Anaerobic respiration

Anaerobic respiration is respiration using electron acceptors other than molecular oxygen (O2). Although oxygen is not the final electron acceptor, the process still uses a respiratory electron transport chain.[1]

In aerobic organisms undergoing respiration, electrons are shuttled to an electron transport chain, and the final electron acceptor is oxygen. Molecular oxygen is a high-energy [2] oxidizing agent and, therefore, is an excellent electron acceptor. Anaerobes instead use lower-energy, less-oxidizing substances such as nitrate (NO−
3), fumarate (C
4H
2O2−
4), sulfate (SO2−
4), or elemental sulfur (S). These terminal electron acceptors have smaller reduction potentials than O2 and release less energy per oxidized molecule. Therefore, anaerobic respiration is less efficient than aerobic.

As compared with fermentation

Anaerobic cellular respiration and fermentation generate ATP in very different ways, and the terms should not be treated as synonyms. Cellular respiration (both aerobic and anaerobic) uses highly reduced chemical compounds such as NADH and FADH2 (for example produced during glycolysis and the citric acid cycle) to establish an electrochemical gradient (often a proton gradient) across a membrane. This results in an electrical potential or ion concentration difference across the membrane. The reduced chemical compounds are oxidized by a series of respiratory integral membrane proteins with sequentially increasing reduction potentials, with the final electron acceptor being oxygen (in aerobic respiration) or another chemical substance (in anaerobic respiration). A proton motive force drives protons down the gradient (across the membrane) through the proton channel of ATP synthase. The resulting current drives ATP synthesis from ADP and inorganic phosphate.

Fermentation, in contrast, does not use an electrochemical gradient but instead uses only substrate-level phosphorylation to produce ATP. The electron acceptor NAD+ is regenerated from NADH formed in oxidative steps of the fermentation pathway by the reduction of oxidized compounds. These oxidized compounds are often formed during the fermentation pathway itself, but may also be external. For example, in homofermentative lactic acid bacteria, NADH formed during the oxidation of glyceraldehyde-3-phosphate is oxidized back to NAD+ by the reduction of pyruvate to lactic acid at a later stage in the pathway. In yeast, acetaldehyde is reduced to ethanol to regenerate NAD+.

There are two important anaerobic microbial methane formation pathways, through carbon dioxide / bicarbonate (HCO−
3) reduction (respiration) or acetate fermentation.[3]

Ecological importance

Anaerobic respiration is a critical component of the global nitrogen, iron, sulfur, and carbon cycles through the reduction of the oxyanions of nitrogen, sulfur, and carbon to more-reduced compounds. The biogeochemical cycling of these compounds, which depends upon anaerobic respiration, significantly impacts the carbon cycle and global warming. Anaerobic respiration occurs in many environments, including freshwater and marine sediments, soil, subsurface aquifers, deep subsurface environments, and biofilms. Even environments, such as soil, that contain oxygen also have micro-environments that lack oxygen due to the slow diffusion characteristics of oxygen gas.

An example of the ecological importance of anaerobic respiration is the use of nitrate as a terminal electron acceptor, or dissimilatory denitrification, which is the main route by which fixed nitrogen is returned to the atmosphere as molecular nitrogen gas.[4] The denitrification process is also very important in host-microbe interactions. Similar to mitochondria in oxygen-respiring microorganisms, some single-cellular anaerobic ciliates use denitrifying endosymbionts to gain energy.[5] Another example is methanogenesis, a form of carbon-dioxide respiration, that is used to produce methane gas by anaerobic digestion. Biogenic methane is used as a sustainable alternative to fossil fuels. On the negative side, uncontrolled methanogenesis in landfill sites releases large volumes of methane into the atmosphere, where it acts as a powerful greenhouse gas.[6] Sulfate respiration produces hydrogen sulfide, which is responsible for the characteristic 'rotten egg' smell of coastal wetlands and has the capacity to precipitate heavy metal ions from solution, leading to the deposition of sulfidic metal ores.[7]

Economic relevance

Dissimilatory denitrification is widely used in the removal of nitrate and nitrite from municipal wastewater. An excess of nitrate can lead to eutrophication of waterways into which treated water is released. Elevated nitrite levels in drinking water can lead to problems due to its toxicity. Denitrification converts both compounds into harmless nitrogen gas.[8]

Anaerobic Denitrification (ETC System)

The model above shows the process of anaerobic respiration through denitrification, which uses nitrogen (in the form of nitrate, NO−
3) as the electron acceptor. NO−
3 goes through respiratory dehydrogenase and reduces through each step from the ubiquinose through the bc1 complex through the ATP synthase protein as well. Each reductase removes oxygen step by step so that the final product of anaerobic respiration is N2.

1. Cytoplasm
2. Periplasm Compare to the aerobic electron transport chain.

Specific types of anaerobic respiration are also critical in bioremediation, which uses microorganisms to convert toxic chemicals into less-harmful molecules to clean up contaminated beaches, aquifers, lakes, and oceans. For example, toxic arsenate or selenate can be reduced to less toxic compounds by various anaerobic bacteria via anaerobic respiration. The reduction of chlorinated chemical pollutants, such as vinyl chloride and carbon tetrachloride, also occurs through anaerobic respiration.

Anaerobic respiration is useful in generating electricity in microbial fuel cells, which employ bacteria that respire solid electron acceptors (such as oxidized iron) to transfer electrons from reduced compounds to an electrode and release the energy of oxygen at the other electrode. This process can simultaneously degrade organic carbon waste and generate electricity.[9]

Examples of electron acceptors in respiration

Type Lifestyle Electron acceptor Products Eo′ (V) Example organisms
Aerobic respiration Obligate aerobes and facultative anaerobes O2 H2O, CO2 +0.82 Aerobic prokaryotes
Perchlorate respiration Facultative anaerobes ClO−
4, ClO−
3		 H2O, O2, Cl− +0.797 Azospira suillum, Sedimenticola selenatireducens, Sedimenticola thiotaurini, and other gram negative prokaryotes[10]
Iodate respiration Facultative anaerobes IO−
3		 H2O, H2O2, I− +0.72 Denitromonas,[11] Azoarcus, Pseudomonas, and other prokaryotes[12]
Iron reduction Facultative anaerobes and obligate anaerobes Fe(III) Fe(II) +0.75 Organisms within the order Desulfuromonadales (such as Geobacter, Geothermobacter, Geopsychrobacter, Pelobacter) and Shewanella species [13]
Manganese Facultative anaerobes and obligate anaerobes Mn(IV) Mn(II) Desulfuromonadales and Shewanella species [13]
Cobalt reduction Facultative anaerobes and obligate anaerobes Co(III) Co(II) Geobacter sulfurreducens
Uranium reduction Facultative anaerobes and obligate anaerobes U(VI) U(IV) Geobacter metallireducens, Shewanella oneidensis[14]
Nitrate reduction (denitrification) Facultative anaerobes Nitrate NO−
3		 (Ultimately) N2 +0.40 Paracoccus denitrificans, Escherichia coli
Fumarate respiration Facultative anaerobes Fumarate Succinate +0.03 Escherichia coli
Sulfate respiration Obligate anaerobes Sulfate SO2−
4		 Sulfide HS− −0.22 Many Deltaproteobacteria species in the orders Desulfobacterales, Desulfovibrionales, and Syntrophobacterales
Methanogenesis (carbon dioxide reduction) Methanogens Carbon dioxide CO2 Methane CH4 −0.25 Methanosarcina barkeri
Sulfur respiration (sulfur reduction) Facultative anaerobes and obligate anaerobes Sulfur S0 Sulfide HS− −0.27 Desulfuromonadales
Acetogenesis (carbon dioxide reduction) Obligate anaerobes Carbon dioxide CO2 Acetate −0.30 Acetobacterium woodii
Dehalorespiration Facultative anaerobes and obligate anaerobes Halogenated organic compounds R–X Halide ions and dehalogenated compound X− + R–H +0.25 – +0.60[15] Dehalococcoides and Dehalobacter species

See also

  • Hydrogenosomes and mitosomes
  • Anaerobic digestion[16]
  • Microbial fuel cell
  • Standard electrode potential (data page)
  • Table of standard reduction potentials for half-reactions important in biochemistry
  • Lithotrophs

Further reading

  • Gregory, Kelvin B.; Bond, Daniel R.; Lovley, Derek R. (June 2004). "Graphite electrodes as electron donors for anaerobic respiration". Environmental Microbiology. 6 (6): 596–604. doi:10.1111/j.1462-2920.2004.00593.x. ISSN 1462-2912. PMID 15142248.

References

  1. ↑ Slonczewski, Joan L.; Foster, John W. (2011). Microbiology: An Evolving Science (2nd ed.). New York: W.W. Norton. p. 166. ISBN 9780393934472.
  2. ↑ Schmidt-Rohr, K. (2020). "Oxygen Is the High-Energy Molecule Powering Complex Multicellular Life: Fundamental Corrections to Traditional Bioenergetics". ACS Omega. 5 (5): 2221–2233. doi:10.1021/acsomega.9b03352. PMC 7016920. PMID 32064383.
  3. ↑ Sapart; et al. (2017). "The origin of methane in the East Siberian Arctic Shelf unraveled with triple isotope analysis". Biogeosciences. 14 (9): 2283–2292. Bibcode:2017BGeo...14.2283S. doi:10.5194/bg-14-2283-2017.
  4. ↑ Simon, Jörg; Klotz, Martin G. (2013-02-01). "Diversity and evolution of bioenergetic systems involved in microbial nitrogen compound transformations". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1827 (2): 114–135. doi:10.1016/j.bbabio.2012.07.005. PMID 22842521.
  5. ↑ Graf, Jon S.; Schorn, Sina; Kitzinger, Katharina; Ahmerkamp, Soeren; Woehle, Christian; Huettel, Bruno; Schubert, Carsten J.; Kuypers, Marcel M. M.; Milucka, Jana (3 March 2021). "Anaerobic endosymbiont generates energy for ciliate host by denitrification". Nature. 591 (7850): 445–450. Bibcode:2021Natur.591..445G. doi:10.1038/s41586-021-03297-6. PMC 7969357. PMID 33658719.
  6. ↑ Bogner, Jean; Pipatti, Riitta; Hashimoto, Seiji; Diaz, Cristobal; Mareckova, Katarina; Diaz, Luis; Kjeldsen, Peter; Monni, Suvi; Faaij, Andre (2008-02-01). "Mitigation of global greenhouse gas emissions from waste: conclusions and strategies from the Intergovernmental Panel on Climate Change (IPCC) Fourth Assessment Report. Working Group III (Mitigation)". Waste Management & Research. 26 (1): 11–32. doi:10.1177/0734242x07088433. ISSN 0734-242X. PMID 18338699. S2CID 29740189.
  7. ↑ Pester, Michael; Knorr, Klaus-Holger; Friedrich, Michael W.; Wagner, Michael; Loy, Alexander (2012-01-01). "Sulfate-reducing microorganisms in wetlands – fameless actors in carbon cycling and climate change". Frontiers in Microbiology. 3: 72. doi:10.3389/fmicb.2012.00072. ISSN 1664-302X. PMC 3289269. PMID 22403575.
  8. ↑ Nancharaiah, Y. V.; Venkata Mohan, S.; Lens, P. N. L. (2016-09-01). "Recent advances in nutrient removal and recovery in biological and bioelectrochemical systems". Bioresource Technology. 215: 173–185. doi:10.1016/j.biortech.2016.03.129. ISSN 1873-2976. PMID 27053446.
  9. ↑ Xu, Bojun; Ge, Zheng; He, Zhen (2015-05-15). "Sediment microbial fuel cells for wastewater treatment: challenges and opportunities". Environmental Science: Water Research & Technology. 1 (3): 279–284. doi:10.1039/c5ew00020c. ISSN 2053-1419.
  10. ↑ Melnyk, Ryan A.; Engelbrektson, Anna; Clark, Iain C.; Carlson, Hans K.; Byrne-Bailey, Kathy; Coates, John D. (2011). "Identification of a Perchlorate Reduction Genomic Island with Novel Regulatory and Metabolic Genes". Applied and Environmental Microbiology. 77 (20): 7401–7404. Bibcode:2011ApEnM..77.7401M. doi:10.1128/AEM.05758-11. PMC 3194888. PMID 21856823.
  11. ↑ Reyes-Umana, Victor; Henning, Zachary; Lee, Kristina; Barnum, Tyler P.; Coates, John D. (2021-07-02). "Genetic and phylogenetic analysis of dissimilatory iodate-reducing bacteria identifies potential niches across the world's oceans". The ISME Journal. 16: 38–49. doi:10.1038/s41396-021-01034-5. ISSN 1751-7370. PMID 34215855. S2CID 235722250.
  12. ↑ Reyes-Umana, Victor; Henning, Zachary; Lee, Kristina; Barnum, Tyler; Coates, John (2020). "Genetic and phylogenetic analysis of dissimilatory iodate-reducing bacteria identifies potential niches across the world's oceans". bioRxiv 10.1101/2020.12.28.424624.
  13. 1 2 Richter, Katrin; Schicklberger, Marcus; Gescher, Johannes (2012-02-01). "Dissimilatory reduction of extracellular electron acceptors in anaerobic respiration". Applied and Environmental Microbiology. 78 (4): 913–921. Bibcode:2012ApEnM..78..913R. doi:10.1128/AEM.06803-11. ISSN 1098-5336. PMC 3273014. PMID 22179232.
  14. ↑ Wall, Judy D.; Krumholz, Lee R. (13 October 2006). "Uranium Reduction". Annual Review of Microbiology. 60: 149–166. doi:10.1146/annurev.micro.59.030804.121357. PMID 16704344.
  15. ↑ Holliger, C.; Wohlfarth, G.; Diekert, G. (1998). "Reductive dechlorination in the energy metabolism of anaerobic bacteria" (PDF). FEMS Microbiology Reviews. 22 (5): 383. doi:10.1111/j.1574-6976.1998.tb00377.x.
  16. ↑ Lovley, Derek R.; Fraga, Jocelyn L.; Coates, John D.; Blunt‐Harris, Elizabeth L. (1999). "Humics as an electron donor for anaerobic respiration". Environmental Microbiology. 1 (1): 89–98. doi:10.1046/j.1462-2920.1999.00009.x. PMID 11207721.
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