Leuconostoc mesenteroides
Leuconostoc mesenteroides is a species of lactic acid bacteria associated with fermentation, under conditions of salinity and low temperatures (such as lactic acid production in fermented sausages).[3] In some cases of vegetable and food storage, it was associated with pathogenicity (soft rot, slime and unpleasant odor).[4] L. mesenteroides is approximately 0.5-0.7 µm in diameter and has a length of 0.7-1.2 µm,[3] producing small grayish colonies that are typically less than 1.0 mm in diameter. It is facultatively anaerobic, Gram-positive, non-motile, non-sporogenous, and spherical. It often forms lenticular coccoid cells in pairs and chains, however, it can occasionally form short rods with rounded ends in long chains, as its shape can differ depending on what media the species is grown on.[3][4] L. mesenteroides grows best at 30°C, but can survive in temperatures ranging from 10°C to 30°C. Its optimum pH is 5.5, but can still show growth in pH of 4.5-7.0.[4]
| Leuconostoc mesenteroides | |
|---|---|
| Scientific classification | |
| Domain: | Bacteria |
| Phylum: | |
| Class: | |
| Order: | |
| Family: | |
| Genus: | |
| Species: | L. mesenteroides |
| Binomial name | |
| Leuconostoc mesenteroides (Tsenkovskii 1878) van Tieghem 1878 [2] | |
| Synonyms | |
|
Ascococcus mesenteroides Tsenkovskii 1878 | |
Microbiological characteristics
L. mesenteroides is an obligate heterolactic fermentative lactic acid bacterium (LAB) that is mostly used in industrial dairy fermentation, playing various roles, such as production of dextran, gas, and flavor compounds.[4] It is approximately 0.5-0.7 µm by 0.7-1.2 µm, and produces small grayish colonies that are usually less than 1.0 mm in diameter.[3] L. mesenteroides is a facultative anaerobe and will undergo heterolactic fermentation under microaerophilic conditions.[4] Taking this into consideration, it is important to note that L. mesenteroides utilizes sugar glucose as its primary source of metabolism, also well as other sugars such as sucrose and fructose.[4] Then, it creates ethanol, lactate, and CO2 as products of fermentation.[4] When grown in sucrose solution, it converts the sugar to dextrans having mostly alpha 1,6 linkages, but 1,2, 1,3, and 1,4 linkages are also present.[3][4]
Environment
L. mesenteroides is typically found on the skin of a large variety of fleshy fruits and vegetables,[4] and can be cultured using MRS agar, tomato juice agar, MRS broth, and skim milk.[4] These common medias are not ideal for growth and specialized medias are needed to grow to a high cell density. This microbe is commonly used for souring vegetables like cucumbers and cabbage, producing fermented foods such as kim chi, sauerkraut, and pickles.[4] The bacteria is included in dairy starter cultures since they are able to produce metabolites needed for dairy production. These metabolites include diacetyl and CO₂ from citric acid. Diacetyl is important for dairy because it is the main source of aroma and flavor in many different dairy products, like buttermilk, butter, and different cheeses.[5] The production of CO₂ is important for the eye formation in many cheeses such as Havarti. Specialized media is commonly used for industrial growth because components in standard medias like MRS contain meat extract and peptone that is not Kosher or halal which is needed for many dairy and vegetable products. L. mesenteroides does best in temperatures ranging between 10°C to 30°C,[3][4] but has an optimum temperature of 30°C. Additionally, it can survive in a pH range of 4.5-7.0, with an optimum of 5.5-6.5.[6] L. mesenteroides also has a doubling time of 0.6 h−1 under aerobic conditions.[3][4]
Genetics
The genome of L. mesenteroides has been successfully mapped, having an average genome size of 1.90138 Mbp and 1762 protein genes, with a G+C content of 37.8%.[3] L. mesenteroides is from the phylum Bacillota, and is a member of the lactic bacteria family. This is important as it has the ability to produce lactic acid which lowers the pH of the surrounding environment and, in turn, inhibits other competing food spoilage organism's growth as they cannot tolerate the acidic environment.[3][4]
Health risks
Leuconostoc mesenteroides subsp. mesenteroides has been found to show pathogenic characteristics in rare cases. The first case of this infecting a human was in 1985. A more recent outbreak in a hospital in 2004 had 48 cases where it was found that penicillin and gentamicin could be used as antimicrobial treatment. Samples of the bacteria were isolated in both the patient's blood and urine.[7]
Taxonomy
L. mesenteroides is divided into several subspecies.
- L. m. subsp. cremoris (Knudsen and Sørensen 1929) Garvie 1983
- L. m. subsp. dextranicum (Beijerinck 1912) Garvie 1983
- L. m. subsp. mesenteroides (Tsenkovskii 1878) Garvie 1983
- L. m. subsp. suionicum Gu et al. 2012
Subspecies characteristics
Subsp. Mesenteroides
This strain can grow in NaCl up to 3.0% and some strains up to 6.5% and optimum temperature of 20 and 30℃. It is also been found that when growth occurs in milk with supplemental yeast extract and glucose, enough acid is produced to curdle milk. Some, but not all strains can ferment citrate. Different strains of Subsp. Mesenteroides tested also differ by their need for riboflavin, pyridoxal and folic acid, these need to be tested to determine a starting point for the media. Tween 80, uracil and combinations of uracil, adenine, and xanthine are not required for growth. Subsp. Mesenteroides also require glutamic acid and valine.[6]
Subsp. Cremoris
This strain grows best between 18 and 25℃.This strain can ferment citrate into acetoin and diacetyl. Most strains of this subspecies cannot ferment sucrose. Of the three subspecies, this ferments the least kinds of carbohydrates. All strains can ferment glucose and lactose; galactose and maltose is strain specific. This strain requires more nutrients, riboflavin, pyridoxal, folic acid, uracil and a combination of uracil, adenine, and xanthine are required.[6]
Subsp. Dextranicum
This strain is similar to Subsp. Mesenteroides, the optimum temperature of 20 and 30℃. It can also ferment Glucose, fructose, lactose, maltose, sucrose and trehalose. There is also a variation by strain for requirements of riboflavin, pyridoxal and folic acid. Some strains also required a combination of uracil, guanine, adenine and xanthine.[6]
References
- Zheng J, Wittouck S, Salvetti E, Franz CMAP, Harris HMB, Mattarelli P, O'Toole PW, Pot B, Vandamme P, Walter J, Watanabe K, Wuyts S, Felis GE, Gänzle MG, Lebeer S. (2020). "A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae". Int J Syst Evol Microbiol. 70 (4): 2782–2858. doi:10.1099/ijsem.0.004107. PMID 32293557.
- Page Leuconostoc on lpsn.dsmz.de
- Özcan E, Selvi SS, Nikerel E, Teusink B, Toksoy Öner E, Çakır T (April 2019). "A genome-scale metabolic network of the aroma bacterium Leuconostoc mesenteroides subsp. cremoris". Applied Microbiology and Biotechnology. 103 (7): 3153–3165. doi:10.1007/s00253-019-09630-4. hdl:1871.1/7a12c804-4c10-4aef-99ef-b72df2c365f4. PMID 30712128.
- Dols M, Chraibi W, Remaud-Simeon M, Lindley ND, Monsan PF (June 1997). "Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production". Applied and Environmental Microbiology. 63 (6): 2159–65. doi:10.1128/AEM.63.6.2159-2165.1997. PMC 168507. PMID 9172334.
- Kleppen HP, Nes IF, Holo H (September 2012). "Characterization of a Leuconostoc bacteriophage infecting flavor producers of cheese starter cultures". Applied and Environmental Microbiology. 78 (18): 6769–72. doi:10.1128/aem.00562-12. PMC 3426687. PMID 22798359.
- Björkroth J, Dicks LM, Holzapfel WH (2015-09-14). "Weissella". Bergey's Manual of Systematics of Archaea and Bacteria. John Wiley & Sons, Ltd. pp. 1–15. doi:10.1002/9781118960608.gbm00609. ISBN 978-1-118-96060-8.
- Bou G, Luis Saleta J, Sáez Nieto JA, Tomás M, Valdezate S, Sousa D, et al. (June 2008). "Nosocomial Outbreaks Caused by Leuconostoc mesenteroides subsp. mesenteroides". Emerging Infectious Diseases. 14 (6): 968–71. doi:10.3201/eid1406.070581. PMC 2600284. PMID 18507917.