Auramine phenol stain
Auramine phenol stain is a stain used in clinical microbiology and histology to identify tuberculosis mycobacteria.
There are two types of auramine phenol stains, 1 and 2 to stain mycobacterium species and cryptosporidium respectively. Both are fluorescent stains. The bacteria or the parasites appear brilliant greenish yellow against dark background.[1] Mycolic acids of the mycobacteria keep this stain when decolorising with the acid alcohol. The method is more rapid and sensitive than ZN technique.[2]
Method
- Smears are prepared just like that for ZN staining
- Stain with Auramine-Phenol for 20 mins
- Rinse with water
- Decolourise in acid alcohol
- Rinse with water
- Counterstain with 0.1% potassium permanganate for 30 seconds
- Rinse and air dry[3]
References
- ↑ "Microbiology training log". Retrieved 19 November 2012.
- ↑ "Comparison of the value of two different sputum staining for diagnosis of acid-fast bacilli". Retrieved 19 November 2012.
- ↑ "Parasitology stains". Archived from the original on 10 April 2011. Retrieved 19 November 2012.
| Symptoms, signs and associated conditions |  | |
|---|---|---|
| Mycobacterium species | ||
| Tuberculosis diagnosis | ||
| Management | ||
| Resistance | ||
| History of tuberculosis |
| |
| Organizations |
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| Other |
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