Partial thromboplastin time

Blue Top Vacutainer tube (sodium citrate vial) used for PT and PTT blood tests

Partial thromboplastin time is typically analyzed by a medical technologist or a laboratory technician on an automated instrument at 37 °C (as a nominal approximation of normal human body temperature). The test is termed "partial" due to the absence of tissue factor from the reaction mixture.

• Blood is drawn into a test tube containing oxalate or citrate, molecules which act as an anticoagulant by binding the calcium in a sample. The blood is mixed, then centrifuged to separate blood cells from plasma (as partial thromboplastin time is most commonly measured using blood plasma).
• A sample of the plasma is extracted from the test tube and placed into a measuring test tube.
• Next, an excess of calcium (in a phospholipid suspension) is mixed into the plasma sample (to reverse the anticoagulant effect of the oxalate enabling the blood to clot again).
• Finally, in order to activate the intrinsic pathway of coagulation, an activator (such as silica, celite, kaolin, ellagic acid) is added, and the time the sample takes to clot is measured optically. Some laboratories use a mechanical measurement, which eliminates interferences from lipemic and icteric samples.

The typical reference range is between 30 seconds and 50 s (depending on laboratory). Shortening of the PTT is considered to have little clinical relevance, but some research indicates that it might increase risk of thromboembolism.[3] Normal PTT requires the presence of the following coagulation factors: I, II, V, VIII, IX, X, XI and XII. Notably, deficiencies in factors VII or XIII will not be detected with the PTT test.

Prolonged aPTT may indicate:

• use of heparin (or contamination of the sample)
• antiphospholipid antibody (especially lupus anticoagulant, which paradoxically increases propensity to thrombosis)
• coagulation factor deficiency (e.g., hemophilia)
• sepsis — coagulation factor consumption
• presence of antibodies against coagulation factors (factor inhibitors)

To distinguish the above causes, mixing tests are performed, in which the patient's plasma is mixed (initially at a 50:50 dilution) with normal plasma. If the abnormality does not disappear, the sample is said to contain an "inhibitor" (either heparin, antiphospholipid antibodies or coagulation factor specific inhibitors), while if it does disappear a factor deficiency is more likely. Deficiencies of factors VIII, IX, XI and XII and rarely von Willebrand factor (if causing a low factor VIII level) may lead to a prolonged aPTT correcting on mixing studies.

The aPTT is usually normal in pregnancy but tends to slightly decrease in late pregnancy.[4]

The aPTT-based activated protein C (APC) resistance test is used in the diagnosis of APC resistance (APCR).[5] It involves a modified aPTT test performed in the presence and absence of APC.[5][6] The ratio of these aPTT values is calculated and is called the APC sensitivity ratio (APCsr) or simply APC ratio (APCr).[5][6] This ratio is inversely related to the degree of APC resistance.[7] The aPTT-based APC resistance test was developed in 1993.[6]

The aPTT was first described in 1953 by researchers at the University of North Carolina at Chapel Hill.[8] This is the reason why the color of the Vacutainer tube top is Carolina blue.

• Thromboplastin