Corynebacterium striatum

API Strip. A series of standardised and controlled biochemical tests used in the identification of specific bacterium species.

Standardised methods of identification have been developed to improve the identification and isolation of Coronyforms. One such method is the API Coryne V2.0 system.[12] API strips combine a series of small scale biochemical tests to distinguish key characteristics of bacterium, based on metabolic activity.[13] The results are interpreted via standardised indicators and charts.[13] This is a cost-effective yet time-consuming method of identification, taking approximately 16 hours, particularly in clinical settings.

More modern identification techniques include genome sequencing – particularly 16S Ribosomal RNA (rRNA) Sequencing.[12] This technique relies on computerised comparison of genome sequences between bacterium.[14] The 16S rRNA sequence is highly conserved between bacterium, although will show slight variations and mutations between strains.[14] Comparison of variations in the Corynebacterium genome allow for specific identification of the Corynebacterium Striatum. It must be noted that currently this is currently a relatively expensive method of identification – when compared to the API strip, although this is improving as technologies improve.[14] The MALDI-TOF system is a clinically relevant method of detection that provides a rapid, 10 minute specific identification of bacteria.[12]

Both biochemical and molecular identification is accompanied by physical characterisation. Culturing the bacteria on blood cultures and gram staining to confirm morphology are integral additions to the process of identification.[11]

Corynebacterium diphtheriae Gram stain. The purple colour of the bacterium is indicative of the thin peptidoglycan wall surrounding the bacterium. The 'club-like' structure can be observed. This an example of a Coryneform bacterium.

Corynebacterium striatum is a gram-positive bacterium, meaning they have a thin external peptidoglycan cell wall structure.[1][15] They have been described as having an irregular pleomorphic shape, and are non-motile.[16] Under a microscope they appearing as a hybrid of a bacillus and cocci morphology with a bulged pole attached to rod like end, more commonly described as a 'club-like' structure.[1][17] They are broadly described as being 1.5-8.0 micrometers in length, but formal measurement of individual clinical isolates will vary upon observation.[17] C. striatum colonies are able to be plated in vitro; when growing on Blood Agar the colonies will appear as small (1-2mm diameter) with a white, moist, and smooth appearance.[1][18] It is otherwise called a diphtheroid or coryneform due to its close phylogenetic relationship with diphtheria causing bacterium Corynebacterium diphtheriae.[4]

Corynebacterium striatum can be differentiated from other Corynebacterium types based on its ability to ferment glucose and sucrose, and inability to ferment maltose.[1][16] In comparison to other members of the genus Corynebacterium, it ferments sugars rapidly.[5] Corynebacterium species are also known to ferment nitrates.[16] It is also able to hydrolyse Tyrosine and Pyrazinamide. It does not metabolize urease, can hydrolyze esculin, and can also ferment mannitol and xylose.[5] In a laboratory setting, it is best distinguished from other coronyforms through its fermentative activity. They are non-lipophilic preferring to persist

The organism itself possesses few virulence factors, giving it the title of an opportunistic colonizer as opposed to a true pathogen.[7] Virulence arises from its antibiotic resistance properties.[1] The increasing acquisition of antibiotic resistance genes allow for pathogenesis of Corynebacterium striatum colonisation.[1][6]

Many cases of infection with Corynebacterium striatum have been documented with particular relevance to acquisition of infection in hospitals – otherwise known as a nosocomial infection.[22][3] Corynebacterium striatum has been known to colonise prosthetics, particularly heart valves, prosthetic joints and even intravenous apparatus such as catheters.[7] Infections of this type have been described as a local infection or they can progress into a larger disseminated infection otherwise known as bacteraemia. Other documented infections include osteomyelitis, an infection of the bone that can occur through blood born infection or injury to the bone itself.[4][24]

One of the first described infections of an individual with Corynebacterium striatum occurred in 1980.[25] The man was severely immunocompromised, suffering already from leukemia.[25] After this, a number of isolated cases were documented. A well documented outbreak, at the Hospital Joan March, Mallorca, Spain, saw 21 individuals infected with Corynebacterium striatum.[22] The individuals all suffered from chronic obstructive pulmonary disease (COPD), and had also had significant tobacco exposure through their life, and as such were consistently being admitted to the hospital where they were treated by care staff with shared equipment.[22] In this instance Corynebacterium striatum was causing infection in the respiratory tract of patients and detected in sputum samples.[22] Of the 21 cases in the outbreak, there were six associated deaths.[22]

Another study of infection with Corynebacterium striatum described a patient who was admitted to hospital for treatment of cardiac arrest in 2008.[3] During her stay the patient was fitted with a central venous catheter through which she contracted bacteraemia of Corynebacterium striatum which resulted in her death.[3] The patient's age and immuno-compromised state resulting from pre-existing kidney failure ultimately allowed for the establishment and dissemination of infection.[3]

There is little to no evidence for Corynebacterium Striatum infection or colonising animal specimens.

Multidrug resistance is the main factor considered when treating disease caused by Corynebacterium striatum.[19][6] Treatment with a mix of broad-spectrum antibiotics may thus be necessary. The selection of further antibiotic resistance is a serious consideration that must be made when selecting patient treatment. A study into the occurrence of non-diphtheroid infections determined that of all bacterium tested, Corynebacterium Striatum had the highest occurrence, accounting for 47% of infections.[26] In the same study, it was determined that all non-diphtheroid corynebacterium were susceptible to treatment with vancomycin, quinupristin-dalfopristin, linezolid and gentamicin.[26] While multiple studies confirmed that vancomycin was highly effective on all isolated species.[10][27] Previously it was known that Coronybacterium were susceptible to β-lactams, tetracycline, and fluoroquinolones, but recently, resistance genes to such treatments have been observed in clinical isolates.[27] Similar resistance was noted in a study with all isolates showing resistance to ciprofloxacin.[10] Of significant clinical significance is a rising resistance to beta-lactams in the last two decades, a group of antibiotics that includes penicillin.[28] Suggested oral treatments include a class of oxazolidinones - linezolid.[27] Although treatment with linezolid is not often prescribed as sustained treatment can affect liver function and have negative side effects including headaches and nausea.[29] Although differing results of susceptibility to antibiotic treatment has been noted between experiments, as such specific susceptibility of isolates should be obtained through antibiotic sensitivity testing. In clinical situations the antibiotic sensitivity can be obtained through disk diffusion assays of E-strip test. Treatment of clinical cases, such as the outbreak at the Hospital Joan March, were treated case by case, based on the antibiogram obtained from each patient's sputum sample.[22]

More broadly, prevention of initial infection, particularly in a hospital setting, is a key element of treatment. Sterilising all surfaces, and prostheses, as well as constant replacement and maintenance of prosthesis, is an integral element of stopping disease establishment.[30]