Uterine microbiome

Evidence shows that the presence of uterine bacterial 16S rRNA gene amplification is not the result of sampling or analysis error and deserves to be acknowledged. Concept of the sterile endometrium, and the uterine compartment in general, is outdated; although the true core uterine microbiome still needs to be assessed, and may vary across cultural and regional divides. Functional studies are needed to elucidate the physiological importance of the microbiome in fertility. The challenge of studying reproductive immunology and the microbiota involved is that research on all of the different aspects is still in its infancy; the microbiome, immunity, and endocrinology in pregnancy need to be studied both locally and temporally with placental and fetal development to obtain a more comprehensive overview. [4]

Bacteria, viruses and one genus of yeasts are a normal part of the uterus before and during pregnancy.[5] The uterus has been found to possess its own characteristic microbiome that differs significantly from the vaginal microbiome, consisting primarily of lactobacillus species, and at far fewer numbers.[6]In addition, the immune system is able to differentiate between those bacteria normally found in the uterus and those that are pathogenic. Hormonal changes have an effect on the microbiota of the uterus.[7]

Prophylactic antibiotics have been injected into the uterus to treat infertility. This has been done before the transfer of embryos with the intent to improve implantation rates. No association exists between successful implantation and antibiotic treatment.[11] Infertility treatments often progress to the point where a microbiological analysis of the uterine microbiota is performed. Preterm birth is associated with certain species of bacteria that are not normally part of the healthy uterine microbiome.[5]

The immune response becomes more pronounced when bacteria are found that are not commensal.[5]

Investigations into reproductive-associated microbiomes began around 1885 by Theodor Escherich. He wrote that meconium from the newborn was free of bacteria. There was a general consensus at the time and even recently that the uterus was sterile and this was referred to as the sterile womb paradigm. Other investigations used sterile diapers for meconium collection. No bacteria were able to be cultured from the samples. Other studies showed that bacteria were detected and were directly proportional to the time between birth and the passage of meconium.[1]

Investigations into the role of the uterine microbiome in the development of the infant microbiome are ongoing.[1]

• Human microbiome
• Human Microbiome Project
• Human virome
• List of antimicrobial peptides in the female reproductive tract
• List of bacterial vaginosis microbiota
• Placental microbiome
• Vaginal epithelium
• Vaginal flora in pregnancy