allows for more fluorophore-tagged antibodies to attach to their target, thus increasing the fluorescent signal during microscopy.

Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules. This technique can even be used to visualize structures such as intermediate-sized filaments.[5] If the topology of a cell membrane has yet to be determined, epitope insertion into proteins can be used in conjunction with immunofluorescence to determine structures.[6] Immunofluorescence can also be used as a "semi-quantitative" method to gain insight into the levels and localization patterns of DNA methylation since it is a more time-consuming method than true quantitative methods and there is some subjectivity in the analysis of the levels of methylation.[7] Immunofluorescence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used. Various super-resolution microscope designs that are capable of much higher resolution can also be used.[8]

Types
Photomicrograph of a histological section of human skin prepared for direct immunofluorescence using an anti-IgG antibody. The skin is from a patient with systemic lupus erythematosus and shows IgG deposit at two different places: The first is a band-like deposit along the epidermal basement membrane ("lupus band test" is positive). The second is within the nuclei of the epidermal cells (anti-nuclear antibodies).

Preparation of fluorescence

To make fluorochrome-labeled antibodies, a fluorochrome must be conjugated ("tagged") to the antibody. Likewise, an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique. Staining procedures can apply to both fixed antigen in the cytoplasm or to cell surface antigens on living cells, called "membrane immunofluorescence". It is also possible to label the complement of the antibody-antigen complex with a fluorescent probe. In addition to the element to which fluorescence probes are attached, there are two general classes of immunofluorescence techniques: primary and secondary. The following descriptions will focus primarily on these classes in terms of conjugated antibodies.[3]

There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect).

Primary (direct)

Primary (direct) immunofluorescence uses a single, primary antibody, chemically linked to a fluorophore. The primary antibody recognizes the target molecule (antigen) and binds to a specific region called the epitope. This is accomplished by a process which manipulates the immune response of organism with adaptive immunity. The attached fluorophore can be detected via fluorescent microscopy, which, depending on the messenger used, will emit a specific wavelength of light once excited.[9] Direct immunofluorescence, although somewhat less common, has notable advantages over the secondary (indirect) procedure. The direct attachment of the messenger to the antibody reduces the number of steps in the procedure, saving time and reducing non-specific background signal.[10] This also limits the possibility of antibody cross-reactivity and possible mistakes throughout the process. However, some disadvantages do exist in this method. Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is substantially less sensitive than indirect immunofluorescence and may result in false negatives. Direct immunofluorescence also requires the use of much more primary antibody, which is extremely expensive, sometimes running up to $400.00/mL.

Secondary (indirect)
A fluorescent stain for actin in the smooth muscle of the skin.

Secondary (indirect) immunofluorescence uses two antibodies; the unlabeled first (primary) antibody specifically binds the target molecule, and the secondary antibody, which carries the fluorophore, recognizes the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary antibody. This provides signal amplification by increasing the number of fluorophore molecules per antigen.[10] This protocol is more complex and time-consuming than the primary (or direct) protocol above, but allows more flexibility because a variety of different secondary antibodies and detection techniques can be used for a given primary antibody.[10]

This protocol is possible because an antibody consists of two parts, a variable region (which recognizes the antigen) and constant region (which makes up the structure of the antibody molecule). It is important to realize that this division is artificial and in reality the antibody molecule is four polypeptide chains: two heavy chains and two light chains. A researcher can generate several primary antibodies that recognize various antigens (have different variable regions), but all share the same constant region. All these antibodies may therefore be recognized by a single secondary antibody. This saves the cost of modifying the primary antibodies to directly carry a fluorophore.

Different primary antibodies with different constant regions are typically generated by raising the antibody in different species. For example, a researcher might create primary antibodies in a goat that recognize several antigens, and then employ dye-coupled rabbit secondary antibodies that recognize the goat antibody constant region ("rabbit anti-goat" antibodies). The researcher may then create a second set of primary antibodies in a mouse that could be recognized by a separate "donkey anti-mouse" secondary antibody. This allows re-use of the difficult-to-make dye-coupled antibodies in multiple experiments.

Limitations

As with most fluorescence techniques, a significant problem with immunofluorescence is photobleaching. Loss of activity caused by photobleaching can be controlled by reducing or limiting the intensity or time-span of light exposure, by increasing the concentration of fluorophores, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Some problems that may arise from this technique include autofluorescence, extraneous undesired specific fluorescence, and nonspecific fluorescence. Autofluorescence includes fluorescence emitted from the sample tissue or cell itself. Extraneous undesired specific fluorescence occurs when a targeted antigen is impure and contains antigenic contaminants. Nonspecific fluorescence involves the loss of a probe's specificity due to fluorophore, from improper fixation, or from a dried out specimen.[3]

Immunofluorescence is only limited to fixed (i.e., dead) cells when structures within the cell are to be visualized because antibodies do not penetrate the cell membrane when reacting with fluorescent labels. Antigenic material must be fixed firmly on the site of its natural localization inside the cell.[3] Intact antibodies can also be too large to dye cancer cells in vivo.[11] Their size results in slow tumor penetration and long circulating half-life. Research has been done investigating the use of diabodies to get around this limitation.[11] Proteins in the supernatant or on the outside of the cell membrane can be bound by the antibodies; this allows for living cells to be stained. Depending on the fixative that is being used, proteins of interest might become cross-linked and this could result in either false positive or false negative signals due to non-specific binding.

An alternative approach is using recombinant proteins containing fluorescent protein domains, e.g., green fluorescent protein (GFP). Use of such "tagged" proteins allows determination of their localization in live cells. Even though this seems to be an elegant alternative to immunofluorescence, the cells have to be transfected or transduced with the GFP-tag, and as a consequence they become at least S1 or above organisms that require stricter security standards in a laboratory. This technique involves altering the genetic information of cells.[12]

Advances

Many improvements to this method lie in the improvement of fluorescent microscopes and fluorophores. Super-resolution methods generally refer to a microscope's ability to produce resolution below the Abbe limit (a limit placed on light due to its wavelength). This diffraction limit is about 200-300 nm in the lateral direction and 500-700 nm in the axial direction. This limit is comparable or larger than some structures in the cell, and consequently, this limit prevented scientists from determining details in their structure.[13] Super-resolution in fluorescence, more specifically, refers to the ability of a microscope to prevent the simultaneous fluorescence of adjacent spectrally identical fluorophores.[14] This process effectively sharpens the point-spread function of the microscope.[13] Examples of recently developed super-resolution fluorescent microscope methods include stimulated emission depletion (STED) microscopy, saturated structured-illumination microscopy (SSIM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM).[15]

See also

References
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  2. Ladner RC (2007-01-01). "Mapping the epitopes of antibodies". Biotechnology & Genetic Engineering Reviews. 24 (1): 1–30. CiteSeerX 10.1.1.536.6172. doi:10.1080/02648725.2007.10648092. PMID 18059626. S2CID 34595289.
  3. Akiyoshi K (1983-01-01). Immunofluorescence in medical science : with 28 tab. Springer u.a. ISBN 978-3540124832. OCLC 643714056.
  4. "Immunofluorescence". Protocol Online.
  5. Franke WW, Schmid E, Osborn M, Weber K (October 1978). "Different intermediate-sized filaments distinguished by immunofluorescence microscopy". Proceedings of the National Academy of Sciences of the United States of America. 75 (10): 5034–5038. Bibcode:1978PNAS...75.5034F. doi:10.1073/pnas.75.10.5034. PMC 336257. PMID 368806.
  6. Wang H, Lee EW, Cai X, Ni Z, Zhou L, Mao Q (December 2008). "Membrane topology of the human breast cancer resistance protein (BCRP/ABCG2) determined by epitope insertion and immunofluorescence". Biochemistry. 47 (52): 13778–13787. doi:10.1021/bi801644v. PMC 2649121. PMID 19063604.
  7. Çelik S (January 2015). "Understanding the complexity of antigen retrieval of DNA methylation for immunofluorescence-based measurement and an approach to challenge". Journal of Immunological Methods. 416: 1–16. doi:10.1016/j.jim.2014.11.011. PMID 25435341.
  8. "Immunofluorescence Method". Davidson College.
  9. "Immunohistochemical Staining Methods" (PDF). IHC Guidebook (Sixth ed.). Dako Denmark A/S, An Agilent Technologies Company. 2013.
  10. Fritschy JM, Härtig W (2001). "Immunofluorescence". eLS. doi:10.1038/npg.els.0001174. ISBN 047001590X.
  11. Sonn GA, Behesnilian AS, Jiang ZK, Zettlitz KA, Lepin EJ, Bentolila LA, et al. (March 2016). "Fluorescent Image-Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time". Clinical Cancer Research. 22 (6): 1403–1412. doi:10.1158/1078-0432.CCR-15-0503. PMC 4794340. PMID 26490315.
  12. Chalfie M (October 1995). "Green fluorescent protein". Photochemistry and Photobiology. 62 (4): 651–656. doi:10.1111/j.1751-1097.1995.tb08712.x. PMID 7480149. S2CID 3944607.
  13. Huang B, Bates M, Zhuang X (2009-06-02). "Super-resolution fluorescence microscopy". Annual Review of Biochemistry. 78: 993–1016. doi:10.1146/annurev.biochem.77.061906.092014. PMC 2835776. PMID 19489737.
  14. Diaspro A, van Zandvoort MA (2016-11-03). Super-resolution imaging in biomedicine. ISBN 9781482244359. OCLC 960719686.
  15. Leung BO, Chou KC (September 2011). "Review of super-resolution fluorescence microscopy for biology". Applied Spectroscopy. 65 (9): 967–980. Bibcode:2011ApSpe..65..967L. doi:10.1366/11-06398. PMID 21929850.
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